The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia
Purpose: Acute myeloid leukemia (AML) is known to be an invasive and highly lethal hematological malignancy in adults and children. Resistance to the present treatments, including radiotherapy and chemotherapy with their side effects and telomere length shortening are the main cause of the mortality...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2023-01-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-13-170.pdf |
| _version_ | 1797673476542169088 |
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| author | Khadijeh Dizaji Asl Ali Rafat Ali Akbar Movassaghpour Hojjatollah Nozad Charoudeh Hamid Tayefi Nasrabadi |
| author_facet | Khadijeh Dizaji Asl Ali Rafat Ali Akbar Movassaghpour Hojjatollah Nozad Charoudeh Hamid Tayefi Nasrabadi |
| author_sort | Khadijeh Dizaji Asl |
| collection | DOAJ |
| description | Purpose: Acute myeloid leukemia (AML) is known to be an invasive and highly lethal hematological malignancy in adults and children. Resistance to the present treatments, including radiotherapy and chemotherapy with their side effects and telomere length shortening are the main cause of the mortality in AML patients. Telomeres sequence which are located at the end of eukaryotic chromosome play pivotal role in genomic stability. Recent studies have shown that apoptosis process is blocked in AML patient by the excessive telomerase activity in cancerous blasts. Therefore, the find of effective ways to prevent disease progression has been considered by the researchers. Natural killer (NK) cells as granular effector cells play a critical role in elimination of abnormal and tumor cells. Given that the cytotoxic function of NK cells is disrupted in the AML patients, we investigated the effect of telomerase inhibitors on NK cell differentiation. Methods: To evaluate telomerase inhibition on NK cell differentiation, the expression of CD105, CD56, CD57, and KIRs was evaluated in CD34+derived NK cells after incubation of them with BIBR1532. Results: The results showed that the expression of CD105, CD56, CD57, and KIRs receptors reduces after telomerase inhibition. According to these findings, BIBR1532 affected the final differentiation of NK cells. Conclusion: The results revealed that telomerase inhibitor drugs suppress cancer cell progression in a NK cells-independent process. |
| first_indexed | 2024-03-11T21:45:01Z |
| format | Article |
| id | doaj.art-d8dff0a3344846d081c885d878f82c2a |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-03-11T21:45:01Z |
| publishDate | 2023-01-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-d8dff0a3344846d081c885d878f82c2a2023-09-26T10:13:34ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082023-01-0113117017510.34172/apb.2023.018apb-32834The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid LeukemiaKhadijeh Dizaji Asl0Ali Rafat1Ali Akbar Movassaghpour2Hojjatollah Nozad Charoudeh3Hamid Tayefi Nasrabadi4Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Purpose: Acute myeloid leukemia (AML) is known to be an invasive and highly lethal hematological malignancy in adults and children. Resistance to the present treatments, including radiotherapy and chemotherapy with their side effects and telomere length shortening are the main cause of the mortality in AML patients. Telomeres sequence which are located at the end of eukaryotic chromosome play pivotal role in genomic stability. Recent studies have shown that apoptosis process is blocked in AML patient by the excessive telomerase activity in cancerous blasts. Therefore, the find of effective ways to prevent disease progression has been considered by the researchers. Natural killer (NK) cells as granular effector cells play a critical role in elimination of abnormal and tumor cells. Given that the cytotoxic function of NK cells is disrupted in the AML patients, we investigated the effect of telomerase inhibitors on NK cell differentiation. Methods: To evaluate telomerase inhibition on NK cell differentiation, the expression of CD105, CD56, CD57, and KIRs was evaluated in CD34+derived NK cells after incubation of them with BIBR1532. Results: The results showed that the expression of CD105, CD56, CD57, and KIRs receptors reduces after telomerase inhibition. According to these findings, BIBR1532 affected the final differentiation of NK cells. Conclusion: The results revealed that telomerase inhibitor drugs suppress cancer cell progression in a NK cells-independent process.https://apb.tbzmed.ac.ir/PDF/apb-13-170.pdfnatural killer cellstelomerasetelomerase inhibitoracute myeloid leukemia |
| spellingShingle | Khadijeh Dizaji Asl Ali Rafat Ali Akbar Movassaghpour Hojjatollah Nozad Charoudeh Hamid Tayefi Nasrabadi The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia Advanced Pharmaceutical Bulletin natural killer cells telomerase telomerase inhibitor acute myeloid leukemia |
| title | The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia |
| title_full | The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia |
| title_fullStr | The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia |
| title_full_unstemmed | The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia |
| title_short | The Effect of Telomerase Inhibition on NK Cell Activity in Acute Myeloid Leukemia |
| title_sort | effect of telomerase inhibition on nk cell activity in acute myeloid leukemia |
| topic | natural killer cells telomerase telomerase inhibitor acute myeloid leukemia |
| url | https://apb.tbzmed.ac.ir/PDF/apb-13-170.pdf |
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