Chitinases from pitcher fluid of <i>Nepenthes distillatoria</i>
Chitinases belong to a diverse family of enzymes among many other hydrolytic enzymes of the pitcher fluid of <em>Nepenthes</em> sp. There are no existing studies on identification of chitinases in <em>Nepenthes distillatoria</em> the only representative of the genus <em>...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Science, University of Peradeniya, Sri Lanka
2019-09-01
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| Series: | Ceylon Journal of Science |
| Subjects: | |
| Online Access: | https://cjs.sljol.info/articles/7648 |
| Summary: | Chitinases belong to a diverse family of enzymes among many other hydrolytic enzymes of the pitcher fluid of <em>Nepenthes</em> sp. There are no existing studies on identification of chitinases in <em>Nepenthes distillatoria</em> the only representative of the genus <em>Nepenthes</em> in Sri Lanka. In this study an assay procedure was developed and optimized to detect the chitinolytic activity in the pitcher fluid of <em>N. distillatoria</em> using colloidal Chitin Azure as the substrate. Crude <em>Nepenthes</em> pitcher fluid was subjected to DEAE Cellulose chromatography in an attempt to partially purify and separate chitinases. The optimum pH, temperature and incubation time for the chitinolytic activity were 5.0, 27 °C and 30 min, respectively. DEAE Cellulose Chromatography revealed the presence of more than one type of chitinases within the pitcher juice. Further studies are required to characterize and confirm the origin/s of these chitinases. |
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| ISSN: | 2513-2814 2513-230X |