Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities
Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, we...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/4/456 |
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| author | Ding-Tao Wu Wen Liu Mei-Lin Xian Gang Du Xin Liu Jing-Jing He Ping Wang Wen Qin Li Zhao |
| author_facet | Ding-Tao Wu Wen Liu Mei-Lin Xian Gang Du Xin Liu Jing-Jing He Ping Wang Wen Qin Li Zhao |
| author_sort | Ding-Tao Wu |
| collection | DOAJ |
| description | Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from <i>Hovenia dulcis</i>. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry. |
| first_indexed | 2024-03-10T20:37:13Z |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T20:37:13Z |
| publishDate | 2020-04-01 |
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| series | Foods |
| spelling | doaj.art-d8e28b48ac9a46b38a0a82d054421c242023-11-19T20:59:23ZengMDPI AGFoods2304-81582020-04-019445610.3390/foods9040456Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro BioactivitiesDing-Tao Wu0Wen Liu1Mei-Lin Xian2Gang Du3Xin Liu4Jing-Jing He5Ping Wang6Wen Qin7Li Zhao8College of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaSichuan Provincial Institute for Food and Drug Control, Chengdu 611731, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaCollege of Food Science, Sichuan Agricultural University, Ya’an 625014, Sichuan, ChinaSeven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from <i>Hovenia dulcis</i>. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.https://www.mdpi.com/2304-8158/9/4/456polyphenolic-protein-polysaccharide<i>Hovenia dulcis</i>extraction methodphysicochemical propertiesin vitro bioactivity |
| spellingShingle | Ding-Tao Wu Wen Liu Mei-Lin Xian Gang Du Xin Liu Jing-Jing He Ping Wang Wen Qin Li Zhao Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities Foods polyphenolic-protein-polysaccharide <i>Hovenia dulcis</i> extraction method physicochemical properties in vitro bioactivity |
| title | Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities |
| title_full | Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities |
| title_fullStr | Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities |
| title_full_unstemmed | Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities |
| title_short | Polyphenolic-Protein-Polysaccharide Complexes from <i>Hovenia dulcis</i>: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities |
| title_sort | polyphenolic protein polysaccharide complexes from i hovenia dulcis i insights into extraction methods on their physicochemical properties and in vitro bioactivities |
| topic | polyphenolic-protein-polysaccharide <i>Hovenia dulcis</i> extraction method physicochemical properties in vitro bioactivity |
| url | https://www.mdpi.com/2304-8158/9/4/456 |
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