Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters
In this work, a label-free fluorescent detection method for glyphosate, based on DNA-templated silver nanoclusters (DNA-Ag NCs) and a Cu<sup>2+</sup>-ion-modulated strategy, was developed. In the presence of Cu<sup>2+</sup>, the fluorescence of the DNA-Ag NCs was quenched. Gl...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/832 |
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| author | Yuliang Cheng Guowen Li Xiufang Huang Zhijuan Qian Chifang Peng |
| author_facet | Yuliang Cheng Guowen Li Xiufang Huang Zhijuan Qian Chifang Peng |
| author_sort | Yuliang Cheng |
| collection | DOAJ |
| description | In this work, a label-free fluorescent detection method for glyphosate, based on DNA-templated silver nanoclusters (DNA-Ag NCs) and a Cu<sup>2+</sup>-ion-modulated strategy, was developed. In the presence of Cu<sup>2+</sup>, the fluorescence of the DNA-Ag NCs was quenched. Glyphosate can restore the fluorescence of DNA-Ag NCs. By analyzing the storage stability of the obtained DNA-Ag NCs using different DNA templates, specific DNA-Ag NCs were selected for the construction of the glyphosate sensor. The ultrasensitive detection of glyphosate was achieved by optimizing the buffer pH and Cu<sup>2+</sup> concentration. The sensing of glyphosate demonstrated a linear response in the range of 1.0–50 ng/mL. The limit of detection (LOD) was 0.2 ng/mL. The proposed method was successfully applied in the detection of glyphosate in a real sample, indicating its high application potential for glyphosate detection. |
| first_indexed | 2024-03-09T20:36:23Z |
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| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:36:23Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
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| series | Biosensors |
| spelling | doaj.art-d8e6cbb36a6b4354b3bb318250c01a902023-11-23T23:11:23ZengMDPI AGBiosensors2079-63742022-10-01121083210.3390/bios12100832Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver NanoclustersYuliang Cheng0Guowen Li1Xiufang Huang2Zhijuan Qian3Chifang Peng4State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, ChinaNanjing Customs District Light Industry 375 Productsand Children’s Products Inspection Center, Yangzhou 225009, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, ChinaIn this work, a label-free fluorescent detection method for glyphosate, based on DNA-templated silver nanoclusters (DNA-Ag NCs) and a Cu<sup>2+</sup>-ion-modulated strategy, was developed. In the presence of Cu<sup>2+</sup>, the fluorescence of the DNA-Ag NCs was quenched. Glyphosate can restore the fluorescence of DNA-Ag NCs. By analyzing the storage stability of the obtained DNA-Ag NCs using different DNA templates, specific DNA-Ag NCs were selected for the construction of the glyphosate sensor. The ultrasensitive detection of glyphosate was achieved by optimizing the buffer pH and Cu<sup>2+</sup> concentration. The sensing of glyphosate demonstrated a linear response in the range of 1.0–50 ng/mL. The limit of detection (LOD) was 0.2 ng/mL. The proposed method was successfully applied in the detection of glyphosate in a real sample, indicating its high application potential for glyphosate detection.https://www.mdpi.com/2079-6374/12/10/832label freefluorescenceturn onDNACu<sup>2+</sup>silver nanoclusters |
| spellingShingle | Yuliang Cheng Guowen Li Xiufang Huang Zhijuan Qian Chifang Peng Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters Biosensors label free fluorescence turn on DNA Cu<sup>2+</sup> silver nanoclusters |
| title | Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters |
| title_full | Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters |
| title_fullStr | Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters |
| title_full_unstemmed | Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters |
| title_short | Label-Free Fluorescent Turn-On Glyphosate Sensing Based on DNA-Templated Silver Nanoclusters |
| title_sort | label free fluorescent turn on glyphosate sensing based on dna templated silver nanoclusters |
| topic | label free fluorescence turn on DNA Cu<sup>2+</sup> silver nanoclusters |
| url | https://www.mdpi.com/2079-6374/12/10/832 |
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