Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro Study
Lactic acid bacteria (LAB) are widely used probiotics and offer promising prospects for increasing the viability of honeybees. Thus, the probiotic potential of 10 LAB strains was determined, which in our previous studies showed the most potent protective abilities. In the current study, we investiga...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/6/1059 |
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| author | Aleksandra Leska Adriana Nowak Justyna Rosicka-Kaczmarek Małgorzata Ryngajłło Karolina Henryka Czarnecka-Chrebelska |
| author_facet | Aleksandra Leska Adriana Nowak Justyna Rosicka-Kaczmarek Małgorzata Ryngajłło Karolina Henryka Czarnecka-Chrebelska |
| author_sort | Aleksandra Leska |
| collection | DOAJ |
| description | Lactic acid bacteria (LAB) are widely used probiotics and offer promising prospects for increasing the viability of honeybees. Thus, the probiotic potential of 10 LAB strains was determined, which in our previous studies showed the most potent protective abilities. In the current study, we investigated various properties of probiotic candidates. The tested LAB strains varied in susceptibility to tested antibiotics. Isolates showed high viability in sugar syrups and gastrointestinal conditions. None of the LAB strains exhibited β-hemolytic activity, mutual antagonism, mucin degradation, hydrogen peroxide production capacity, or bile salt hydrolase (BSH) activity. Additionally, the cytotoxicity of LAB cell-free supernatants (CFS) was assessed, as well as the effect of CFS from <i>P. pentosaceus</i> 14/1 on the cytotoxicity of coumaphos and chlorpyrifos in the Caco-2 cell line. The viability of Caco-2 cells reached up to 89.81% in the presence of the highest concentration of CFS. Furthermore, LAB metabolites decreased the cytotoxicity of insecticides (up to 19.32%) thus demonstrating cytoprotective activity. All tested LAB strains produced lactic, acetic, and malonic acids. This research allowed the selection of the most effective LAB strains, in terms of probiosis, for future in vivo studies aimed at developing an ecologically protective biopreparation for honeybees. |
| first_indexed | 2024-03-11T07:01:59Z |
| format | Article |
| id | doaj.art-d8e978b385c94818ba19ae1df57859bc |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:01:59Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-d8e978b385c94818ba19ae1df57859bc2023-11-17T09:11:47ZengMDPI AGAnimals2076-26152023-03-01136105910.3390/ani13061059Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro StudyAleksandra Leska0Adriana Nowak1Justyna Rosicka-Kaczmarek2Małgorzata Ryngajłło3Karolina Henryka Czarnecka-Chrebelska4Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, PolandDepartment of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, PolandInstitute of Food Technology and Analysis, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-537 Lodz, PolandInstitute of Molecular and Industrial Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 2/22, 90-573 Lodz, PolandDepartment of Biomedicine and Genetics, Chair of Biology and Medical Microbiology, Medical University of Lodz, 5 Mazowiecka Str. (A-6 Building), 92-215 Lodz, PolandLactic acid bacteria (LAB) are widely used probiotics and offer promising prospects for increasing the viability of honeybees. Thus, the probiotic potential of 10 LAB strains was determined, which in our previous studies showed the most potent protective abilities. In the current study, we investigated various properties of probiotic candidates. The tested LAB strains varied in susceptibility to tested antibiotics. Isolates showed high viability in sugar syrups and gastrointestinal conditions. None of the LAB strains exhibited β-hemolytic activity, mutual antagonism, mucin degradation, hydrogen peroxide production capacity, or bile salt hydrolase (BSH) activity. Additionally, the cytotoxicity of LAB cell-free supernatants (CFS) was assessed, as well as the effect of CFS from <i>P. pentosaceus</i> 14/1 on the cytotoxicity of coumaphos and chlorpyrifos in the Caco-2 cell line. The viability of Caco-2 cells reached up to 89.81% in the presence of the highest concentration of CFS. Furthermore, LAB metabolites decreased the cytotoxicity of insecticides (up to 19.32%) thus demonstrating cytoprotective activity. All tested LAB strains produced lactic, acetic, and malonic acids. This research allowed the selection of the most effective LAB strains, in terms of probiosis, for future in vivo studies aimed at developing an ecologically protective biopreparation for honeybees.https://www.mdpi.com/2076-2615/13/6/1059probiotics<i>Apis mellifera</i> L.honeybeeSCFAsugar syrupantibiotics |
| spellingShingle | Aleksandra Leska Adriana Nowak Justyna Rosicka-Kaczmarek Małgorzata Ryngajłło Karolina Henryka Czarnecka-Chrebelska Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro Study Animals probiotics <i>Apis mellifera</i> L. honeybee SCFA sugar syrup antibiotics |
| title | Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro Study |
| title_full | Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro Study |
| title_fullStr | Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro Study |
| title_full_unstemmed | Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro Study |
| title_short | Characterization and Protective Properties of Lactic Acid Bacteria Intended to Be Used in Probiotic Preparation for Honeybees (<i>Apis mellifera</i> L.)—An In Vitro Study |
| title_sort | characterization and protective properties of lactic acid bacteria intended to be used in probiotic preparation for honeybees i apis mellifera i l an in vitro study |
| topic | probiotics <i>Apis mellifera</i> L. honeybee SCFA sugar syrup antibiotics |
| url | https://www.mdpi.com/2076-2615/13/6/1059 |
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