Serum from morbidly obese patients affects melanoma cell behavior in vitro
Abstract Here we examined whether serum from obese patients could create a growth-enhancing microenvironment that alters gene expression in BRAF- and NRAS-mutated melanoma cell lines. SK-Mel-28 (BRAF-mutated) and Sk-Mel-147 (NRAS-mutated) cells were treated with pooled serum from 10 severely obese p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-06-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100583&lng=en&tlng=en |
| _version_ | 1811243984065921024 |
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| author | Débora Mocellin Letícia de Oliveira Souza Bratti Adny Henrique Silva Laura Sartori Assunção Iara Fabricia Kretzer Fabíola Branco Filippin-Monteiro |
| author_facet | Débora Mocellin Letícia de Oliveira Souza Bratti Adny Henrique Silva Laura Sartori Assunção Iara Fabricia Kretzer Fabíola Branco Filippin-Monteiro |
| author_sort | Débora Mocellin |
| collection | DOAJ |
| description | Abstract Here we examined whether serum from obese patients could create a growth-enhancing microenvironment that alters gene expression in BRAF- and NRAS-mutated melanoma cell lines. SK-Mel-28 (BRAF-mutated) and Sk-Mel-147 (NRAS-mutated) cells were treated with pooled serum from 10 severely obese patients (BMI > 40 kg/m2), pooled serum from 6 healthy lean individuals (BMI = 18.5-24.9 kg/m2), or recombinant TNF-α. We found that obese patient serum enhanced migration capacity and increased NRAS expression levels in both BRAF- and NRAS-mutated melanoma cells. Although TNF-α is the major obesity-related cytokine and TNF-α levels were found to be increased in the serum of obese individuals, this cytokine made only a modest contribution to the migration capacity of melanoma cells. These results indicate that other components present in the serum of severely obese patients may be responsible for enhancing the migration capacity of melanoma cells. As TNF-α alone did not seem to significantly affect tumor cell behavior, anti-tumor strategies aimed at blocking TNF-α should be considered with caution in future studies, particularly when in vitro models are used as screening platforms for antitumor activity. |
| first_indexed | 2024-04-12T14:17:55Z |
| format | Article |
| id | doaj.art-d8ea397064d94b36b3651fc52f9090b8 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-12T14:17:55Z |
| publishDate | 2022-06-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-d8ea397064d94b36b3651fc52f9090b82022-12-22T03:29:40ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-06-015810.1590/s2175-979020201000x42e19375Serum from morbidly obese patients affects melanoma cell behavior in vitroDébora MocellinLetícia de Oliveira Souza BrattiAdny Henrique Silvahttps://orcid.org/0000-0002-8511-3973Laura Sartori AssunçãoIara Fabricia KretzerFabíola Branco Filippin-Monteirohttps://orcid.org/0000-0003-4313-1783Abstract Here we examined whether serum from obese patients could create a growth-enhancing microenvironment that alters gene expression in BRAF- and NRAS-mutated melanoma cell lines. SK-Mel-28 (BRAF-mutated) and Sk-Mel-147 (NRAS-mutated) cells were treated with pooled serum from 10 severely obese patients (BMI > 40 kg/m2), pooled serum from 6 healthy lean individuals (BMI = 18.5-24.9 kg/m2), or recombinant TNF-α. We found that obese patient serum enhanced migration capacity and increased NRAS expression levels in both BRAF- and NRAS-mutated melanoma cells. Although TNF-α is the major obesity-related cytokine and TNF-α levels were found to be increased in the serum of obese individuals, this cytokine made only a modest contribution to the migration capacity of melanoma cells. These results indicate that other components present in the serum of severely obese patients may be responsible for enhancing the migration capacity of melanoma cells. As TNF-α alone did not seem to significantly affect tumor cell behavior, anti-tumor strategies aimed at blocking TNF-α should be considered with caution in future studies, particularly when in vitro models are used as screening platforms for antitumor activity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100583&lng=en&tlng=enMelanomaTumor progressionInflammationMorbidly obesity |
| spellingShingle | Débora Mocellin Letícia de Oliveira Souza Bratti Adny Henrique Silva Laura Sartori Assunção Iara Fabricia Kretzer Fabíola Branco Filippin-Monteiro Serum from morbidly obese patients affects melanoma cell behavior in vitro Brazilian Journal of Pharmaceutical Sciences Melanoma Tumor progression Inflammation Morbidly obesity |
| title | Serum from morbidly obese patients affects melanoma cell behavior in vitro |
| title_full | Serum from morbidly obese patients affects melanoma cell behavior in vitro |
| title_fullStr | Serum from morbidly obese patients affects melanoma cell behavior in vitro |
| title_full_unstemmed | Serum from morbidly obese patients affects melanoma cell behavior in vitro |
| title_short | Serum from morbidly obese patients affects melanoma cell behavior in vitro |
| title_sort | serum from morbidly obese patients affects melanoma cell behavior in vitro |
| topic | Melanoma Tumor progression Inflammation Morbidly obesity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100583&lng=en&tlng=en |
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