Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation
Abstract To investigate the ovarian responses, ovarian and uterine hemodynamics, circulating ovarian hormones, and nitric oxide (NO) with their relations in superstimulated cows. Eight Holstein Friesian dry cows previously synchronized with CIDR underwent rectal Doppler ultrasound scanning and blood...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-023-03692-3 |
| _version_ | 1797452268285460480 |
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| author | Elshymaa A. Abdelnaby Abdulrhman K. Alhaider Amal M. Abo El-Maaty Refaat S. A. Ragab Adel A. Seida Diya A. El-Badry |
| author_facet | Elshymaa A. Abdelnaby Abdulrhman K. Alhaider Amal M. Abo El-Maaty Refaat S. A. Ragab Adel A. Seida Diya A. El-Badry |
| author_sort | Elshymaa A. Abdelnaby |
| collection | DOAJ |
| description | Abstract To investigate the ovarian responses, ovarian and uterine hemodynamics, circulating ovarian hormones, and nitric oxide (NO) with their relations in superstimulated cows. Eight Holstein Friesian dry cows previously synchronized with CIDR underwent rectal Doppler ultrasound scanning and blood sampling after administrating eCG (1500 I.U) on day 10 of the second ovulation (day -5). Cows were treated with 12.5 mg prostaglandin F2α (PGF2α) on days 10 and 17 after ovulation. Estradiol, progesterone, and NO were measured. Results showed that from ≥ 13 follicles, five follicles ovulated from both ovaries. The ovulated follicles increased antrum colored area and colored area % till day -1. The developed corpora lutea (CLs) attained similar diameter, area, colored area, and colored area % from day 2 till day 15. The peak point of velocity (PSV) of uterine arteries decreased while that of ovarian arteries increased from day -4 to day 0. Both ovarian arteries diameter, resistance index (RI), PSV, end velocity (EDV) and systolic/diastolic ratio (S/D) positively correlated (P < 0.0001), but their pulsatility index (PI) negatively correlated (P < 0.0001). The uterine arteries PI, RI, PSV, EDV, time average velocity (TAMV) and S/D negatively correlated (P < 0.0001) but their diameters positively correlated. Estradiol increased but progesterone decreased from day -5 till day 0. After ovulation, P4 reached maximum values on day 9 and started to decrease till day 19.NO showed one peak on day -3 and another one from day 3 to day 9. Conclusions: Blood flow of ovarian arteries is different from uterine arteries and depended on pre- or post-ovulation. |
| first_indexed | 2024-03-09T15:06:21Z |
| format | Article |
| id | doaj.art-d8eb8f419412425599ed4fb07f94d4da |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-03-09T15:06:21Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-d8eb8f419412425599ed4fb07f94d4da2023-11-26T13:38:33ZengBMCBMC Veterinary Research1746-61482023-10-0119111210.1186/s12917-023-03692-3Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulationElshymaa A. Abdelnaby0Abdulrhman K. Alhaider1Amal M. Abo El-Maaty2Refaat S. A. Ragab3Adel A. Seida4Diya A. El-Badry5Department of Clinical Sciences, College of Veterinary Medicine, King Faisal UniversityTheriogenology Department, Faculty of Veterinary Medicine, Cairo UniversityAnimal Reproduction and AI Department, Veterinary Division, National Research CentreDepartment of Clinical Sciences, College of Veterinary Medicine, King Faisal UniversityDepartment of Clinical Sciences, College of Veterinary Medicine, King Faisal UniversityArtificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute, Agriculture Research CenterAbstract To investigate the ovarian responses, ovarian and uterine hemodynamics, circulating ovarian hormones, and nitric oxide (NO) with their relations in superstimulated cows. Eight Holstein Friesian dry cows previously synchronized with CIDR underwent rectal Doppler ultrasound scanning and blood sampling after administrating eCG (1500 I.U) on day 10 of the second ovulation (day -5). Cows were treated with 12.5 mg prostaglandin F2α (PGF2α) on days 10 and 17 after ovulation. Estradiol, progesterone, and NO were measured. Results showed that from ≥ 13 follicles, five follicles ovulated from both ovaries. The ovulated follicles increased antrum colored area and colored area % till day -1. The developed corpora lutea (CLs) attained similar diameter, area, colored area, and colored area % from day 2 till day 15. The peak point of velocity (PSV) of uterine arteries decreased while that of ovarian arteries increased from day -4 to day 0. Both ovarian arteries diameter, resistance index (RI), PSV, end velocity (EDV) and systolic/diastolic ratio (S/D) positively correlated (P < 0.0001), but their pulsatility index (PI) negatively correlated (P < 0.0001). The uterine arteries PI, RI, PSV, EDV, time average velocity (TAMV) and S/D negatively correlated (P < 0.0001) but their diameters positively correlated. Estradiol increased but progesterone decreased from day -5 till day 0. After ovulation, P4 reached maximum values on day 9 and started to decrease till day 19.NO showed one peak on day -3 and another one from day 3 to day 9. Conclusions: Blood flow of ovarian arteries is different from uterine arteries and depended on pre- or post-ovulation.https://doi.org/10.1186/s12917-023-03692-3CowsDoppler indiceseCGOvarian arteryUterine artery |
| spellingShingle | Elshymaa A. Abdelnaby Abdulrhman K. Alhaider Amal M. Abo El-Maaty Refaat S. A. Ragab Adel A. Seida Diya A. El-Badry Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation BMC Veterinary Research Cows Doppler indices eCG Ovarian artery Uterine artery |
| title | Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation |
| title_full | Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation |
| title_fullStr | Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation |
| title_full_unstemmed | Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation |
| title_short | Ovarian and uterine arteries blood flow velocities waveform, hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation |
| title_sort | ovarian and uterine arteries blood flow velocities waveform hormones and nitric oxide in relation to ovulation in cows superstimulated with equine chorionic gonadotropin and luteolysis induction 10 and 17 days after ovulation |
| topic | Cows Doppler indices eCG Ovarian artery Uterine artery |
| url | https://doi.org/10.1186/s12917-023-03692-3 |
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