Anti-inflammatory effects of spermidine in lipopolysaccharide-stimulated BV2 microglial cells
<p>Abstract</p> <p>Background</p> <p>Spermidine, a naturally occurring polyamine, displays a wide variety of internal biological activities including cell growth and proliferation. However, the molecular mechanisms responsible for its anti-inflammatory activity have not...
| Autores principales: | , |
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| Formato: | Artículo |
| Lenguaje: | English |
| Publicado: |
BMC
2012-03-01
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| Colección: | Journal of Biomedical Science |
| Materias: | |
| Acceso en línea: | http://www.jbiomedsci.com/content/19/1/31 |
| Sumario: | <p>Abstract</p> <p>Background</p> <p>Spermidine, a naturally occurring polyamine, displays a wide variety of internal biological activities including cell growth and proliferation. However, the molecular mechanisms responsible for its anti-inflammatory activity have not yet been elucidated.</p> <p>Methods</p> <p>The anti-inflammatory properties of spermidine were studied using lipopolysaccharide (LPS)-stimulated murine BV2 microglia model. As inflammatory parameters, the production of nitric oxide (NO), prostaglandin E<sub>2 </sub>(PGE<sub>2</sub>), interleukin (IL)-6 and tumor necrosis factor (TNF)-α were evaluated. We also examined the spermidine's effect on the activity of nuclear factor-kappaB (NF-κB), and the phosphoinositide 3-kinase (PI3K)/Akt and mitogen-activated protein kinases (MAPKs) pathways.</p> <p>Results</p> <p>Pretreatment with spermidine prior to LPS treatment significantly inhibited excessive production of NO and PGE<sub>2 </sub>in a dose-dependent manner, and was associated with down-regulation of expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Spermidine treatment also attenuated the production of pro-inflammatory cytokines, including IL-6 and TNF-α, by suppressing their mRNA expressions. The mechanism underlying spermidine-mediated attenuation of inflammation in BV2 cells appeared to involve the suppression of translocation of NF-κB p65 subunit into the nucleus, and the phosphorylation of Akt and MAPKs.</p> <p>Conclusions</p> <p>The results indicate that spermidine appears to inhibit inflammation stimulated by LPS by blocking the NF-κB, PI3K/Akt and MAPKs signaling pathways in microglia.</p> |
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| ISSN: | 1021-7770 1423-0127 |