Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine
Simple, sensitive, accurate and inexpensive spectrophotometric methods have been developed for the determination of sulfamethoxazole (SMZ) in pure and dosage forms. This method is based on diazotization of primary amine group of sulfamethoxazole with sodium nitrite and hydrochloric acid followe...
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| Format: | Article |
| Language: | English |
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University of Baghdad
2017-04-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| Subjects: | |
| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/303 |
| _version_ | 1818269461135753216 |
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| author | Husam S. Khalaf Abdul Mohsin A. Al-Haidari Sarmad B. Dikran Alaa K. Mohammed |
| author_facet | Husam S. Khalaf Abdul Mohsin A. Al-Haidari Sarmad B. Dikran Alaa K. Mohammed |
| author_sort | Husam S. Khalaf |
| collection | DOAJ |
| description |
Simple, sensitive, accurate and inexpensive spectrophotometric methods have been developed for the determination of sulfamethoxazole (SMZ) in pure and dosage forms. This method is based on diazotization of primary amine group of sulfamethoxazole with sodium nitrite and hydrochloric acid followed by coupling with diphenylamine in acidic medium to obtain a stable blue colored dye and show a maximum absorption (max) at 530 nm. Different variables affecting the completion of reaction have been carefully optimized, following the classical univariate sequence and modified simplex method. Beer’s law is obeyed in the concentration range of (0.5-12.0 µg.mL-1) with molar absorptivity of 4.9617×104 L.mol-1.cm-1. The limit of detection was (0.108 µg.mL-1), and Sandell’s sensitivity value 5.1047 µg.cm-2. The proposed method was successfully applied to the determination of sulfamethoxazole in pharmaceutical preparation
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| first_indexed | 2024-12-12T20:54:45Z |
| format | Article |
| id | doaj.art-d8f03d26770f47c7b752483f2aab2e0b |
| institution | Directory Open Access Journal |
| issn | 1609-4042 2521-3407 |
| language | English |
| last_indexed | 2024-12-12T20:54:45Z |
| publishDate | 2017-04-01 |
| publisher | University of Baghdad |
| record_format | Article |
| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj.art-d8f03d26770f47c7b752483f2aab2e0b2022-12-22T00:12:19ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072017-04-01273Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with DiphenylamineHusam S. KhalafAbdul Mohsin A. Al-HaidariSarmad B. DikranAlaa K. Mohammed Simple, sensitive, accurate and inexpensive spectrophotometric methods have been developed for the determination of sulfamethoxazole (SMZ) in pure and dosage forms. This method is based on diazotization of primary amine group of sulfamethoxazole with sodium nitrite and hydrochloric acid followed by coupling with diphenylamine in acidic medium to obtain a stable blue colored dye and show a maximum absorption (max) at 530 nm. Different variables affecting the completion of reaction have been carefully optimized, following the classical univariate sequence and modified simplex method. Beer’s law is obeyed in the concentration range of (0.5-12.0 µg.mL-1) with molar absorptivity of 4.9617×104 L.mol-1.cm-1. The limit of detection was (0.108 µg.mL-1), and Sandell’s sensitivity value 5.1047 µg.cm-2. The proposed method was successfully applied to the determination of sulfamethoxazole in pharmaceutical preparation https://jih.uobaghdad.edu.iq/index.php/j/article/view/303Spectrophotometric, determination, Dosage forms, Sulfamethoxazole, Diazotization reaction, Coupling reaction, Diphenylamine. |
| spellingShingle | Husam S. Khalaf Abdul Mohsin A. Al-Haidari Sarmad B. Dikran Alaa K. Mohammed Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine Ibn Al-Haitham Journal for Pure and Applied Sciences Spectrophotometric, determination, Dosage forms, Sulfamethoxazole, Diazotization reaction, Coupling reaction, Diphenylamine. |
| title | Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine |
| title_full | Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine |
| title_fullStr | Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine |
| title_full_unstemmed | Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine |
| title_short | Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine |
| title_sort | spectrophotometric determination of sulfamethoxazole following simple diazotization and coupling with diphenylamine |
| topic | Spectrophotometric, determination, Dosage forms, Sulfamethoxazole, Diazotization reaction, Coupling reaction, Diphenylamine. |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/303 |
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