The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction
Burn-induced cardiac dysfunction is thought to involve mitochondrial dysfunction, although the mechanisms responsible are unclear. In this study, we used our established model of in vivo burn injury to understand the genetic evidence of burn-induced mitochondrial confusion dysfunction by describing...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/12/566 |
| _version_ | 1797545846245425152 |
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| author | Jake J. Wen Claire B. Cummins Taylor P. Williams Ravi S. Radhakrishnan |
| author_facet | Jake J. Wen Claire B. Cummins Taylor P. Williams Ravi S. Radhakrishnan |
| author_sort | Jake J. Wen |
| collection | DOAJ |
| description | Burn-induced cardiac dysfunction is thought to involve mitochondrial dysfunction, although the mechanisms responsible are unclear. In this study, we used our established model of in vivo burn injury to understand the genetic evidence of burn-induced mitochondrial confusion dysfunction by describing cardiac mitochondrial metabolism-related gene expression after burn. Cardiac tissue was collected at 24 hours after burn injury. An O2K respirometer system was utilized to measure the cardiac mitochondrial function. Oxidative phosphorylation complex activities were determined using enzyme activity assays. RT Profiler PCR array was used to identify the differential regulation of genes involved in mitochondrial biogenesis and metabolism. The quantitative qPCR and Western blotting were applied to validate the differentially expressed genes. Burn-induced cardiac mitochondrial dysfunction was supported by the finding of decreased state 3 respiration, decreased mitochondrial electron transport chain activity in complex I, III, IV, and V, and decreased mitochondrial DNA-encoded gene expression as well as decreased levels of the corresponding proteins after burn injury. Eighty-four mitochondrial metabolism-related gene profiles were measured. The mitochondrial gene profile showed that 29 genes related to mitochondrial energy and metabolism was differentially expressed. Of these 29 genes, 16 were more than 2-fold upregulated and 13 were more than 2-fold downregulated. All genes were validated using qPCR and partial genes were correlated with their protein levels. This study provides preliminary evidence that a large percentage of mitochondrial metabolism-related genes in cardiomyocytes were significantly affected by burn injury. |
| first_indexed | 2024-03-10T14:21:50Z |
| format | Article |
| id | doaj.art-d8f215dcf245417b8809235875b1ff10 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T14:21:50Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj.art-d8f215dcf245417b8809235875b1ff102023-11-20T23:22:06ZengMDPI AGBiomedicines2227-90592020-12-0181256610.3390/biomedicines8120566The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism DysfunctionJake J. Wen0Claire B. Cummins1Taylor P. Williams2Ravi S. Radhakrishnan3Department of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Surgery, University of Texas Medical Branch, Galveston, TX 77555, USABurn-induced cardiac dysfunction is thought to involve mitochondrial dysfunction, although the mechanisms responsible are unclear. In this study, we used our established model of in vivo burn injury to understand the genetic evidence of burn-induced mitochondrial confusion dysfunction by describing cardiac mitochondrial metabolism-related gene expression after burn. Cardiac tissue was collected at 24 hours after burn injury. An O2K respirometer system was utilized to measure the cardiac mitochondrial function. Oxidative phosphorylation complex activities were determined using enzyme activity assays. RT Profiler PCR array was used to identify the differential regulation of genes involved in mitochondrial biogenesis and metabolism. The quantitative qPCR and Western blotting were applied to validate the differentially expressed genes. Burn-induced cardiac mitochondrial dysfunction was supported by the finding of decreased state 3 respiration, decreased mitochondrial electron transport chain activity in complex I, III, IV, and V, and decreased mitochondrial DNA-encoded gene expression as well as decreased levels of the corresponding proteins after burn injury. Eighty-four mitochondrial metabolism-related gene profiles were measured. The mitochondrial gene profile showed that 29 genes related to mitochondrial energy and metabolism was differentially expressed. Of these 29 genes, 16 were more than 2-fold upregulated and 13 were more than 2-fold downregulated. All genes were validated using qPCR and partial genes were correlated with their protein levels. This study provides preliminary evidence that a large percentage of mitochondrial metabolism-related genes in cardiomyocytes were significantly affected by burn injury.https://www.mdpi.com/2227-9059/8/12/566burn injurycardiac dysfunctiongene profilingmitochondrial metabolismoxygen consumption |
| spellingShingle | Jake J. Wen Claire B. Cummins Taylor P. Williams Ravi S. Radhakrishnan The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction Biomedicines burn injury cardiac dysfunction gene profiling mitochondrial metabolism oxygen consumption |
| title | The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction |
| title_full | The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction |
| title_fullStr | The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction |
| title_full_unstemmed | The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction |
| title_short | The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction |
| title_sort | genetic evidence of burn induced cardiac mitochondrial metabolism dysfunction |
| topic | burn injury cardiac dysfunction gene profiling mitochondrial metabolism oxygen consumption |
| url | https://www.mdpi.com/2227-9059/8/12/566 |
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