Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean
Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M2 population....
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| Format: | Article |
| Language: | English |
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Elsevier
2016-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311915613120 |
| _version_ | 1818893207349493760 |
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| author | Wen CHENG Jin-shan GAO Xing-xing FENG Qun SHAO Su-xin YANG Xian-zhong FENG |
| author_facet | Wen CHENG Jin-shan GAO Xing-xing FENG Qun SHAO Su-xin YANG Xian-zhong FENG |
| author_sort | Wen CHENG |
| collection | DOAJ |
| description | Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M2 population. Two of these mutants, Gmdwf1 (Glycine max dwarf 1) and Gmdwf2 (Glycine max dwarf 2), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1. |
| first_indexed | 2024-12-19T18:08:56Z |
| format | Article |
| id | doaj.art-d8f32aa963f14d848c33c99f21cfad24 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T18:08:56Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-d8f32aa963f14d848c33c99f21cfad242022-12-21T20:11:23ZengElsevierJournal of Integrative Agriculture2095-31192016-10-01151022282236Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybeanWen CHENG0Jin-shan GAO1Xing-xing FENG2Qun SHAO3Su-xin YANG4Xian-zhong FENG5Key Laboratory of Systems Biology in Universities of Shandong/College of Life Science, Shandong Normal University, Jinan 250014, P.R. ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R. ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R. ChinaKey Laboratory of Systems Biology in Universities of Shandong/College of Life Science, Shandong Normal University, Jinan 250014, P.R. ChinaKey Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R. ChinaKey Laboratory of Systems Biology in Universities of Shandong/College of Life Science, Shandong Normal University, Jinan 250014, P.R. China; Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R. China; Correspondence FENG Xian-zhong, Tel: +86-431-85655051, Fax: +86-431-85542298Plant height is one of the most important traits in soybean. The semi-dwarf soybean cultivars could improve the ability of lodging resistance to obtain higher yield. To broaden the dwarfism germplasm resources in soybean, 44 dwarf mutants were identified from a gamma rays mutagenized M2 population. Two of these mutants, Gmdwf1 (Glycine max dwarf 1) and Gmdwf2 (Glycine max dwarf 2), were investigated in this study. Genetic analysis showed that both mutants were inherited in a recessive manner and their mutated regions were delimited to a 2.610-Mb region on chromosome 1 by preliminary mapping. Further fine mapping study proved that the two mutants had a common deletion region of 1.552 Mb in the target region, which was located in a novel locus site without being reported previously. The dwarfism of Gmdwf1 could not be rescued by gibberellin (GA) and brassinolide (BR) treatments, which indicated that the biosynthesis of these hormones was not deficient in Gmdwf1.http://www.sciencedirect.com/science/article/pii/S2095311915613120soybeandwarf mutantmappingBRGA |
| spellingShingle | Wen CHENG Jin-shan GAO Xing-xing FENG Qun SHAO Su-xin YANG Xian-zhong FENG Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean Journal of Integrative Agriculture soybean dwarf mutant mapping BR GA |
| title | Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean |
| title_full | Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean |
| title_fullStr | Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean |
| title_full_unstemmed | Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean |
| title_short | Characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean |
| title_sort | characterization of dwarf mutants and molecular mapping of a dwarf locus in soybean |
| topic | soybean dwarf mutant mapping BR GA |
| url | http://www.sciencedirect.com/science/article/pii/S2095311915613120 |
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