Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica
Introduction: Perfluoroalkyl and poly-fluoroalkyl substances (PFASs) are widely used in industrial and consumer products. Due to their environmental persistence and bioaccumulation, PFASs can be found in the blood of humans and wild animals all over the world. Various fluorinated alternatives such a...
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| Format: | Article |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2023.1073461/full |
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| author | Wenqi Cao Wenqi Cao Katharine Horzmann Bettina Schemera Myra Petrofski Trisha Kendall Jennifer Spooner Patricia E. Rynders John L. VandeBerg Xu Wang Xu Wang Xu Wang |
| author_facet | Wenqi Cao Wenqi Cao Katharine Horzmann Bettina Schemera Myra Petrofski Trisha Kendall Jennifer Spooner Patricia E. Rynders John L. VandeBerg Xu Wang Xu Wang Xu Wang |
| author_sort | Wenqi Cao |
| collection | DOAJ |
| description | Introduction: Perfluoroalkyl and poly-fluoroalkyl substances (PFASs) are widely used in industrial and consumer products. Due to their environmental persistence and bioaccumulation, PFASs can be found in the blood of humans and wild animals all over the world. Various fluorinated alternatives such as GenX have been developed to replace the long-chain PFASs, but there is limited information about their potential toxicity.Methods:The current study developed blood culture protocols to assess the response to toxic compounds in the marsupial, Monodelphis domestica. After whole-blood culture conditions were tested and optimized, changes in gene expression in response to PFOA and GenX treatment were assessed.Results: More than 10,000 genes were expressed in the blood transcriptomes with and without treatment. Both PFOA and GenX treatment led to significant changes in the whole blood culture transcriptomes. A total of 578 and 148 differentially expressed genes (DEGs) were detected in the PFOA and GenX treatment groups, 32 of which overlapped. Pathway enrichment analysis revealed that DEGs involved in developmental processes were upregulated after PFOA exposure, while those enriched for metabolic and immune system processes were downregulated. GenX exposure upregulated genes associated with fatty acid transport pathways and inflammatory processes, which is consistent with previous studies using rodent models.Discussion: To our knowledge, this study is the first to investigate the effect of PFASs in a marsupial model. The findings provide supportive evidence for significant transcriptomic alterations, suggesting that this mammalian model may provide a mechanism for exploring the potential toxicity of PFOA and GenX. |
| first_indexed | 2024-04-10T15:04:38Z |
| format | Article |
| id | doaj.art-d8f45ccfa2694853a446fde3a6c37bff |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-10T15:04:38Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-d8f45ccfa2694853a446fde3a6c37bff2023-02-15T05:33:32ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-02-011410.3389/fgene.2023.10734611073461Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domesticaWenqi Cao0Wenqi Cao1Katharine Horzmann2Bettina Schemera3Myra Petrofski4Trisha Kendall5Jennifer Spooner6Patricia E. Rynders7John L. VandeBerg8Xu Wang9Xu Wang10Xu Wang11Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesAlabama Agricultural Experiment Station, Auburn University Center for Advanced Science, Innovation, and Commerce, Auburn, AL, United StatesDepartment of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesDivision of Laboratory Animal Health, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesDivision of Laboratory Animal Health, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesDivision of Laboratory Animal Health, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesDivision of Laboratory Animal Health, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesDivision of Laboratory Animal Health, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesDepartment of Human Genetics, School of Medicine, South Texas Diabetes and Obesity Institute, The University of Texas Rio Grande Valley, Brownsville, TX, United StatesDepartment of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL, United StatesAlabama Agricultural Experiment Station, Auburn University Center for Advanced Science, Innovation, and Commerce, Auburn, AL, United StatesHudsonAlpha Institute for Biotechnology, Huntsville, AL, United StatesIntroduction: Perfluoroalkyl and poly-fluoroalkyl substances (PFASs) are widely used in industrial and consumer products. Due to their environmental persistence and bioaccumulation, PFASs can be found in the blood of humans and wild animals all over the world. Various fluorinated alternatives such as GenX have been developed to replace the long-chain PFASs, but there is limited information about their potential toxicity.Methods:The current study developed blood culture protocols to assess the response to toxic compounds in the marsupial, Monodelphis domestica. After whole-blood culture conditions were tested and optimized, changes in gene expression in response to PFOA and GenX treatment were assessed.Results: More than 10,000 genes were expressed in the blood transcriptomes with and without treatment. Both PFOA and GenX treatment led to significant changes in the whole blood culture transcriptomes. A total of 578 and 148 differentially expressed genes (DEGs) were detected in the PFOA and GenX treatment groups, 32 of which overlapped. Pathway enrichment analysis revealed that DEGs involved in developmental processes were upregulated after PFOA exposure, while those enriched for metabolic and immune system processes were downregulated. GenX exposure upregulated genes associated with fatty acid transport pathways and inflammatory processes, which is consistent with previous studies using rodent models.Discussion: To our knowledge, this study is the first to investigate the effect of PFASs in a marsupial model. The findings provide supportive evidence for significant transcriptomic alterations, suggesting that this mammalian model may provide a mechanism for exploring the potential toxicity of PFOA and GenX.https://www.frontiersin.org/articles/10.3389/fgene.2023.1073461/fullper-and polyfluoroalkyl substances (PFAS)laboratory opossumlong-chain fatty acid transportinflammatory responsedevelopmental processestoxicology |
| spellingShingle | Wenqi Cao Wenqi Cao Katharine Horzmann Bettina Schemera Myra Petrofski Trisha Kendall Jennifer Spooner Patricia E. Rynders John L. VandeBerg Xu Wang Xu Wang Xu Wang Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica Frontiers in Genetics per-and polyfluoroalkyl substances (PFAS) laboratory opossum long-chain fatty acid transport inflammatory response developmental processes toxicology |
| title | Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica |
| title_full | Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica |
| title_fullStr | Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica |
| title_full_unstemmed | Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica |
| title_short | Blood transcriptome responses to PFOA and GenX treatment in the marsupial biomedical model Monodelphis domestica |
| title_sort | blood transcriptome responses to pfoa and genx treatment in the marsupial biomedical model monodelphis domestica |
| topic | per-and polyfluoroalkyl substances (PFAS) laboratory opossum long-chain fatty acid transport inflammatory response developmental processes toxicology |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2023.1073461/full |
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