Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S]
Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as difference...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-06-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520310105 |
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| author | Hiten D. Mistry Lesia O. Kurlak Yosef T. Mansour Line Zurkinden Markus G. Mohaupt Geneviève Escher |
| author_facet | Hiten D. Mistry Lesia O. Kurlak Yosef T. Mansour Line Zurkinden Markus G. Mohaupt Geneviève Escher |
| author_sort | Hiten D. Mistry |
| collection | DOAJ |
| description | Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1 binding protein (AIBP). Total, HDL-, and ABCA1-mediated cholesterol effluxes were performed with maternal and fetal plasma from women with preeclampsia and normotensive controls (both n = 17). apoA1 and apoE were quantified by chemiluminescence, and 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localization of CYP27A1, AIBP, apoA1, apoE, and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in preeclampsia (by 10–20%), but ABCA1-mediated efflux was decreased (by 20–35%; P < 0.05). Maternal and fetal apoE concentrations were higher in preeclampsia. Fetal plasma 27-OHC levels were decreased in preeclamptic samples (P< 0.05). Placental protein expression of both CYP27A1 and AIBP were localized around fetal vessels and significantly increased in preeclampsia (P = 0.04). Placental 27-OHC concentrations were also raised in preeclampsia (P < 0.05). Increased HDL-mediated cholesterol efflux capacity and placental CYP27A1/27-OHC could be a rescue mechanism in preeclampsia, to remove cholesterol from cells to limit lipid peroxidation and increase placental angiogenesis. |
| first_indexed | 2024-12-21T13:04:19Z |
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| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:04:19Z |
| publishDate | 2017-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-d8f7e66350144491ab19250f45cf463b2022-12-21T19:03:06ZengElsevierJournal of Lipid Research0022-22752017-06-0158611861195Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S]Hiten D. Mistry0Lesia O. Kurlak1Yosef T. Mansour2Line Zurkinden3Markus G. Mohaupt4Geneviève Escher5To whom correspondence should be addressed.; Department of Nephrology, Hypertension, Clinical Pharmacology, and Clinical Research, University of Bern, Bern, Switzerland; Division of Child Health, Obstetrics, and Gynecology, School of Medicine, University of Nottingham, Nottingham, United KingdomDivision of Child Health, Obstetrics, and Gynecology, School of Medicine, University of Nottingham, Nottingham, United KingdomDivision of Women's Health, Women's Health Academic Centre, King's College London, London, United KingdomDepartment of Nephrology, Hypertension, Clinical Pharmacology, and Clinical Research, University of Bern, Bern, SwitzerlandDepartment of Nephrology, Hypertension, Clinical Pharmacology, and Clinical Research, University of Bern, Bern, SwitzerlandDepartment of Nephrology, Hypertension, Clinical Pharmacology, and Clinical Research, University of Bern, Bern, SwitzerlandPreeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1 binding protein (AIBP). Total, HDL-, and ABCA1-mediated cholesterol effluxes were performed with maternal and fetal plasma from women with preeclampsia and normotensive controls (both n = 17). apoA1 and apoE were quantified by chemiluminescence, and 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localization of CYP27A1, AIBP, apoA1, apoE, and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in preeclampsia (by 10–20%), but ABCA1-mediated efflux was decreased (by 20–35%; P < 0.05). Maternal and fetal apoE concentrations were higher in preeclampsia. Fetal plasma 27-OHC levels were decreased in preeclamptic samples (P< 0.05). Placental protein expression of both CYP27A1 and AIBP were localized around fetal vessels and significantly increased in preeclampsia (P = 0.04). Placental 27-OHC concentrations were also raised in preeclampsia (P < 0.05). Increased HDL-mediated cholesterol efflux capacity and placental CYP27A1/27-OHC could be a rescue mechanism in preeclampsia, to remove cholesterol from cells to limit lipid peroxidation and increase placental angiogenesis.http://www.sciencedirect.com/science/article/pii/S0022227520310105lipid/efflux27-hydroxycholesterolapolipoproteinspregnancyhypertensionsterol 27-hydroxylase |
| spellingShingle | Hiten D. Mistry Lesia O. Kurlak Yosef T. Mansour Line Zurkinden Markus G. Mohaupt Geneviève Escher Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S] Journal of Lipid Research lipid/efflux 27-hydroxycholesterol apolipoproteins pregnancy hypertension sterol 27-hydroxylase |
| title | Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S] |
| title_full | Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S] |
| title_fullStr | Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S] |
| title_full_unstemmed | Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S] |
| title_short | Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia[S] |
| title_sort | increased maternal and fetal cholesterol efflux capacity and placental cyp27a1 expression in preeclampsia s |
| topic | lipid/efflux 27-hydroxycholesterol apolipoproteins pregnancy hypertension sterol 27-hydroxylase |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520310105 |
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