Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, Kenya
INTRODUCTION: During the storage of transfusion blood, it may undergo a series of cellular changes that in speculation could be the reason behind the risk of using prolonged stored blood. It´s important therefore to monitor the cellular changes that may reduce its survival and function. The objectiv...
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| Format: | Article |
| Language: | English |
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The Pan African Medical Journal
2021-03-01
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| Series: | The Pan African Medical Journal |
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| Online Access: |
https://www.panafrican-med-journal.com/content/article/38/280/pdf/280.pdf
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| _version_ | 1819125204594458624 |
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| author | Phidelis Maruti Marabi Stanslaus Kiilu Musyoki Angela Amayo |
| author_facet | Phidelis Maruti Marabi Stanslaus Kiilu Musyoki Angela Amayo |
| author_sort | Phidelis Maruti Marabi |
| collection | DOAJ |
| description | INTRODUCTION: During the storage of transfusion blood, it may undergo a series of cellular changes that in speculation could be the reason behind the risk of using prolonged stored blood. It´s important therefore to monitor the cellular changes that may reduce its survival and function. The objective was to assess the cellular changes in whole blood stored for transfusion at Bungoma county referral hospital. METHODS: A single center, prospective and observational study design involving 20 randomly selected donor blood units in citrate phosphate dextrose adenine (CPDA-1) anticoagulant was employed, cellular changes were evaluated for 35 days. The changes were tested using the Celtac F Haematology analyzer. Statistical Analysis of variance was employed in the descriptive statistics. All the investigation was executed using statistical package for social sciences (SPSS V.23). Results were regarded as significant at P=0.05. Results were presented in tables and charts. RESULTS: At the end of the 35 days blood storage at blood bank conditions , WBC, RBC, platelets counts and MCHC decreased significantly (P=0.0001, =0.0182, 0.0001,=0.0035). The MCV, HCT and MCH increased significantly (P=0.0003, =0.0115) while HGB had insignificant variance (P =0.4185). CONCLUSION: Platelets, WBC, RBC counts, and indices are significantly altered in stored blood especially when stored over two weeks based on most of the cellular components analyzed in this study. The study, therefore, recommends the utilization of fresh blood to avoid the adverse outcome of cellular changes of reserved blood. |
| first_indexed | 2024-12-22T07:36:25Z |
| format | Article |
| id | doaj.art-d8fa9955c8784c6c910267b3a6497c47 |
| institution | Directory Open Access Journal |
| issn | 1937-8688 1937-8688 |
| language | English |
| last_indexed | 2024-12-22T07:36:25Z |
| publishDate | 2021-03-01 |
| publisher | The Pan African Medical Journal |
| record_format | Article |
| series | The Pan African Medical Journal |
| spelling | doaj.art-d8fa9955c8784c6c910267b3a6497c472022-12-21T18:33:53ZengThe Pan African Medical JournalThe Pan African Medical Journal1937-86881937-86882021-03-013828010.11604/pamj.2021.38.280.2232722327Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, KenyaPhidelis Maruti Marabi0Stanslaus Kiilu Musyoki1Angela Amayo2 Bungoma County Referral Hospital, Bungoma, Kenya School of Health Sciences, Kisii University, Kisii, Kenya Department of Human Pathology, University of Nairobi, Nairobi, Kenya INTRODUCTION: During the storage of transfusion blood, it may undergo a series of cellular changes that in speculation could be the reason behind the risk of using prolonged stored blood. It´s important therefore to monitor the cellular changes that may reduce its survival and function. The objective was to assess the cellular changes in whole blood stored for transfusion at Bungoma county referral hospital. METHODS: A single center, prospective and observational study design involving 20 randomly selected donor blood units in citrate phosphate dextrose adenine (CPDA-1) anticoagulant was employed, cellular changes were evaluated for 35 days. The changes were tested using the Celtac F Haematology analyzer. Statistical Analysis of variance was employed in the descriptive statistics. All the investigation was executed using statistical package for social sciences (SPSS V.23). Results were regarded as significant at P=0.05. Results were presented in tables and charts. RESULTS: At the end of the 35 days blood storage at blood bank conditions , WBC, RBC, platelets counts and MCHC decreased significantly (P=0.0001, =0.0182, 0.0001,=0.0035). The MCV, HCT and MCH increased significantly (P=0.0003, =0.0115) while HGB had insignificant variance (P =0.4185). CONCLUSION: Platelets, WBC, RBC counts, and indices are significantly altered in stored blood especially when stored over two weeks based on most of the cellular components analyzed in this study. The study, therefore, recommends the utilization of fresh blood to avoid the adverse outcome of cellular changes of reserved blood. https://www.panafrican-med-journal.com/content/article/38/280/pdf/280.pdf blood transfusioncellular changesstoragekenya |
| spellingShingle | Phidelis Maruti Marabi Stanslaus Kiilu Musyoki Angela Amayo Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, Kenya The Pan African Medical Journal blood transfusion cellular changes storage kenya |
| title | Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, Kenya |
| title_full | Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, Kenya |
| title_fullStr | Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, Kenya |
| title_full_unstemmed | Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, Kenya |
| title_short | Evaluation of cellular changes in blood stored for transfusion at Bungoma County Referral Hospital, Kenya |
| title_sort | evaluation of cellular changes in blood stored for transfusion at bungoma county referral hospital kenya |
| topic | blood transfusion cellular changes storage kenya |
| url |
https://www.panafrican-med-journal.com/content/article/38/280/pdf/280.pdf
|
| work_keys_str_mv | AT phidelismarutimarabi evaluationofcellularchangesinbloodstoredfortransfusionatbungomacountyreferralhospitalkenya AT stanslauskiilumusyoki evaluationofcellularchangesinbloodstoredfortransfusionatbungomacountyreferralhospitalkenya AT angelaamayo evaluationofcellularchangesinbloodstoredfortransfusionatbungomacountyreferralhospitalkenya |