Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3

Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3

Objective To investigate the effects of CTGF on proliferation and migration of SKOV3 and the underlying mechanism. Methods SKOV3 cells were treated with recombinant human CTGF (rhCTGF).The proliferation of SKOV3 was detected by CCK-8 assay and colony formation assay. The migration of SKOV3 was det...

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Bibliographic Details
Main Author: DONG Ling-ling, GAO Yun, NIU Guo-yu
Format: Article
Language:zho
Published: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-02-01
Series:Jichu yixue yu linchuang
Subjects:
ctgf|ovarian cancer cell|proliferation|migration|mechanism
Online Access:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200215.pdf
Description
Summary:Objective To investigate the effects of CTGF on proliferation and migration of SKOV3 and the underlying mechanism. Methods SKOV3 cells were treated with recombinant human CTGF (rhCTGF).The proliferation of SKOV3 was detected by CCK-8 assay and colony formation assay. The migration of SKOV3 was detected by wound healing assay.The expression of EMT-related proteins was detected by Western blot. Results After rhCTGF treatment, the proliferation rate of SKOV3 was increased, the colony formation and migzation were enhanced (P＜0.05).The expression of E-cadherin was decreased and the expression of vimentin was increased, which were related proteins of EMT(P＜0.05). Conclusions CTGF can promote proliferation, growth and metastasis of SKOV3, and that may contribute to the regulation of epithelium-mesenchymal transformation (EMT).
ISSN:1001-6325

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