Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3
Objective To investigate the effects of CTGF on proliferation and migration of SKOV3 and the underlying mechanism. Methods SKOV3 cells were treated with recombinant human CTGF (rhCTGF).The proliferation of SKOV3 was detected by CCK-8 assay and colony formation assay. The migration of SKOV3 was det...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200215.pdf |
| _version_ | 1797365818804142080 |
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| author | DONG Ling-ling, GAO Yun, NIU Guo-yu |
| author_facet | DONG Ling-ling, GAO Yun, NIU Guo-yu |
| author_sort | DONG Ling-ling, GAO Yun, NIU Guo-yu |
| collection | DOAJ |
| description | Objective To investigate the effects of CTGF on proliferation and migration of SKOV3 and the underlying mechanism. Methods SKOV3 cells were treated with recombinant human CTGF (rhCTGF).The proliferation of SKOV3 was detected by CCK-8 assay and colony formation assay. The migration of SKOV3 was detected by wound healing assay.The expression of EMT-related proteins was detected by Western blot. Results After rhCTGF treatment, the proliferation rate of SKOV3 was increased, the colony formation and migzation were enhanced (P＜0.05).The expression of E-cadherin was decreased and the expression of vimentin was increased, which were related proteins of EMT(P＜0.05). Conclusions CTGF can promote proliferation, growth and metastasis of SKOV3, and that may contribute to the regulation of epithelium-mesenchymal transformation (EMT). |
| first_indexed | 2024-03-08T16:55:21Z |
| format | Article |
| id | doaj.art-d8fb3d608b5a43f08272f61c485cad4b |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:21Z |
| publishDate | 2021-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-d8fb3d608b5a43f08272f61c485cad4b2024-01-05T03:04:29ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-02-01412193196Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3DONG Ling-ling, GAO Yun, NIU Guo-yu01. Tumor Center, Weifang Hospital of Traditional Chinese Medicine; ;2. School of Public Health and Management, Weifang Medical University, Weifang 261000, ChinaObjective To investigate the effects of CTGF on proliferation and migration of SKOV3 and the underlying mechanism. Methods SKOV3 cells were treated with recombinant human CTGF (rhCTGF).The proliferation of SKOV3 was detected by CCK-8 assay and colony formation assay. The migration of SKOV3 was detected by wound healing assay.The expression of EMT-related proteins was detected by Western blot. Results After rhCTGF treatment, the proliferation rate of SKOV3 was increased, the colony formation and migzation were enhanced (P＜0.05).The expression of E-cadherin was decreased and the expression of vimentin was increased, which were related proteins of EMT(P＜0.05). Conclusions CTGF can promote proliferation, growth and metastasis of SKOV3, and that may contribute to the regulation of epithelium-mesenchymal transformation (EMT).http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200215.pdfctgf|ovarian cancer cell|proliferation|migration|mechanism |
| spellingShingle | DONG Ling-ling, GAO Yun, NIU Guo-yu Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3 Jichu yixue yu linchuang ctgf|ovarian cancer cell|proliferation|migration|mechanism |
| title | Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3 |
| title_full | Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3 |
| title_fullStr | Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3 |
| title_full_unstemmed | Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3 |
| title_short | Connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line SKOV3 |
| title_sort | connective tissue growth factor promotes the proliferation and migration of human ovarian cancer cell line skov3 |
| topic | ctgf|ovarian cancer cell|proliferation|migration|mechanism |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200215.pdf |
| work_keys_str_mv | AT donglinglinggaoyunniuguoyu connectivetissuegrowthfactorpromotestheproliferationandmigrationofhumanovariancancercelllineskov3 |