Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki
Hybrid sturgeon is the main species of sturgeon cultured in China, with the advantages of a fast growth rate, early sexual maturity, fertile offspring, and more stable genetic traits. In May 2021, a large number of deaths characterized by superficial hemorrhage and liver damage occurred in a sturgeo...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1029692/full |
| _version_ | 1811229326638579712 |
|---|---|
| author | Mengwei Zhang Mengwei Zhang Mingyang Xue Zidong Xiao Wei Liu Nan Jiang Yan Meng Yuding Fan Xiaoling Liu Yong Zhou |
| author_facet | Mengwei Zhang Mengwei Zhang Mingyang Xue Zidong Xiao Wei Liu Nan Jiang Yan Meng Yuding Fan Xiaoling Liu Yong Zhou |
| author_sort | Mengwei Zhang |
| collection | DOAJ |
| description | Hybrid sturgeon is the main species of sturgeon cultured in China, with the advantages of a fast growth rate, early sexual maturity, fertile offspring, and more stable genetic traits. In May 2021, a large number of deaths characterized by superficial hemorrhage and liver damage occurred in a sturgeon farm in Yichang, Hubei Province, which posed a significant risk to hybrid sturgeon captive breeding. We isolated a pathogenic bacterium named D-59 from the diseased sturgeon with apparent symptoms. The pathogen was identified as Staphylococcus sciuri using 16S rRNA gene phylogenetic analysis combined with biochemical identification. Regression experiments showed that D-59 exhibited clinical signs similar to those of diseased sturgeon in the farm after intraperitoneal injection into hybrid sturgeon. High-throughput sequencing of gut microbes in D-59-infected sturgeon showed that the number of gut microbial species decreased in infected sturgeon, the number of some intestinal commensal bacteria decreased, and the balance of the intestinal microorganisms was disrupted. Histopathological sections indicated many inflammatory cells, congestion, and even necrosis in the tissue of diseased sturgeon. Analysis of blood indexes revealed an increase in the proportion of mononuclear cells and a decrease in the proportion of lymphocytes in the peripheral blood of diseased sturgeon. Significantly elevated serum levels of aspartate aminotransferase and alanine aminotransferase, whereas alkaline phosphatase, total protein, albumin, and globulin were decreased in diseased sturgeon. Antimicrobial susceptibility tests demonstrated that D-59 is susceptible to florfenicol, enrofloxacin, and neomycin sulfate. This study aimed to highlight the dangers of Staphylococcus sciuri infection during hybrid sturgeon culture and to provide recommendations for diagnosis and treatment. |
| first_indexed | 2024-04-12T10:12:46Z |
| format | Article |
| id | doaj.art-d8fe974523824b39ae4ef9cc446c1991 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T10:12:46Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d8fe974523824b39ae4ef9cc446c19912022-12-22T03:37:17ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-10-011210.3389/fcimb.2022.10296921029692Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrenckiMengwei Zhang0Mengwei Zhang1Mingyang Xue2Zidong Xiao3Wei Liu4Nan Jiang5Yan Meng6Yuding Fan7Xiaoling Liu8Yong Zhou9Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaDepartment of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, ChinaHybrid sturgeon is the main species of sturgeon cultured in China, with the advantages of a fast growth rate, early sexual maturity, fertile offspring, and more stable genetic traits. In May 2021, a large number of deaths characterized by superficial hemorrhage and liver damage occurred in a sturgeon farm in Yichang, Hubei Province, which posed a significant risk to hybrid sturgeon captive breeding. We isolated a pathogenic bacterium named D-59 from the diseased sturgeon with apparent symptoms. The pathogen was identified as Staphylococcus sciuri using 16S rRNA gene phylogenetic analysis combined with biochemical identification. Regression experiments showed that D-59 exhibited clinical signs similar to those of diseased sturgeon in the farm after intraperitoneal injection into hybrid sturgeon. High-throughput sequencing of gut microbes in D-59-infected sturgeon showed that the number of gut microbial species decreased in infected sturgeon, the number of some intestinal commensal bacteria decreased, and the balance of the intestinal microorganisms was disrupted. Histopathological sections indicated many inflammatory cells, congestion, and even necrosis in the tissue of diseased sturgeon. Analysis of blood indexes revealed an increase in the proportion of mononuclear cells and a decrease in the proportion of lymphocytes in the peripheral blood of diseased sturgeon. Significantly elevated serum levels of aspartate aminotransferase and alanine aminotransferase, whereas alkaline phosphatase, total protein, albumin, and globulin were decreased in diseased sturgeon. Antimicrobial susceptibility tests demonstrated that D-59 is susceptible to florfenicol, enrofloxacin, and neomycin sulfate. This study aimed to highlight the dangers of Staphylococcus sciuri infection during hybrid sturgeon culture and to provide recommendations for diagnosis and treatment.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1029692/fullhybrid sturgeonstaphylococcus sciurintestinal microorganismshematological parametershistopatholog |
| spellingShingle | Mengwei Zhang Mengwei Zhang Mingyang Xue Zidong Xiao Wei Liu Nan Jiang Yan Meng Yuding Fan Xiaoling Liu Yong Zhou Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki Frontiers in Cellular and Infection Microbiology hybrid sturgeon staphylococcus sciur intestinal microorganisms hematological parameters histopatholog |
| title | Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki |
| title_full | Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki |
| title_fullStr | Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki |
| title_full_unstemmed | Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki |
| title_short | Staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii × acipenser schrencki |
| title_sort | staphylococcus sciuri causes disease and pathological changes in hybrid sturgeon acipenser baerii acipenser schrencki |
| topic | hybrid sturgeon staphylococcus sciur intestinal microorganisms hematological parameters histopatholog |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1029692/full |
| work_keys_str_mv | AT mengweizhang staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT mengweizhang staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT mingyangxue staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT zidongxiao staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT weiliu staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT nanjiang staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT yanmeng staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT yudingfan staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT xiaolingliu staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki AT yongzhou staphylococcussciuricausesdiseaseandpathologicalchangesinhybridsturgeonacipenserbaeriiacipenserschrencki |