Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea Genotypes
Mycorrhiza-resistant and non-nodulating pea mutants provide a model system for identifying common genes regulated during the early events in mycorrhiza and nodule establishment. Inoculation of pea roots with Glomus mosseae or Rhizobium leguminosarum can induce overex-pression of seven defense-relate...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1999-11-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.11.976 |
| _version_ | 1811291355444412416 |
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| author | Juan M. Ruiz-Lozano Hélène Roussel Silvio Gianinazzi Vivienne Gianinazzi-Pearson |
| author_facet | Juan M. Ruiz-Lozano Hélène Roussel Silvio Gianinazzi Vivienne Gianinazzi-Pearson |
| author_sort | Juan M. Ruiz-Lozano |
| collection | DOAJ |
| description | Mycorrhiza-resistant and non-nodulating pea mutants provide a model system for identifying common genes regulated during the early events in mycorrhiza and nodule establishment. Inoculation of pea roots with Glomus mosseae or Rhizobium leguminosarum can induce overex-pression of seven defense-related genes (pI 206, pI 49, pI 176, PR 10, basic A1-chitinase, transcinnamic acid 4-hydroxylase, chalcone isomerase), depending on the plant genotype and the time point of interaction between the plant and the microsymbiont. Expression of the pI 206 gene is closely correlated with appressorium formation by the mycorrhizal fungus on both mutant and wild-type pea roots. The gene is also induced by the pathogen Aphanomyces euteiches. Transcript accumulation was higher in mutant than in wild-type genotypes for five and six of the studied genes during early stages of root interactions with G. mosseae and R. leguminosarum, respectively, and this is discussed in relation to the symbiotic-defective phenotype of Myc-1Nod¯ pea. The early induction of similar plant defense genes in response to arbuscular mycorrhizal fungi and rhizobia reinforces the hypothesis of common regulatory steps in both root symbioses. |
| first_indexed | 2024-04-13T04:28:05Z |
| format | Article |
| id | doaj.art-d8ff00b42f1849cf8398e604f64277f6 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:28:05Z |
| publishDate | 1999-11-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-d8ff00b42f1849cf8398e604f64277f62022-12-22T03:02:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-11-01121197698410.1094/MPMI.1999.12.11.976Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea GenotypesJuan M. Ruiz-LozanoHélène RousselSilvio GianinazziVivienne Gianinazzi-PearsonMycorrhiza-resistant and non-nodulating pea mutants provide a model system for identifying common genes regulated during the early events in mycorrhiza and nodule establishment. Inoculation of pea roots with Glomus mosseae or Rhizobium leguminosarum can induce overex-pression of seven defense-related genes (pI 206, pI 49, pI 176, PR 10, basic A1-chitinase, transcinnamic acid 4-hydroxylase, chalcone isomerase), depending on the plant genotype and the time point of interaction between the plant and the microsymbiont. Expression of the pI 206 gene is closely correlated with appressorium formation by the mycorrhizal fungus on both mutant and wild-type pea roots. The gene is also induced by the pathogen Aphanomyces euteiches. Transcript accumulation was higher in mutant than in wild-type genotypes for five and six of the studied genes during early stages of root interactions with G. mosseae and R. leguminosarum, respectively, and this is discussed in relation to the symbiotic-defective phenotype of Myc-1Nod¯ pea. The early induction of similar plant defense genes in response to arbuscular mycorrhizal fungi and rhizobia reinforces the hypothesis of common regulatory steps in both root symbioses.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.11.976gene expressionnodule symbiosesPisum sativum |
| spellingShingle | Juan M. Ruiz-Lozano Hélène Roussel Silvio Gianinazzi Vivienne Gianinazzi-Pearson Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea Genotypes Molecular Plant-Microbe Interactions gene expression nodule symbioses Pisum sativum |
| title | Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea Genotypes |
| title_full | Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea Genotypes |
| title_fullStr | Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea Genotypes |
| title_full_unstemmed | Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea Genotypes |
| title_short | Defense Genes Are Differentially Induced by a Mycorrhizal Fungus and Rhizobium sp. in Wild-Type and Symbiosis-Defective Pea Genotypes |
| title_sort | defense genes are differentially induced by a mycorrhizal fungus and rhizobium sp in wild type and symbiosis defective pea genotypes |
| topic | gene expression nodule symbioses Pisum sativum |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.11.976 |
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