Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior
Exosomes have been related to various disorders, but their study in relation to ocular pathologies has been limited. In this article, we analyze exosomes produced by corneal stromal cells from healthy individuals and from patients with keratoconus. The proteomic study allowed for the identification...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/10/2348 |
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| author | Víctor Lozano Carla Martín Noelia Blanco Ignacio Alcalde Luis Fernandez-Vega Cueto Jesús Merayo-Lloves Luis M. Quirós |
| author_facet | Víctor Lozano Carla Martín Noelia Blanco Ignacio Alcalde Luis Fernandez-Vega Cueto Jesús Merayo-Lloves Luis M. Quirós |
| author_sort | Víctor Lozano |
| collection | DOAJ |
| description | Exosomes have been related to various disorders, but their study in relation to ocular pathologies has been limited. In this article, we analyze exosomes produced by corneal stromal cells from healthy individuals and from patients with keratoconus. The proteomic study allowed for the identification of 14 new proteins with altered expression, related to molecules previously associated with the pathology. miRNA analysis detected 16 altered species, including miR-184, responsible for familial severe keratoconus. The prediction of its potential biological targets identified 1121 genes, including some related to this pathology. Exosomes produced by keratoconic cells induced a marked increase in the migration of stromal cells and corneal epithelium, while those produced by healthy cells had no effect on stromal cells. Both types of nanovesicles reduced the proliferation of stromal and corneal cells, but those produced by healthy cells had less effect. Exosomes produced by healthy cells had concentration-dependent effects on the transcription of genes encoding proteoglycans by keratoconus cells, with a relative normalization observed at concentrations of 240 µg/mL. These results show the alteration of stromal exosomes in keratoconus and suggest an influence on the development of the pathology, although the use of healthy exosomes could also have therapeutic potential. |
| first_indexed | 2024-03-09T20:40:19Z |
| format | Article |
| id | doaj.art-d9019921d85f46f1817aa6fa2c6be46c |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T20:40:19Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-d9019921d85f46f1817aa6fa2c6be46c2023-11-23T23:01:40ZengMDPI AGBiomedicines2227-90592022-09-011010234810.3390/biomedicines10102348Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular BehaviorVíctor Lozano0Carla Martín1Noelia Blanco2Ignacio Alcalde3Luis Fernandez-Vega Cueto4Jesús Merayo-Lloves5Luis M. Quirós6Department of Functional Biology, University of Oviedo, 33006 Oviedo, SpainDepartment of Functional Biology, University of Oviedo, 33006 Oviedo, SpainDepartment of Functional Biology, University of Oviedo, 33006 Oviedo, SpainInstituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, University of Oviedo, 33012 Oviedo, SpainInstituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, University of Oviedo, 33012 Oviedo, SpainInstituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, University of Oviedo, 33012 Oviedo, SpainDepartment of Functional Biology, University of Oviedo, 33006 Oviedo, SpainExosomes have been related to various disorders, but their study in relation to ocular pathologies has been limited. In this article, we analyze exosomes produced by corneal stromal cells from healthy individuals and from patients with keratoconus. The proteomic study allowed for the identification of 14 new proteins with altered expression, related to molecules previously associated with the pathology. miRNA analysis detected 16 altered species, including miR-184, responsible for familial severe keratoconus. The prediction of its potential biological targets identified 1121 genes, including some related to this pathology. Exosomes produced by keratoconic cells induced a marked increase in the migration of stromal cells and corneal epithelium, while those produced by healthy cells had no effect on stromal cells. Both types of nanovesicles reduced the proliferation of stromal and corneal cells, but those produced by healthy cells had less effect. Exosomes produced by healthy cells had concentration-dependent effects on the transcription of genes encoding proteoglycans by keratoconus cells, with a relative normalization observed at concentrations of 240 µg/mL. These results show the alteration of stromal exosomes in keratoconus and suggest an influence on the development of the pathology, although the use of healthy exosomes could also have therapeutic potential.https://www.mdpi.com/2227-9059/10/10/2348exosomeskeratoconuscorneal stromaproteomicsmiRNA |
| spellingShingle | Víctor Lozano Carla Martín Noelia Blanco Ignacio Alcalde Luis Fernandez-Vega Cueto Jesús Merayo-Lloves Luis M. Quirós Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior Biomedicines exosomes keratoconus corneal stroma proteomics miRNA |
| title | Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior |
| title_full | Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior |
| title_fullStr | Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior |
| title_full_unstemmed | Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior |
| title_short | Exosomes Released by Corneal Stromal Cells Show Molecular Alterations in Keratoconus Patients and Induce Different Cellular Behavior |
| title_sort | exosomes released by corneal stromal cells show molecular alterations in keratoconus patients and induce different cellular behavior |
| topic | exosomes keratoconus corneal stroma proteomics miRNA |
| url | https://www.mdpi.com/2227-9059/10/10/2348 |
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