HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women
Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate...
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MDPI AG
2021-11-01
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| Series: | Molecules |
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| author | Aniceta A. Mikulska Dorota Filipowicz Franciszek K. Główka Ewelina Szczepanek-Parulska Marek Ruchała Michał Bartecki Marta Karaźniewicz-Łada |
| author_facet | Aniceta A. Mikulska Dorota Filipowicz Franciszek K. Główka Ewelina Szczepanek-Parulska Marek Ruchała Michał Bartecki Marta Karaźniewicz-Łada |
| author_sort | Aniceta A. Mikulska |
| collection | DOAJ |
| description | Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate solution was added to the urine sample to convert the total free iodine to iodide. Chromatographic separation was achieved in a Pursuit XRs C8 column. The mobile phase consisted of acetonitrile and a water phase containing 18-crown-6-ether, octylamine and sodium dihydrogen phosphate. Validation parameters, such as accuracy, precision, limits of detection and quantification, linearity and stability, were determined. Urinary samples from pregnant women were used to complete the validation and confirm the method’s applicability. In the studied population of 93 pregnant women, the median UIC was lower in the group without iodine supplementation (117 µg/L, confidence interval (%CI): 95; 138) than in the supplement group (133 µg/L, %CI: 109; 157). In conclusion, the newly established ion-pair HPLC–UV method was adequately precise, accurate and fulfilled validation the criteria for analyzing compounds in biological fluids. The method is less complicated and expensive than other frequently used assays and permits the identification of the iodine-deficient subjects. |
| first_indexed | 2024-03-10T05:15:11Z |
| format | Article |
| id | doaj.art-d9025bc5e8134fccbdbfa4097c7f8c02 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:15:11Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d9025bc5e8134fccbdbfa4097c7f8c022023-11-23T00:33:55ZengMDPI AGMolecules1420-30492021-11-012622679710.3390/molecules26226797HPLC Analysis of the Urinary Iodine Concentration in Pregnant WomenAniceta A. Mikulska0Dorota Filipowicz1Franciszek K. Główka2Ewelina Szczepanek-Parulska3Marek Ruchała4Michał Bartecki5Marta Karaźniewicz-Łada6Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 60-781 Poznań, PolandDepartment of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-356 Poznań, PolandDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 60-781 Poznań, PolandDepartment of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-356 Poznań, PolandDepartment of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-356 Poznań, PolandDepartment of Pediatric Cardiology, Poznan University of Medical Science, 60-572 Poznań, PolandDepartment of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences, 60-781 Poznań, PolandIodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC–UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate solution was added to the urine sample to convert the total free iodine to iodide. Chromatographic separation was achieved in a Pursuit XRs C8 column. The mobile phase consisted of acetonitrile and a water phase containing 18-crown-6-ether, octylamine and sodium dihydrogen phosphate. Validation parameters, such as accuracy, precision, limits of detection and quantification, linearity and stability, were determined. Urinary samples from pregnant women were used to complete the validation and confirm the method’s applicability. In the studied population of 93 pregnant women, the median UIC was lower in the group without iodine supplementation (117 µg/L, confidence interval (%CI): 95; 138) than in the supplement group (133 µg/L, %CI: 109; 157). In conclusion, the newly established ion-pair HPLC–UV method was adequately precise, accurate and fulfilled validation the criteria for analyzing compounds in biological fluids. The method is less complicated and expensive than other frequently used assays and permits the identification of the iodine-deficient subjects.https://www.mdpi.com/1420-3049/26/22/6797ion-pair HPLC–UV methodvalidationin vivo applicationiodine supplementationpregnancy |
| spellingShingle | Aniceta A. Mikulska Dorota Filipowicz Franciszek K. Główka Ewelina Szczepanek-Parulska Marek Ruchała Michał Bartecki Marta Karaźniewicz-Łada HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women Molecules ion-pair HPLC–UV method validation in vivo application iodine supplementation pregnancy |
| title | HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women |
| title_full | HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women |
| title_fullStr | HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women |
| title_full_unstemmed | HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women |
| title_short | HPLC Analysis of the Urinary Iodine Concentration in Pregnant Women |
| title_sort | hplc analysis of the urinary iodine concentration in pregnant women |
| topic | ion-pair HPLC–UV method validation in vivo application iodine supplementation pregnancy |
| url | https://www.mdpi.com/1420-3049/26/22/6797 |
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