Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene Synthase
Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. <i>Mentha</i> plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However,...
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MDPI AG
2021-04-01
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| author | Zequn Chen Kelly J. Vining Xiwu Qi Xu Yu Ying Zheng Zhiqi Liu Hailing Fang Li Li Yang Bai Chengyuan Liang Weilin Li Bernd Markus Lange |
| author_facet | Zequn Chen Kelly J. Vining Xiwu Qi Xu Yu Ying Zheng Zhiqi Liu Hailing Fang Li Li Yang Bai Chengyuan Liang Weilin Li Bernd Markus Lange |
| author_sort | Zequn Chen |
| collection | DOAJ |
| description | Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. <i>Mentha</i> plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in <i>Mentha</i> plants. In this work, we genome-widely identified and analyzed the <i>TPS</i> gene family in <i>Mentha longifolia</i>, a model plant for functional genomic research in the genus <i>Mentha</i>. A total of 63 <i>TPS</i> genes were identified in the <i>M. longifolia</i> genome sequence assembly, which could be divided into six subfamilies. The <i>TPS-b</i> subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in <i>Mentha</i> plants. The <i>TPS-e</i> subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 <i>TPS</i> genes could be mapped to nine scaffolds of the <i>M. longifolia</i> genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of <i>TPS</i> genes in <i>M. longifolia</i>. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in <i>M. longifolia</i> TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that <i>M. longifolia TPSs</i> were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, <i>MlongTPS29</i>, which belongs to the <i>TPS-b</i> subfamily. <i>MlongTPS29</i> could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus <i>Mentha</i>. This study provides useful information for the biosynthesis of terpenoids in the genus <i>Mentha</i>. |
| first_indexed | 2024-03-10T12:40:02Z |
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| spelling | doaj.art-d906e18dc835484bb9a06fb5019a3d072023-11-21T13:59:00ZengMDPI AGGenes2073-44252021-04-0112451810.3390/genes12040518Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene SynthaseZequn Chen0Kelly J. Vining1Xiwu Qi2Xu Yu3Ying Zheng4Zhiqi Liu5Hailing Fang6Li Li7Yang Bai8Chengyuan Liang9Weilin Li10Bernd Markus Lange11Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaDepartment of Horticulture, Oregon State University, Corvallis, OR 97331, USAInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaZhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Hangzhou 311300, ChinaNanjing Foreign Language School, Nanjing 210008, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, ChinaCollege of Forest, Nanjing Forestry University, Nanjing 210037, ChinaInstitute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA 99164, USATerpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. <i>Mentha</i> plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in <i>Mentha</i> plants. In this work, we genome-widely identified and analyzed the <i>TPS</i> gene family in <i>Mentha longifolia</i>, a model plant for functional genomic research in the genus <i>Mentha</i>. A total of 63 <i>TPS</i> genes were identified in the <i>M. longifolia</i> genome sequence assembly, which could be divided into six subfamilies. The <i>TPS-b</i> subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in <i>Mentha</i> plants. The <i>TPS-e</i> subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 <i>TPS</i> genes could be mapped to nine scaffolds of the <i>M. longifolia</i> genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of <i>TPS</i> genes in <i>M. longifolia</i>. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in <i>M. longifolia</i> TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that <i>M. longifolia TPSs</i> were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, <i>MlongTPS29</i>, which belongs to the <i>TPS-b</i> subfamily. <i>MlongTPS29</i> could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus <i>Mentha</i>. This study provides useful information for the biosynthesis of terpenoids in the genus <i>Mentha</i>.https://www.mdpi.com/2073-4425/12/4/518<i>Mentha longifolia</i>terpene synthaseterpenoidslimonene synthase |
| spellingShingle | Zequn Chen Kelly J. Vining Xiwu Qi Xu Yu Ying Zheng Zhiqi Liu Hailing Fang Li Li Yang Bai Chengyuan Liang Weilin Li Bernd Markus Lange Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene Synthase Genes <i>Mentha longifolia</i> terpene synthase terpenoids limonene synthase |
| title | Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene Synthase |
| title_full | Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene Synthase |
| title_fullStr | Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene Synthase |
| title_full_unstemmed | Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene Synthase |
| title_short | Genome-Wide Analysis of Terpene Synthase Gene Family in <em>Mentha longifolia</em> and Catalytic Activity Analysis of a Single Terpene Synthase |
| title_sort | genome wide analysis of terpene synthase gene family in em mentha longifolia em and catalytic activity analysis of a single terpene synthase |
| topic | <i>Mentha longifolia</i> terpene synthase terpenoids limonene synthase |
| url | https://www.mdpi.com/2073-4425/12/4/518 |
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