Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting
Layered Double Hydroxides (LDHs) are inorganic compounds of relevance to various domains, where their surface reactivity and/or intercalation capacities can be advantageously exploited for the retention/release of ionic and molecular species. In this study, we have explored specifically the applicab...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/6/734 |
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| author | Hyoung-Jun Kim Prescillia Lagarrigue Jae-Min Oh Jérémy Soulié Fabrice Salles Sophie Cazalbou Christophe Drouet |
| author_facet | Hyoung-Jun Kim Prescillia Lagarrigue Jae-Min Oh Jérémy Soulié Fabrice Salles Sophie Cazalbou Christophe Drouet |
| author_sort | Hyoung-Jun Kim |
| collection | DOAJ |
| description | Layered Double Hydroxides (LDHs) are inorganic compounds of relevance to various domains, where their surface reactivity and/or intercalation capacities can be advantageously exploited for the retention/release of ionic and molecular species. In this study, we have explored specifically the applicability in the field of bone regeneration of one LDH composition, denoted “MgFeCO<sub>3</sub>”, of which components are already present in vivo, so as to convey a biocompatibility character. The propensity to be used as a bone substitute depends, however, on their ability to allow the fabrication of 3D constructs able to be implanted in bone sites. In this work, we display two appealing approaches for the processing of MgFeCO<sub>3</sub> LDH particles to prepare (<i>i</i>) porous 3D scaffolds by freeze-casting, involving an alginate biopolymeric matrix, and (<i>ii</i>) pure MgFeCO<sub>3</sub> LDH monoliths by Spark Plasma Sintering (SPS) at low temperature. We then explored the capacity of such LDH particles or monoliths to interact quantitatively with molecular moieties/drugs in view of their local release. The experimental data were complemented by computational chemistry calculations (Monte Carlo) to examine in more detail the mineral–organic interactions at play. Finally, preliminary in vitro tests on osteoblastic MG63 cells confirmed the high biocompatible character of this LDH composition. It was confirmed that (<i>i</i>) thermodynamically metastable LDH could be successfully consolidated into a monolith through SPS, (<i>ii</i>) the LDH particles could be incorporated into a polymer matrix through freeze casting, and (<i>iii</i>) the LDH in the consolidated monolith could incorporate and release drug molecules in a controlled manner. In other words, our results indicate that the MgFeCO<sub>3</sub> LDH (pyroaurite structure) may be seen as a new promising compound for the setup of bone substitute biomaterials with tailorable drug delivery capacity, including for personalized medicine. |
| first_indexed | 2024-03-11T02:46:14Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T02:46:14Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-d90ab5d3b3d64eb5b84e4eb26beac6552023-11-18T09:22:00ZengMDPI AGBioengineering2306-53542023-06-0110673410.3390/bioengineering10060734Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-CastingHyoung-Jun Kim0Prescillia Lagarrigue1Jae-Min Oh2Jérémy Soulié3Fabrice Salles4Sophie Cazalbou5Christophe Drouet6CIRIMAT, Université de Toulouse, CNRS, Toulouse INP, 31030 Toulouse, FranceCIRIMAT, Université de Toulouse, CNRS, Toulouse INP, 31030 Toulouse, FranceResearch Institute, National Cancer Center, Goyang 10408, Republic of KoreaCIRIMAT, Université de Toulouse, CNRS, Toulouse INP, 31030 Toulouse, FranceInstitute Charles Gerhardt des Matériaux (ICGM), Université de Montpellier, CNRS, ENSCM, 34090 Montpellier, FranceCIRIMAT, Université de Toulouse, CNRS, Toulouse INP, 31030 Toulouse, FranceCIRIMAT, Université de Toulouse, CNRS, Toulouse INP, 31030 Toulouse, FranceLayered Double Hydroxides (LDHs) are inorganic compounds of relevance to various domains, where their surface reactivity and/or intercalation capacities can be advantageously exploited for the retention/release of ionic and molecular species. In this study, we have explored specifically the applicability in the field of bone regeneration of one LDH composition, denoted “MgFeCO<sub>3</sub>”, of which components are already present in vivo, so as to convey a biocompatibility character. The propensity to be used as a bone substitute depends, however, on their ability to allow the fabrication of 3D constructs able to be implanted in bone sites. In this work, we display two appealing approaches for the processing of MgFeCO<sub>3</sub> LDH particles to prepare (<i>i</i>) porous 3D scaffolds by freeze-casting, involving an alginate biopolymeric matrix, and (<i>ii</i>) pure MgFeCO<sub>3</sub> LDH monoliths by Spark Plasma Sintering (SPS) at low temperature. We then explored the capacity of such LDH particles or monoliths to interact quantitatively with molecular moieties/drugs in view of their local release. The experimental data were complemented by computational chemistry calculations (Monte Carlo) to examine in more detail the mineral–organic interactions at play. Finally, preliminary in vitro tests on osteoblastic MG63 cells confirmed the high biocompatible character of this LDH composition. It was confirmed that (<i>i</i>) thermodynamically metastable LDH could be successfully consolidated into a monolith through SPS, (<i>ii</i>) the LDH particles could be incorporated into a polymer matrix through freeze casting, and (<i>iii</i>) the LDH in the consolidated monolith could incorporate and release drug molecules in a controlled manner. In other words, our results indicate that the MgFeCO<sub>3</sub> LDH (pyroaurite structure) may be seen as a new promising compound for the setup of bone substitute biomaterials with tailorable drug delivery capacity, including for personalized medicine.https://www.mdpi.com/2306-5354/10/6/734LDHporosity3D bone scaffoldscold sinteringfreeze-castingcomputational chemistry |
| spellingShingle | Hyoung-Jun Kim Prescillia Lagarrigue Jae-Min Oh Jérémy Soulié Fabrice Salles Sophie Cazalbou Christophe Drouet Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting Bioengineering LDH porosity 3D bone scaffolds cold sintering freeze-casting computational chemistry |
| title | Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
| title_full | Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
| title_fullStr | Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
| title_full_unstemmed | Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
| title_short | Biocompatible MgFeCO<sub>3</sub> Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting |
| title_sort | biocompatible mgfeco sub 3 sub layered double hydroxide ldh for bone regeneration low temperature processing through cold sintering and freeze casting |
| topic | LDH porosity 3D bone scaffolds cold sintering freeze-casting computational chemistry |
| url | https://www.mdpi.com/2306-5354/10/6/734 |
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