Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing
Abstract Background The bactericidal effect of disinfectants against biofilms is essential to reduce potential endoscopy-related infections caused by contamination. Here, we investigated the bactericidal effect of a high-level disinfectant, peracetic acid (PAA), against Staphylococcus aureus and Pse...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Antimicrobial Resistance and Infection Control |
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| Online Access: | http://link.springer.com/article/10.1186/s13756-017-0281-1 |
| _version_ | 1818216568728846336 |
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| author | T. Chino Y. Nukui Y. Morishita K. Moriya |
| author_facet | T. Chino Y. Nukui Y. Morishita K. Moriya |
| author_sort | T. Chino |
| collection | DOAJ |
| description | Abstract Background The bactericidal effect of disinfectants against biofilms is essential to reduce potential endoscopy-related infections caused by contamination. Here, we investigated the bactericidal effect of a high-level disinfectant, peracetic acid (PAA), against Staphylococcus aureus and Pseudomonas aeruginosa biofilm models in vitro. Methods S. aureus and P. aeruginosa biofilms were cultured at 35 °C for 7 days with catheter tubes. The following high-level disinfectants (HLDs) were tested: 0.3% PAA, 0.55% ortho-phthalaldehyde (OPA), and 2.0% alkaline-buffered glutaraldehyde (GA). Biofilms were exposed to these agents for 1–60 min and observed after 5 min and 30 min by transmission and scanning electron microscopy. A Student’s t test was performed to compare the exposure time required for bactericidal effectiveness of the disinfectants. Results PAA and GA were active within 1 min and 5 min, respectively, against S. aureus and P. aeruginosa biofilms. OPA took longer than 10 min and 30 min to act against S. aureus and P. aeruginosa biofilms, respectively (p < 0.01). Treatment with PAA elicited changes in cell shape after 5 min and structural damage after 30 min. Conclusions Amongst the HLDs investigated, PAA elicited the most rapid bactericidal effects against both biofilms. Additionally, treatment with PAA induced morphological alterations in the in vitro biofilm models, suggesting that PAA exerts fast-acting bactericidal effects against biofilms associated with endoscopy-related infections. These findings indicate that the exposure time for bactericidal effectiveness of HLDs for endoscope reprocessing in healthcare settings should be reconsidered. |
| first_indexed | 2024-12-12T06:54:03Z |
| format | Article |
| id | doaj.art-d90b686d17024078b47c03e57130352f |
| institution | Directory Open Access Journal |
| issn | 2047-2994 |
| language | English |
| last_indexed | 2024-12-12T06:54:03Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Antimicrobial Resistance and Infection Control |
| spelling | doaj.art-d90b686d17024078b47c03e57130352f2022-12-22T00:34:00ZengBMCAntimicrobial Resistance and Infection Control2047-29942017-12-01611710.1186/s13756-017-0281-1Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubingT. Chino0Y. Nukui1Y. Morishita2K. Moriya3Department of Infection Control and Prevention, Medical Hospital, Tokyo Medical and Dental UniversityDepartment of Infection Control and Prevention, Medical Hospital, Tokyo Medical and Dental UniversityDepartment of Molecular Pathology, Graduate School of Medicine, The University of TokyoDepartment of Infection Control and Prevention, The University of Tokyo HospitalAbstract Background The bactericidal effect of disinfectants against biofilms is essential to reduce potential endoscopy-related infections caused by contamination. Here, we investigated the bactericidal effect of a high-level disinfectant, peracetic acid (PAA), against Staphylococcus aureus and Pseudomonas aeruginosa biofilm models in vitro. Methods S. aureus and P. aeruginosa biofilms were cultured at 35 °C for 7 days with catheter tubes. The following high-level disinfectants (HLDs) were tested: 0.3% PAA, 0.55% ortho-phthalaldehyde (OPA), and 2.0% alkaline-buffered glutaraldehyde (GA). Biofilms were exposed to these agents for 1–60 min and observed after 5 min and 30 min by transmission and scanning electron microscopy. A Student’s t test was performed to compare the exposure time required for bactericidal effectiveness of the disinfectants. Results PAA and GA were active within 1 min and 5 min, respectively, against S. aureus and P. aeruginosa biofilms. OPA took longer than 10 min and 30 min to act against S. aureus and P. aeruginosa biofilms, respectively (p < 0.01). Treatment with PAA elicited changes in cell shape after 5 min and structural damage after 30 min. Conclusions Amongst the HLDs investigated, PAA elicited the most rapid bactericidal effects against both biofilms. Additionally, treatment with PAA induced morphological alterations in the in vitro biofilm models, suggesting that PAA exerts fast-acting bactericidal effects against biofilms associated with endoscopy-related infections. These findings indicate that the exposure time for bactericidal effectiveness of HLDs for endoscope reprocessing in healthcare settings should be reconsidered.http://link.springer.com/article/10.1186/s13756-017-0281-1Peracetic acidStaphylococcus aureus biofilmPseudomonas aeruginosa biofilmBactericidal effectivenessExposure timeElectron microscopy |
| spellingShingle | T. Chino Y. Nukui Y. Morishita K. Moriya Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing Antimicrobial Resistance and Infection Control Peracetic acid Staphylococcus aureus biofilm Pseudomonas aeruginosa biofilm Bactericidal effectiveness Exposure time Electron microscopy |
| title | Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing |
| title_full | Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing |
| title_fullStr | Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing |
| title_full_unstemmed | Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing |
| title_short | Morphological bactericidal fast-acting effects of peracetic acid, a high-level disinfectant, against Staphylococcus aureus and Pseudomonas aeruginosa biofilms in tubing |
| title_sort | morphological bactericidal fast acting effects of peracetic acid a high level disinfectant against staphylococcus aureus and pseudomonas aeruginosa biofilms in tubing |
| topic | Peracetic acid Staphylococcus aureus biofilm Pseudomonas aeruginosa biofilm Bactericidal effectiveness Exposure time Electron microscopy |
| url | http://link.springer.com/article/10.1186/s13756-017-0281-1 |
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