Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells
Adipose tissue may represent a potential source of adult stem cells for tissue engineering applications in veterinary medicine. It can be obtained in large quantities, under local anesthesia, and with minimal discomfort. In this study, canine adipose tissue was obtained by biopsy from subcutaneous a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2010-03-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368909X481764 |
| _version_ | 1818328688773562368 |
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| author | N. M. Vieira V. Brandalise E. Zucconi M. Secco B. E. Strauss M. Zatz Ph.D. |
| author_facet | N. M. Vieira V. Brandalise E. Zucconi M. Secco B. E. Strauss M. Zatz Ph.D. |
| author_sort | N. M. Vieira |
| collection | DOAJ |
| description | Adipose tissue may represent a potential source of adult stem cells for tissue engineering applications in veterinary medicine. It can be obtained in large quantities, under local anesthesia, and with minimal discomfort. In this study, canine adipose tissue was obtained by biopsy from subcutaneous adipose tissue or by suction-assisted lipectomy (i.e., liposuction). Adipose tissue was processed to obtain a fibroblast-like population of cells similar to human adipose-derived stem cells (hASCs). These canine adipose-derived stem cells (cASCs) can be maintained in vitro for extended periods with stable population doubling and low levels of senescence. Immunofluorescence and flow cytometry show that the majority of cASCs are of mesodermal or mesenchymal origin. cASCs are able to differentiate in vitro into adipogenic, chondrogenic, myogenic, and osteogenic cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirate, canine adipose tissue may also contain multipotent cells and represent an important stem cell source both for veterinary cell therapy as well as preclinical studies. |
| first_indexed | 2024-12-13T12:36:09Z |
| format | Article |
| id | doaj.art-d90c2cfd0db344158b21f20e5aba532c |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T12:36:09Z |
| publishDate | 2010-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-d90c2cfd0db344158b21f20e5aba532c2022-12-21T23:45:52ZengSAGE PublishingCell Transplantation0963-68971555-38922010-03-011910.3727/096368909X481764Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem CellsN. M. Vieira0V. Brandalise1E. Zucconi2M. Secco3B. E. Strauss4M. Zatz Ph.D.5Human Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, BrazilHuman Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, BrazilHuman Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, BrazilHuman Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, BrazilViral Vector Group, Heart Institute, InCor, University of São Paulo, São Paulo, BrazilHuman Genome Research Center, Biosciences Institute, University of São Paulo, São Paulo, BrazilAdipose tissue may represent a potential source of adult stem cells for tissue engineering applications in veterinary medicine. It can be obtained in large quantities, under local anesthesia, and with minimal discomfort. In this study, canine adipose tissue was obtained by biopsy from subcutaneous adipose tissue or by suction-assisted lipectomy (i.e., liposuction). Adipose tissue was processed to obtain a fibroblast-like population of cells similar to human adipose-derived stem cells (hASCs). These canine adipose-derived stem cells (cASCs) can be maintained in vitro for extended periods with stable population doubling and low levels of senescence. Immunofluorescence and flow cytometry show that the majority of cASCs are of mesodermal or mesenchymal origin. cASCs are able to differentiate in vitro into adipogenic, chondrogenic, myogenic, and osteogenic cells in the presence of lineage-specific induction factors. In conclusion, like human lipoaspirate, canine adipose tissue may also contain multipotent cells and represent an important stem cell source both for veterinary cell therapy as well as preclinical studies.https://doi.org/10.3727/096368909X481764 |
| spellingShingle | N. M. Vieira V. Brandalise E. Zucconi M. Secco B. E. Strauss M. Zatz Ph.D. Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells Cell Transplantation |
| title | Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells |
| title_full | Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells |
| title_fullStr | Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells |
| title_full_unstemmed | Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells |
| title_short | Isolation, Characterization, and Differentiation Potential of Canine Adipose-Derived Stem Cells |
| title_sort | isolation characterization and differentiation potential of canine adipose derived stem cells |
| url | https://doi.org/10.3727/096368909X481764 |
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