THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT
Objective: To investigate the effect of dexamethasone on spermatogonium and sertoli cell of ipsilateral testis in unilateral testicular torsion strain wistar rat. Material & Method: Experimental study with post-test only control group design. The present study was conducted on 30 Wistar male ra...
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| Format: | Article |
| Language: | English |
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IKATAN AHLI UROLOGI INDONESIA
2018-07-01
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| Series: | Jurnal Urologi Indonesia |
| Subjects: | |
| Online Access: | http://juri.urologi.or.id/juri/article/view/466 |
| _version_ | 1818791576907808768 |
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| author | Ferdyan Rachmat Efendi Johan Renaldo Tarmono Djojodimedjo |
| author_facet | Ferdyan Rachmat Efendi Johan Renaldo Tarmono Djojodimedjo |
| author_sort | Ferdyan Rachmat Efendi |
| collection | DOAJ |
| description | Objective: To investigate the effect of dexamethasone on spermatogonium and sertoli cell of ipsilateral testis in unilateral testicular torsion strain wistar rat. Material & Method: Experimental study with post-test only control group design. The present study was conducted on 30 Wistar male rats aged 10 – 12 weeks grouped into 5 groups. Group I was the normal/sham operation group (KN), group II was left testicular torsion for 4 hours group and followed by manual detorsion (K1), group III was left testicular torsion for 10 hours group and followed by manual detorsion (K2), group IV was left testicular torsion for 4 hours group and given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual etorsion (D1), and group V was left testicular torsion for 10 hours group and given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual detorsion. All rats had left orchidectomy 4 hours after detorsion. The number of spermatogonium and sertoli cells were counted in histological seminiferous tubular testis that have obtained Haematoxylin Eosin staining. Data were analyzed by ANNOVA followed by Post Hoc Tukey for spermatogonium and Kruskal Wallis followed by Mann Whitney test for sertoli cell. Differences were considered significant at p <0.05. Results: There was significant difference in the mean number of spermatogonium between K1 & D1 group. Otherwise, there was no significant difference in the mean number of spermatogonium between K2 & D2. There was significant difference in the mean number of Sertoli cells between K1 & D1 group, likewise that between K2 & D2 group. Conclusion: These results suggest that dexamethasone has protective effect in spermatogonium and sertoli cell in testicular torsion for 4 hours. |
| first_indexed | 2024-12-18T15:13:33Z |
| format | Article |
| id | doaj.art-d90d8e0adb3f49faa866782c48d9148b |
| institution | Directory Open Access Journal |
| issn | 0853-442X 2355-1402 |
| language | English |
| last_indexed | 2024-12-18T15:13:33Z |
| publishDate | 2018-07-01 |
| publisher | IKATAN AHLI UROLOGI INDONESIA |
| record_format | Article |
| series | Jurnal Urologi Indonesia |
| spelling | doaj.art-d90d8e0adb3f49faa866782c48d9148b2022-12-21T21:03:36ZengIKATAN AHLI UROLOGI INDONESIAJurnal Urologi Indonesia0853-442X2355-14022018-07-0125210.32421/juri.v25i2.466466THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RATFerdyan Rachmat Efendi0Johan Renaldo1Tarmono Djojodimedjo2Department of Urology, Faculty of Medicine/Airlangga University, Soetomo Hospital, Surabaya.Department of Urology, Faculty of Medicine/Airlangga University, Soetomo Hospital, Surabaya.Department of Urology, Faculty of Medicine/Airlangga University, Soetomo Hospital, Surabaya.Objective: To investigate the effect of dexamethasone on spermatogonium and sertoli cell of ipsilateral testis in unilateral testicular torsion strain wistar rat. Material & Method: Experimental study with post-test only control group design. The present study was conducted on 30 Wistar male rats aged 10 – 12 weeks grouped into 5 groups. Group I was the normal/sham operation group (KN), group II was left testicular torsion for 4 hours group and followed by manual detorsion (K1), group III was left testicular torsion for 10 hours group and followed by manual detorsion (K2), group IV was left testicular torsion for 4 hours group and given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual etorsion (D1), and group V was left testicular torsion for 10 hours group and given dexamethasone 10 mg/kg body weight subcutaneously 30 minutes before manual detorsion. All rats had left orchidectomy 4 hours after detorsion. The number of spermatogonium and sertoli cells were counted in histological seminiferous tubular testis that have obtained Haematoxylin Eosin staining. Data were analyzed by ANNOVA followed by Post Hoc Tukey for spermatogonium and Kruskal Wallis followed by Mann Whitney test for sertoli cell. Differences were considered significant at p <0.05. Results: There was significant difference in the mean number of spermatogonium between K1 & D1 group. Otherwise, there was no significant difference in the mean number of spermatogonium between K2 & D2. There was significant difference in the mean number of Sertoli cells between K1 & D1 group, likewise that between K2 & D2 group. Conclusion: These results suggest that dexamethasone has protective effect in spermatogonium and sertoli cell in testicular torsion for 4 hours.http://juri.urologi.or.id/juri/article/view/466dexamethasonespermatogoniumsertoli cellstesticular torsion |
| spellingShingle | Ferdyan Rachmat Efendi Johan Renaldo Tarmono Djojodimedjo THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT Jurnal Urologi Indonesia dexamethasone spermatogonium sertoli cells testicular torsion |
| title | THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT |
| title_full | THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT |
| title_fullStr | THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT |
| title_full_unstemmed | THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT |
| title_short | THE EFFECT OF DEXAMETHASONE ON SPERMATOGONIUM AND SERTOLI CELL OF IPSILATERAL TESTIS IN UNILATERAL TESTICULAR TORSION WISTAR RAT |
| title_sort | effect of dexamethasone on spermatogonium and sertoli cell of ipsilateral testis in unilateral testicular torsion wistar rat |
| topic | dexamethasone spermatogonium sertoli cells testicular torsion |
| url | http://juri.urologi.or.id/juri/article/view/466 |
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