Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe
Tao Zhang,1 Fang Tian,2 Lin Long,1 Jianbo Liu,3 Xiaochun Wu4 1Department of Clinical Laboratory, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, China; 2Department of Clinical Laboratory, Zaozhuang Municipal Maternal and Child Health Hospital, Zaozhuang, China; 3College of...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-08-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/diagnosis-of-rubella-virus-using-antigen-conjugated-aupt-nanorods-as-n-peer-reviewed-article-IJN |
| _version_ | 1818694419486867456 |
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| author | Zhang T Tian F Long L Liu JB Wu XC |
| author_facet | Zhang T Tian F Long L Liu JB Wu XC |
| author_sort | Zhang T |
| collection | DOAJ |
| description | Tao Zhang,1 Fang Tian,2 Lin Long,1 Jianbo Liu,3 Xiaochun Wu4 1Department of Clinical Laboratory, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, China; 2Department of Clinical Laboratory, Zaozhuang Municipal Maternal and Child Health Hospital, Zaozhuang, China; 3College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, China; 4CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, China Background: As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools. Purpose: Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen. Method: Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus. Results: Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity. Conclusion: Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions. Keywords: gold nanorods, nanozyme, antigen conjugation, rubella virus, diagnosis |
| first_indexed | 2024-12-17T13:29:17Z |
| format | Article |
| id | doaj.art-d90e1c163b6d4b4ba6707933ae08a63d |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T13:29:17Z |
| publishDate | 2018-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-d90e1c163b6d4b4ba6707933ae08a63d2022-12-21T21:46:38ZengDove Medical PressInternational Journal of Nanomedicine1178-20132018-08-01Volume 134795480540027Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probeZhang TTian FLong LLiu JBWu XCTao Zhang,1 Fang Tian,2 Lin Long,1 Jianbo Liu,3 Xiaochun Wu4 1Department of Clinical Laboratory, Zaozhuang Municipal Center for Disease Control and Prevention, Zaozhuang, China; 2Department of Clinical Laboratory, Zaozhuang Municipal Maternal and Child Health Hospital, Zaozhuang, China; 3College of Opto-electronic Engineering, Zaozhuang University, Zaozhuang, China; 4CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, China Background: As nanotechnology advances rapidly, the nanozymes which could replace protein enzymes in bioanalytical assays bring a new opportunity to the development of simple and sensitive diagnostic tools. Purpose: Antibody-conjugated Au-Pt core/shell nanorods (Au@Pt NRs) have been used for nanozyme probes for diagnosis of rubella virus. Au@Pt NRs, prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase (HRP) in conjugation of antigen. Method: Using a capture enzyme-linked immunosorbant assay for the determination of rubella virus. Results: Compared with antibody-conjugated HRP, the antigen-conjugated Au@Pt NRs are more stable and more robust, but less expensive. Based on the enhanced catalytic properties of this nanozyme probe, it was found that the antigen-conjugated Au@Pt NRs-based enzyme-linked immunosorbant assay exhibited good sensitivity. Conclusion: Our results indicate that these antigen-conjugated Au@Pt NRs represent a suitable nanozyme probe for future clinical applications under various conditions. Keywords: gold nanorods, nanozyme, antigen conjugation, rubella virus, diagnosishttps://www.dovepress.com/diagnosis-of-rubella-virus-using-antigen-conjugated-aupt-nanorods-as-n-peer-reviewed-article-IJNGold nanorodsPlatinumnanozymeEnzyme-linked immunosorbent assay(ELISA)Virus diagnosis |
| spellingShingle | Zhang T Tian F Long L Liu JB Wu XC Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe International Journal of Nanomedicine Gold nanorods Platinum nanozyme Enzyme-linked immunosorbent assay(ELISA) Virus diagnosis |
| title | Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe |
| title_full | Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe |
| title_fullStr | Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe |
| title_full_unstemmed | Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe |
| title_short | Diagnosis of rubella virus using antigen-conjugated Au@Pt nanorods as nanozyme probe |
| title_sort | diagnosis of rubella virus using antigen conjugated au pt nanorods as nanozyme probe |
| topic | Gold nanorods Platinum nanozyme Enzyme-linked immunosorbent assay(ELISA) Virus diagnosis |
| url | https://www.dovepress.com/diagnosis-of-rubella-virus-using-antigen-conjugated-aupt-nanorods-as-n-peer-reviewed-article-IJN |
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