A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model
Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by the production of inflammatory factors. In order to overcome the side effects of currently used anti-inflammatory drugs, several attempts have been made to identify natural products capable of relieving RA sympto...
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MDPI AG
2020-05-01
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| author | Valentina Parisi Antonio Vassallo Claudio Pisano Giacomo Signorino Francesco Cardile Milena Sorrentino Fabiana Colelli Alessandra Fucci Egildo Luca D’Andrea Nunziatina De Tommasi Alessandra Braca Marinella De Leo |
| author_facet | Valentina Parisi Antonio Vassallo Claudio Pisano Giacomo Signorino Francesco Cardile Milena Sorrentino Fabiana Colelli Alessandra Fucci Egildo Luca D’Andrea Nunziatina De Tommasi Alessandra Braca Marinella De Leo |
| author_sort | Valentina Parisi |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by the production of inflammatory factors. In order to overcome the side effects of currently used anti-inflammatory drugs, several attempts have been made to identify natural products capable of relieving RA symptoms. In this work, a herbal preparation consisting of propolis, pomegranate peel, and Aglianico grape pomace (PPP) extracts (4:1:1) was designed and evaluated for its effect on a murine collagen-induced arthritis (CIA) model. Firstly, the chemical contents of four different Italian propolis collected in the Campania region (Italy) were here reported for the first time. LC-MS analyses showed the presence of 38 constituents, identified in all propolis extracts, belonging to flavonoids and phenolic acids classes. The Pietradefusi extract was the richest one and thus was selected to design the PPP preparation for the in vivo assay. Our results highlight the impact of PPP on RA onset and progression. By using in vivo CIA models, the treatment with PPP resulted in a delayed onset of the disease and alleviated the severity of the clinical symptoms. Furthermore, we demonstrated that early PPP treatment was associated with a reduction in serum levels of IL-17, IL-1b, and IL-17–triggering cytokines. |
| first_indexed | 2024-03-10T19:55:55Z |
| format | Article |
| id | doaj.art-d90f401c39134fd490402678ade12513 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T19:55:55Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d90f401c39134fd490402678ade125132023-11-20T00:01:39ZengMDPI AGMolecules1420-30492020-05-01259225510.3390/molecules25092255A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis ModelValentina Parisi0Antonio Vassallo1Claudio Pisano2Giacomo Signorino3Francesco Cardile4Milena Sorrentino5Fabiana Colelli6Alessandra Fucci7Egildo Luca D’Andrea8Nunziatina De Tommasi9Alessandra Braca10Marinella De Leo11Dipartimento di Farmacia, Università degli Studi di Salerno, 84084 Fisciano (SA), ItalyDipartimento di Scienze, Università della Basilicata, 85100 Potenza, ItalyBiogem, Research Institute “G. Salvatore”, 83031 Ariano Irpino (AV), ItalyBiogem, Research Institute “G. Salvatore”, 83031 Ariano Irpino (AV), ItalyBiogem, Research Institute “G. Salvatore”, 83031 Ariano Irpino (AV), ItalyBiogem, Research Institute “G. Salvatore”, 83031 Ariano Irpino (AV), ItalyBiogem, Research Institute “G. Salvatore”, 83031 Ariano Irpino (AV), ItalyBiogem, Research Institute “G. Salvatore”, 83031 Ariano Irpino (AV), ItalyBiogem, Research Institute “G. Salvatore”, 83031 Ariano Irpino (AV), ItalyDipartimento di Farmacia, Università degli Studi di Salerno, 84084 Fisciano (SA), ItalyDipartimento di Farmacia, Università di Pisa, 56126 Pisa, ItalyDipartimento di Farmacia, Università di Pisa, 56126 Pisa, ItalyRheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease characterized by the production of inflammatory factors. In order to overcome the side effects of currently used anti-inflammatory drugs, several attempts have been made to identify natural products capable of relieving RA symptoms. In this work, a herbal preparation consisting of propolis, pomegranate peel, and Aglianico grape pomace (PPP) extracts (4:1:1) was designed and evaluated for its effect on a murine collagen-induced arthritis (CIA) model. Firstly, the chemical contents of four different Italian propolis collected in the Campania region (Italy) were here reported for the first time. LC-MS analyses showed the presence of 38 constituents, identified in all propolis extracts, belonging to flavonoids and phenolic acids classes. The Pietradefusi extract was the richest one and thus was selected to design the PPP preparation for the in vivo assay. Our results highlight the impact of PPP on RA onset and progression. By using in vivo CIA models, the treatment with PPP resulted in a delayed onset of the disease and alleviated the severity of the clinical symptoms. Furthermore, we demonstrated that early PPP treatment was associated with a reduction in serum levels of IL-17, IL-1b, and IL-17–triggering cytokines.https://www.mdpi.com/1420-3049/25/9/2255propolispomegranateAglianico grape pomaceherbal preparationLC-ESI-MS/MSrheumatoid arthritis |
| spellingShingle | Valentina Parisi Antonio Vassallo Claudio Pisano Giacomo Signorino Francesco Cardile Milena Sorrentino Fabiana Colelli Alessandra Fucci Egildo Luca D’Andrea Nunziatina De Tommasi Alessandra Braca Marinella De Leo A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model Molecules propolis pomegranate Aglianico grape pomace herbal preparation LC-ESI-MS/MS rheumatoid arthritis |
| title | A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model |
| title_full | A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model |
| title_fullStr | A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model |
| title_full_unstemmed | A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model |
| title_short | A Herbal Mixture from Propolis, Pomegranate, and Grape Pomace Endowed with Anti-Inflammatory Activity in an In Vivo Rheumatoid Arthritis Model |
| title_sort | herbal mixture from propolis pomegranate and grape pomace endowed with anti inflammatory activity in an in vivo rheumatoid arthritis model |
| topic | propolis pomegranate Aglianico grape pomace herbal preparation LC-ESI-MS/MS rheumatoid arthritis |
| url | https://www.mdpi.com/1420-3049/25/9/2255 |
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