Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster Analysis
Marine symbiotic actinomycetes play a key role in drug development and their ecological niches can influence a variety of natural product biosynthesis, providing potential defensive benefits. In this study, we report the whole-genome sequence analysis of marine gastropod mollusk <i>Planaxis<...
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MDPI AG
2023-02-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/11/2/396 |
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| author | Ganesan Govindarajan Ziwei Yao Zhenbin Zhou Xiaohong Zheng Junying Ma Pachaiyappan Saravana Kumar Jianhua Ju Changli Sun |
| author_facet | Ganesan Govindarajan Ziwei Yao Zhenbin Zhou Xiaohong Zheng Junying Ma Pachaiyappan Saravana Kumar Jianhua Ju Changli Sun |
| author_sort | Ganesan Govindarajan |
| collection | DOAJ |
| description | Marine symbiotic actinomycetes play a key role in drug development and their ecological niches can influence a variety of natural product biosynthesis, providing potential defensive benefits. In this study, we report the whole-genome sequence analysis of marine gastropod mollusk <i>Planaxis</i> sp.-associated <i>Streptomyces griseus</i> SCSIO PteL053, which harbors 28 putative biosynthetic gene clusters (BGCs). Among them, two BGCs encoded by a hybrid non-ribosomal peptide (NRPS)/polyketide (PKS) synthetase and non-ribosomal peptide synthetase (NRPS) are responsible for the synthesis of the known therapeutic metabolites 2,2′-bipyridine and actinomycin analogs, respectively. Detailed bioinformatics analysis revealed the putative BGCs and the functions of the involved genes in the biosynthesis of the known compounds SF2738D (<b>1</b>), SF2738F (<b>2</b>), actinomycin D (<b>3</b>), and Actinomycin X<sub>oβ</sub> (<b>4</b>). In the present study, complete-genome sequencing allowed us to rediscover known, clinically useful secondary metabolites in the newly isolated <i>Streptomyces griseus</i> SCSIO PteL053. |
| first_indexed | 2024-03-11T08:36:08Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-11T08:36:08Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-d90fe5069e224818b2b03a9fd92061fe2023-11-16T21:28:33ZengMDPI AGJournal of Marine Science and Engineering2077-13122023-02-0111239610.3390/jmse11020396Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster AnalysisGanesan Govindarajan0Ziwei Yao1Zhenbin Zhou2Xiaohong Zheng3Junying Ma4Pachaiyappan Saravana Kumar5Jianhua Ju6Changli Sun7CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaCAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Centre for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, ChinaMarine symbiotic actinomycetes play a key role in drug development and their ecological niches can influence a variety of natural product biosynthesis, providing potential defensive benefits. In this study, we report the whole-genome sequence analysis of marine gastropod mollusk <i>Planaxis</i> sp.-associated <i>Streptomyces griseus</i> SCSIO PteL053, which harbors 28 putative biosynthetic gene clusters (BGCs). Among them, two BGCs encoded by a hybrid non-ribosomal peptide (NRPS)/polyketide (PKS) synthetase and non-ribosomal peptide synthetase (NRPS) are responsible for the synthesis of the known therapeutic metabolites 2,2′-bipyridine and actinomycin analogs, respectively. Detailed bioinformatics analysis revealed the putative BGCs and the functions of the involved genes in the biosynthesis of the known compounds SF2738D (<b>1</b>), SF2738F (<b>2</b>), actinomycin D (<b>3</b>), and Actinomycin X<sub>oβ</sub> (<b>4</b>). In the present study, complete-genome sequencing allowed us to rediscover known, clinically useful secondary metabolites in the newly isolated <i>Streptomyces griseus</i> SCSIO PteL053.https://www.mdpi.com/2077-1312/11/2/396genome sequencingPKS/NRPS2,2′-bipyridineactinomycin |
| spellingShingle | Ganesan Govindarajan Ziwei Yao Zhenbin Zhou Xiaohong Zheng Junying Ma Pachaiyappan Saravana Kumar Jianhua Ju Changli Sun Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster Analysis Journal of Marine Science and Engineering genome sequencing PKS/NRPS 2,2′-bipyridine actinomycin |
| title | Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster Analysis |
| title_full | Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster Analysis |
| title_fullStr | Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster Analysis |
| title_full_unstemmed | Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster Analysis |
| title_short | Genome Sequencing of <i>Streptomyces griseus</i> SCSIO PteL053, the Producer of 2,2′-Bipyridine and Actinomycin Analogs, and Associated Biosynthetic Gene Cluster Analysis |
| title_sort | genome sequencing of i streptomyces griseus i scsio ptel053 the producer of 2 2 bipyridine and actinomycin analogs and associated biosynthetic gene cluster analysis |
| topic | genome sequencing PKS/NRPS 2,2′-bipyridine actinomycin |
| url | https://www.mdpi.com/2077-1312/11/2/396 |
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