Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication
ABSTRACT: Duck plague virus (DPV) pUL48 is a homologous of herpes simplex virus VP16, and some studies have shown that VP16 is essential for viral replication and proliferation, but there are few studies on DPV pUL48. Therefore, in order to study the function of pUL48 protein, we constructed a UL48-...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122006526 |
| _version_ | 1811175509557510144 |
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| author | Tong Zhou Mingshu Wang Peilin Ruan Dengjian Fan Anchun Cheng Wei Zhang Bin Tian Qiao Yang Ying Wu Shaqiu Zhang Xumin Ou Sai Mao Juan Huang Qun Gao Di Sun Xinxin Zhao Shun Chen Mafeng Liu Dekang Zhu Renyong Jia |
| author_facet | Tong Zhou Mingshu Wang Peilin Ruan Dengjian Fan Anchun Cheng Wei Zhang Bin Tian Qiao Yang Ying Wu Shaqiu Zhang Xumin Ou Sai Mao Juan Huang Qun Gao Di Sun Xinxin Zhao Shun Chen Mafeng Liu Dekang Zhu Renyong Jia |
| author_sort | Tong Zhou |
| collection | DOAJ |
| description | ABSTRACT: Duck plague virus (DPV) pUL48 is a homologous of herpes simplex virus VP16, and some studies have shown that VP16 is essential for viral replication and proliferation, but there are few studies on DPV pUL48. Therefore, in order to study the function of pUL48 protein, we constructed a UL48-deleted mutant (DPV-BAC-∆UL48) that completely reemoved the UL48 gene from the DPV BAC genome and the revertant virus (DPV-BAC-∆UL48R) by using the 2-step red recombination system. Compared with the parental virus (DPV-BAC) and the revertant virus, the titer of UL48-deleted mutant was reduced by more than 38.2%, and the efficiency of producing infectious virions was significantly reduced. In addition, the average size of plaques produced by UL48-deleted mutant was about 30% smaller than that of the parental and revertant viruses, suggesting that pUL48 protein affected the cell-to-cell transmission of DPV. Finally, pharmacological inhibition assay showed that pUL48 is a late protein of DPV. In this study, we found that UL48, as a late gene, plays an important role in viral replication by affecting the formation of DPV infectious virion, virus cell-to-cell transmission, and viral genome transcription, which may provide some help for the study of the function of DPV pUL48 protein and the prevention and control of DPV. |
| first_indexed | 2024-04-10T19:38:09Z |
| format | Article |
| id | doaj.art-d9150d59739a468db0a5c0a0adea3164 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T19:38:09Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-d9150d59739a468db0a5c0a0adea31642023-01-30T04:11:24ZengElsevierPoultry Science0032-57912023-02-011022102358Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replicationTong Zhou0Mingshu Wang1Peilin Ruan2Dengjian Fan3Anchun Cheng4Wei Zhang5Bin Tian6Qiao Yang7Ying Wu8Shaqiu Zhang9Xumin Ou10Sai Mao11Juan Huang12Qun Gao13Di Sun14Xinxin Zhao15Shun Chen16Mafeng Liu17Dekang Zhu18Renyong Jia19Avian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, China; Corresponding author:Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaInstitute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaAvian Center of Disease Research, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Institute of Preventive Veterinary Medicine, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan Povince, 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu City, Sichuan Povince, 611130, ChinaABSTRACT: Duck plague virus (DPV) pUL48 is a homologous of herpes simplex virus VP16, and some studies have shown that VP16 is essential for viral replication and proliferation, but there are few studies on DPV pUL48. Therefore, in order to study the function of pUL48 protein, we constructed a UL48-deleted mutant (DPV-BAC-∆UL48) that completely reemoved the UL48 gene from the DPV BAC genome and the revertant virus (DPV-BAC-∆UL48R) by using the 2-step red recombination system. Compared with the parental virus (DPV-BAC) and the revertant virus, the titer of UL48-deleted mutant was reduced by more than 38.2%, and the efficiency of producing infectious virions was significantly reduced. In addition, the average size of plaques produced by UL48-deleted mutant was about 30% smaller than that of the parental and revertant viruses, suggesting that pUL48 protein affected the cell-to-cell transmission of DPV. Finally, pharmacological inhibition assay showed that pUL48 is a late protein of DPV. In this study, we found that UL48, as a late gene, plays an important role in viral replication by affecting the formation of DPV infectious virion, virus cell-to-cell transmission, and viral genome transcription, which may provide some help for the study of the function of DPV pUL48 protein and the prevention and control of DPV.http://www.sciencedirect.com/science/article/pii/S0032579122006526duck plague viruspUL48 proteinlate geneviral replication |
| spellingShingle | Tong Zhou Mingshu Wang Peilin Ruan Dengjian Fan Anchun Cheng Wei Zhang Bin Tian Qiao Yang Ying Wu Shaqiu Zhang Xumin Ou Sai Mao Juan Huang Qun Gao Di Sun Xinxin Zhao Shun Chen Mafeng Liu Dekang Zhu Renyong Jia Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication Poultry Science duck plague virus pUL48 protein late gene viral replication |
| title | Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication |
| title_full | Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication |
| title_fullStr | Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication |
| title_full_unstemmed | Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication |
| title_short | Research Note: Duck plague virus pUL48 is a late protein that plays an important role in viral replication |
| title_sort | research note duck plague virus pul48 is a late protein that plays an important role in viral replication |
| topic | duck plague virus pUL48 protein late gene viral replication |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122006526 |
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