Live imaging Arabidopsis thaliana embryos under different hydration conditions
Summary: Despite the ecological and agronomical importance of seed germination, how seeds integrate environmental signals to trigger germination remains enigmatic. Recently we reported that a protein called FLOE1 is involved in sensing and responding to water availability during germination. Here, w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721007310 |
| _version_ | 1819099584022970368 |
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| author | Yanniv Dorone Steven Boeynaems Seung Y. Rhee |
| author_facet | Yanniv Dorone Steven Boeynaems Seung Y. Rhee |
| author_sort | Yanniv Dorone |
| collection | DOAJ |
| description | Summary: Despite the ecological and agronomical importance of seed germination, how seeds integrate environmental signals to trigger germination remains enigmatic. Recently we reported that a protein called FLOE1 is involved in sensing and responding to water availability during germination. Here, we present a live-imaging protocol to assess the subcellular localization of a protein of interest during imbibition of desiccated Arabidopsis thaliana seeds with the goal of understanding protein dynamics during the early stages of water uptake.For complete details on the use and execution of this profile, please refer to Dorone et al. (2021). |
| first_indexed | 2024-12-22T00:49:12Z |
| format | Article |
| id | doaj.art-d9183e333dc1432c90d81dcaf27aaae2 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T00:49:12Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-d9183e333dc1432c90d81dcaf27aaae22022-12-21T18:44:29ZengElsevierSTAR Protocols2666-16672021-12-0124101025Live imaging Arabidopsis thaliana embryos under different hydration conditionsYanniv Dorone0Steven Boeynaems1Seung Y. Rhee2Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA; Department of Biology, Stanford University, Stanford, CA 94305, USA; Corresponding authorDepartment of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA; Corresponding authorDepartment of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, USA; Corresponding authorSummary: Despite the ecological and agronomical importance of seed germination, how seeds integrate environmental signals to trigger germination remains enigmatic. Recently we reported that a protein called FLOE1 is involved in sensing and responding to water availability during germination. Here, we present a live-imaging protocol to assess the subcellular localization of a protein of interest during imbibition of desiccated Arabidopsis thaliana seeds with the goal of understanding protein dynamics during the early stages of water uptake.For complete details on the use and execution of this profile, please refer to Dorone et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721007310Cell BiologyDevelopmental biologyMicroscopyModel OrganismsPlant sciencesMolecular Biology |
| spellingShingle | Yanniv Dorone Steven Boeynaems Seung Y. Rhee Live imaging Arabidopsis thaliana embryos under different hydration conditions STAR Protocols Cell Biology Developmental biology Microscopy Model Organisms Plant sciences Molecular Biology |
| title | Live imaging Arabidopsis thaliana embryos under different hydration conditions |
| title_full | Live imaging Arabidopsis thaliana embryos under different hydration conditions |
| title_fullStr | Live imaging Arabidopsis thaliana embryos under different hydration conditions |
| title_full_unstemmed | Live imaging Arabidopsis thaliana embryos under different hydration conditions |
| title_short | Live imaging Arabidopsis thaliana embryos under different hydration conditions |
| title_sort | live imaging arabidopsis thaliana embryos under different hydration conditions |
| topic | Cell Biology Developmental biology Microscopy Model Organisms Plant sciences Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721007310 |
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