Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes
The use of insecticides known as insect growth regulators, which are considered more selective to natural enemies, may be an alternative to integrated pest management of stink bugs of the main crops in Brazil. The objective of this study was to evaluate histological changes in the reproductive syste...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2017-08-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26661 |
| _version_ | 1818543646771773440 |
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| author | Paulo Sérgio Gimenez Cremonez Daniela de Oliveira Pinheiro Ângela Maria Ferreira Falleiros Pedro Manuel Oliveira Janeiro Neves |
| author_facet | Paulo Sérgio Gimenez Cremonez Daniela de Oliveira Pinheiro Ângela Maria Ferreira Falleiros Pedro Manuel Oliveira Janeiro Neves |
| author_sort | Paulo Sérgio Gimenez Cremonez |
| collection | DOAJ |
| description | The use of insecticides known as insect growth regulators, which are considered more selective to natural enemies, may be an alternative to integrated pest management of stink bugs of the main crops in Brazil. The objective of this study was to evaluate histological changes in the reproductive system of Dichelops melacanthus as well as female fecundity and egg fertility after use of growth-regulating insecticides. The insecticides used were buprofezin (a chitin biosynthesis inhibitor) at a sublethal concentration (LC30 of 2.99 g L-1) and pyriproxyfen (a juvenile hormone analog) at a sublethal concentration (LC30 of 8.35 mL L-1). A completely randomized experimental design was used, with 10 repetitions and 10 insects per experimental unit. Two bioassays were performed: in the first bioassay, fecundity (eggs/female) and fertility (nymph eclosion) of the insects that survived the insecticides were evaluated; in the second bioassay, morphological alterations of the ovarioles of adult females and of the testes of adult male insects that survived the insecticides were evaluated. Buprofezin and pyriproxyfen did not affect the adult sex ratio or female fecundity. Pyriproxyfen reduced the percentage of ecloded nymphs (71.6%) compared with that of the control and buprofezin (96.4 and 90.6%, respectively) treatments and had an ovicidal effect, with direct and indirect action on embryogenesis. Morphological changes were observed in both treatments with buprofezin and pyriproxyfen. The alterations observed in female and male reproductive systems may occur by the action of buprofezin and pyriproxyfen on the morphology of both ovarioles and testes. |
| first_indexed | 2024-12-11T22:38:08Z |
| format | Article |
| id | doaj.art-d91ae3e94aae4c9999ca13bbf1b3fb7f |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-11T22:38:08Z |
| publishDate | 2017-08-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-d91ae3e94aae4c9999ca13bbf1b3fb7f2022-12-22T00:47:53ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592017-08-01384Supl12279229210.5433/1679-0359.2017v38n4Supl1p227915767Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testesPaulo Sérgio Gimenez Cremonez0Daniela de Oliveira Pinheiro1Ângela Maria Ferreira Falleiros2Pedro Manuel Oliveira Janeiro Neves3Universidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaUniversidade Estadual de LondrinaThe use of insecticides known as insect growth regulators, which are considered more selective to natural enemies, may be an alternative to integrated pest management of stink bugs of the main crops in Brazil. The objective of this study was to evaluate histological changes in the reproductive system of Dichelops melacanthus as well as female fecundity and egg fertility after use of growth-regulating insecticides. The insecticides used were buprofezin (a chitin biosynthesis inhibitor) at a sublethal concentration (LC30 of 2.99 g L-1) and pyriproxyfen (a juvenile hormone analog) at a sublethal concentration (LC30 of 8.35 mL L-1). A completely randomized experimental design was used, with 10 repetitions and 10 insects per experimental unit. Two bioassays were performed: in the first bioassay, fecundity (eggs/female) and fertility (nymph eclosion) of the insects that survived the insecticides were evaluated; in the second bioassay, morphological alterations of the ovarioles of adult females and of the testes of adult male insects that survived the insecticides were evaluated. Buprofezin and pyriproxyfen did not affect the adult sex ratio or female fecundity. Pyriproxyfen reduced the percentage of ecloded nymphs (71.6%) compared with that of the control and buprofezin (96.4 and 90.6%, respectively) treatments and had an ovicidal effect, with direct and indirect action on embryogenesis. Morphological changes were observed in both treatments with buprofezin and pyriproxyfen. The alterations observed in female and male reproductive systems may occur by the action of buprofezin and pyriproxyfen on the morphology of both ovarioles and testes.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26661Curva dose-respostaDesregulação endócrinaPercevejo-barriga-verdeInseticidas reguladores de crescimentoMorfologia de órgãos reprodutores. |
| spellingShingle | Paulo Sérgio Gimenez Cremonez Daniela de Oliveira Pinheiro Ângela Maria Ferreira Falleiros Pedro Manuel Oliveira Janeiro Neves Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes Semina: Ciências Agrárias Curva dose-resposta Desregulação endócrina Percevejo-barriga-verde Inseticidas reguladores de crescimento Morfologia de órgãos reprodutores. |
| title | Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes |
| title_full | Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes |
| title_fullStr | Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes |
| title_full_unstemmed | Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes |
| title_short | Performance of reproductive system of Dichelops melacanthus (Hemiptera: Pentatomidae) subjected to buprofezin and pyriproxyfen: morphological analysis of ovarioles and testes |
| title_sort | performance of reproductive system of dichelops melacanthus hemiptera pentatomidae subjected to buprofezin and pyriproxyfen morphological analysis of ovarioles and testes |
| topic | Curva dose-resposta Desregulação endócrina Percevejo-barriga-verde Inseticidas reguladores de crescimento Morfologia de órgãos reprodutores. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26661 |
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