Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf Extract
The excess of free radicals in the wound environment contributes to its stagnation during the inflammatory phase, favoring hard-to-heal wounds. Oxidative stress negatively affects cells and the extracellular matrix, hindering the healing process. In this study, we evaluated the antioxidant and wound...
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MDPI AG
2022-02-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/2/349 |
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| author | Antonio Casado-Diaz José Manuel Moreno-Rojas José Verdú-Soriano José Luis Lázaro-Martínez Leocadio Rodríguez-Mañas Isaac Tunez Manuel La Torre Miriam Berenguer Pérez Feliciano Priego-Capote Gema Pereira-Caro |
| author_facet | Antonio Casado-Diaz José Manuel Moreno-Rojas José Verdú-Soriano José Luis Lázaro-Martínez Leocadio Rodríguez-Mañas Isaac Tunez Manuel La Torre Miriam Berenguer Pérez Feliciano Priego-Capote Gema Pereira-Caro |
| author_sort | Antonio Casado-Diaz |
| collection | DOAJ |
| description | The excess of free radicals in the wound environment contributes to its stagnation during the inflammatory phase, favoring hard-to-heal wounds. Oxidative stress negatively affects cells and the extracellular matrix, hindering the healing process. In this study, we evaluated the antioxidant and wound-healing properties of a novel multifunctional amorphous hydrogel-containing <i>Olea europaea</i> leaf extract (OELE). Five assessments were performed: (i) phenolic compounds characterization in OELE; (ii) absolute antioxidant activity determination in OELE and hydrogel (EHO-85); (iii) antioxidant activity measurement of OELE and (iv) its protective effect on cell viability on human dermal fibroblasts (HDFs) and keratinocytes (HaCaT); and (v) EHO-85 wound-healing-capacity analysis on diabetic mice (db/db; BKS.Cg-m+/+Leprdb). The antioxidant activity of OELE was prominent: 2220, 1558, and 1969 µmol TE/g by DPPH, ABTS, and FRAP assays, respectively. Oxidative stress induced with H<sub>2</sub>O<sub>2</sub> in HDFs and HaCaT was normalized, and their viability increased with OELE co-treatment, thus evidencing a protective role. EHO-85 produced an early and sustained wound-healing stimulating effect superior to controls in diabetic mice. This novel amorphous hydrogel presents an important ROS scavenger capacity due to the high phenolic content of OELE, which protects skin cells from oxidative stress and contributes to the physiological process of wound healing. |
| first_indexed | 2024-03-09T21:14:24Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T21:14:24Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-d920f894f908411da588dc569afdeb382023-11-23T21:37:49ZengMDPI AGPharmaceutics1999-49232022-02-0114234910.3390/pharmaceutics14020349Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf ExtractAntonio Casado-Diaz0José Manuel Moreno-Rojas1José Verdú-Soriano2José Luis Lázaro-Martínez3Leocadio Rodríguez-Mañas4Isaac Tunez5Manuel La Torre6Miriam Berenguer Pérez7Feliciano Priego-Capote8Gema Pereira-Caro9Clinical Management Unit of Endocrinology and Nutrition, Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, SpainDepartment of Community Nursing, Preventive Medicine, Public Health and History of Science, Faculty of Health Sciences, University of Alicante, 03690 Alicante, SpainDiabetic Foot Unit, University Podiatry Clinic, Complutense University of Madrid, 28040 Madrid, SpainConsortium for Biomedical Research in Frailty & Healthy Ageing, CIBERFES, Carlos III Institute of Health, 28029 Madrid, SpainMaimónides Institute of Biomedical Research (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, SpainMaimónides Institute of Biomedical Research (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, SpainDepartment of Community Nursing, Preventive Medicine, Public Health and History of Science, Faculty of Health Sciences, University of Alicante, 03690 Alicante, SpainConsortium for Biomedical Research in Frailty & Healthy Ageing, CIBERFES, Carlos III Institute of Health, 28029 Madrid, SpainMaimónides Institute of Biomedical Research (IMIBIC), Reina Sofía University Hospital, University of Córdoba, 14004 Córdoba, SpainThe excess of free radicals in the wound environment contributes to its stagnation during the inflammatory phase, favoring hard-to-heal wounds. Oxidative stress negatively affects cells and the extracellular matrix, hindering the healing process. In this study, we evaluated the antioxidant and wound-healing properties of a novel multifunctional amorphous hydrogel-containing <i>Olea europaea</i> leaf extract (OELE). Five assessments were performed: (i) phenolic compounds characterization in OELE; (ii) absolute antioxidant activity determination in OELE and hydrogel (EHO-85); (iii) antioxidant activity measurement of OELE and (iv) its protective effect on cell viability on human dermal fibroblasts (HDFs) and keratinocytes (HaCaT); and (v) EHO-85 wound-healing-capacity analysis on diabetic mice (db/db; BKS.Cg-m+/+Leprdb). The antioxidant activity of OELE was prominent: 2220, 1558, and 1969 µmol TE/g by DPPH, ABTS, and FRAP assays, respectively. Oxidative stress induced with H<sub>2</sub>O<sub>2</sub> in HDFs and HaCaT was normalized, and their viability increased with OELE co-treatment, thus evidencing a protective role. EHO-85 produced an early and sustained wound-healing stimulating effect superior to controls in diabetic mice. This novel amorphous hydrogel presents an important ROS scavenger capacity due to the high phenolic content of OELE, which protects skin cells from oxidative stress and contributes to the physiological process of wound healing.https://www.mdpi.com/1999-4923/14/2/349hydrogel<i>Olea europaea</i> leaf extractEHO-85antioxidant activityfree radicalsreactive oxygen species |
| spellingShingle | Antonio Casado-Diaz José Manuel Moreno-Rojas José Verdú-Soriano José Luis Lázaro-Martínez Leocadio Rodríguez-Mañas Isaac Tunez Manuel La Torre Miriam Berenguer Pérez Feliciano Priego-Capote Gema Pereira-Caro Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf Extract Pharmaceutics hydrogel <i>Olea europaea</i> leaf extract EHO-85 antioxidant activity free radicals reactive oxygen species |
| title | Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf Extract |
| title_full | Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf Extract |
| title_fullStr | Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf Extract |
| title_full_unstemmed | Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf Extract |
| title_short | Evaluation of Antioxidant and Wound-Healing Properties of EHO-85, a Novel Multifunctional Amorphous Hydrogel Containing <i>Olea europaea</i> Leaf Extract |
| title_sort | evaluation of antioxidant and wound healing properties of eho 85 a novel multifunctional amorphous hydrogel containing i olea europaea i leaf extract |
| topic | hydrogel <i>Olea europaea</i> leaf extract EHO-85 antioxidant activity free radicals reactive oxygen species |
| url | https://www.mdpi.com/1999-4923/14/2/349 |
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