DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain
African swine fever virus (ASFV) causes high morbidity and mortality in swine (<i>Sus scrofa</i>), for which there is no commercially available vaccine. Recent outbreaks of the virus in Trans-Caucasus countries, Eastern Europe, Belgium and China highlight the urgent need to develop effec...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/7/1/12 |
| _version_ | 1811187224250679296 |
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| author | Sun-Young Sunwoo Daniel Pérez-Núñez Igor Morozov Elena G. Sánchez Natasha N. Gaudreault Jessie D. Trujillo Lina Mur Marisa Nogal Daniel Madden Kinga Urbaniak In Joong Kim Wenjun Ma Yolanda Revilla Juergen A. Richt |
| author_facet | Sun-Young Sunwoo Daniel Pérez-Núñez Igor Morozov Elena G. Sánchez Natasha N. Gaudreault Jessie D. Trujillo Lina Mur Marisa Nogal Daniel Madden Kinga Urbaniak In Joong Kim Wenjun Ma Yolanda Revilla Juergen A. Richt |
| author_sort | Sun-Young Sunwoo |
| collection | DOAJ |
| description | African swine fever virus (ASFV) causes high morbidity and mortality in swine (<i>Sus scrofa</i>), for which there is no commercially available vaccine. Recent outbreaks of the virus in Trans-Caucasus countries, Eastern Europe, Belgium and China highlight the urgent need to develop effective vaccines against ASFV. Previously, we evaluated the immunogenicity of a vaccination strategy designed to test various combinations of ASFV antigens encoded by DNA plasmids and recombinant proteins with the aim to activate both humoral and cellular immunity. Based on our previous results, the objective of this study was to test the combined DNA-protein vaccine strategy using a cocktail of the most immunogenic antigens against virulent ASFV challenge. Pigs were vaccinated three times with a cocktail that included ASFV plasmid DNA (CD2v, p72, p32, +/−p17) and recombinant proteins (p15, p35, p54, +/−p17). Three weeks after the third immunization, all pigs were challenged with the virulent ASFV Armenia 2007 strain. The results showed that vaccinated pigs were not protected from ASFV infection or disease. Compared to the non-vaccinated controls, earlier onset of clinical signs, viremia, and death were observed for the vaccinated animals following virulent ASFV challenge. ASFV induced pathology was also enhanced in the vaccinated pigs. Furthermore, while the vaccinated pigs developed antigen-specific antibodies, immunized pig sera at the time of challenge lacked the capacity to neutralize virus, and instead was observed to enhance ASFV infection in vitro. The results of this work points to a putative immune enhancement mechanism involved in ASFV pathogenesis that warrants further investigation. This pilot study provides insight for the selection of appropriate combinations of ASFV antigens for the development of a rationally-designed, safe, and efficacious vaccine for ASF. |
| first_indexed | 2024-04-11T13:58:37Z |
| format | Article |
| id | doaj.art-d92346661167497ca9c55d38d76f5f68 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-11T13:58:37Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-d92346661167497ca9c55d38d76f5f682022-12-22T04:20:11ZengMDPI AGVaccines2076-393X2019-01-01711210.3390/vaccines7010012vaccines7010012DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 StrainSun-Young Sunwoo0Daniel Pérez-Núñez1Igor Morozov2Elena G. Sánchez3Natasha N. Gaudreault4Jessie D. Trujillo5Lina Mur6Marisa Nogal7Daniel Madden8Kinga Urbaniak9In Joong Kim10Wenjun Ma11Yolanda Revilla12Juergen A. Richt13Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USACBMSO-CSIC-UAM, C/Nicolás Cabrera 1, Campus de Cantoblanco, 28049 Madrid, SpainDepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USACBMSO-CSIC-UAM, C/Nicolás Cabrera 1, Campus de Cantoblanco, 28049 Madrid, SpainDepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USADepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USADepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USACBMSO-CSIC-UAM, C/Nicolás Cabrera 1, Campus de Cantoblanco, 28049 Madrid, SpainDepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USADepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USADepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USADepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USACBMSO-CSIC-UAM, C/Nicolás Cabrera 1, Campus de Cantoblanco, 28049 Madrid, SpainDepartment of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K2224 Mosier Hall, 1800 Denison Ave, Manhattan, KS 66506, USAAfrican swine fever virus (ASFV) causes high morbidity and mortality in swine (<i>Sus scrofa</i>), for which there is no commercially available vaccine. Recent outbreaks of the virus in Trans-Caucasus countries, Eastern Europe, Belgium and China highlight the urgent need to develop effective vaccines against ASFV. Previously, we evaluated the immunogenicity of a vaccination strategy designed to test various combinations of ASFV antigens encoded by DNA plasmids and recombinant proteins with the aim to activate both humoral and cellular immunity. Based on our previous results, the objective of this study was to test the combined DNA-protein vaccine strategy using a cocktail of the most immunogenic antigens against virulent ASFV challenge. Pigs were vaccinated three times with a cocktail that included ASFV plasmid DNA (CD2v, p72, p32, +/−p17) and recombinant proteins (p15, p35, p54, +/−p17). Three weeks after the third immunization, all pigs were challenged with the virulent ASFV Armenia 2007 strain. The results showed that vaccinated pigs were not protected from ASFV infection or disease. Compared to the non-vaccinated controls, earlier onset of clinical signs, viremia, and death were observed for the vaccinated animals following virulent ASFV challenge. ASFV induced pathology was also enhanced in the vaccinated pigs. Furthermore, while the vaccinated pigs developed antigen-specific antibodies, immunized pig sera at the time of challenge lacked the capacity to neutralize virus, and instead was observed to enhance ASFV infection in vitro. The results of this work points to a putative immune enhancement mechanism involved in ASFV pathogenesis that warrants further investigation. This pilot study provides insight for the selection of appropriate combinations of ASFV antigens for the development of a rationally-designed, safe, and efficacious vaccine for ASF.https://www.mdpi.com/2076-393X/7/1/12African swine fever virussubunit vaccineplasmid-expressed antigenrecombinant proteinimmune responseimmunopathologyArmenia 2007 strain |
| spellingShingle | Sun-Young Sunwoo Daniel Pérez-Núñez Igor Morozov Elena G. Sánchez Natasha N. Gaudreault Jessie D. Trujillo Lina Mur Marisa Nogal Daniel Madden Kinga Urbaniak In Joong Kim Wenjun Ma Yolanda Revilla Juergen A. Richt DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain Vaccines African swine fever virus subunit vaccine plasmid-expressed antigen recombinant protein immune response immunopathology Armenia 2007 strain |
| title | DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain |
| title_full | DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain |
| title_fullStr | DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain |
| title_full_unstemmed | DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain |
| title_short | DNA-Protein Vaccination Strategy Does Not Protect from Challenge with African Swine Fever Virus Armenia 2007 Strain |
| title_sort | dna protein vaccination strategy does not protect from challenge with african swine fever virus armenia 2007 strain |
| topic | African swine fever virus subunit vaccine plasmid-expressed antigen recombinant protein immune response immunopathology Armenia 2007 strain |
| url | https://www.mdpi.com/2076-393X/7/1/12 |
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