Effects of Growth Media on the Diversity of Culturable Fungi from Lichens
Microscopic and molecular studies suggest that lichen symbioses contain a plethora of associated fungi. These are potential producers of novel bioactive compounds, but strains isolated on standard media usually represent only a minor subset of these fungi. By using various in vitro growth conditions...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-05-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/5/824 |
| _version_ | 1818023404405522432 |
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| author | Lucia Muggia Theodora Kopun Martin Grube |
| author_facet | Lucia Muggia Theodora Kopun Martin Grube |
| author_sort | Lucia Muggia |
| collection | DOAJ |
| description | Microscopic and molecular studies suggest that lichen symbioses contain a plethora of associated fungi. These are potential producers of novel bioactive compounds, but strains isolated on standard media usually represent only a minor subset of these fungi. By using various in vitro growth conditions we are able to modulate and extend the fraction of culturable lichen-associated fungi. We observed that the presence of iron, glucose, magnesium and potassium in growth media is essential for the successful isolation of members from different taxonomic groups. According to sequence data, most isolates besides the lichen mycobionts belong to the classes Dothideomycetes and Eurotiomycetes. With our approach we can further explore the hidden fungal diversity in lichens to assist in the search of novel compounds. |
| first_indexed | 2024-12-10T03:43:47Z |
| format | Article |
| id | doaj.art-d924c6bb61704dc5b43c462b69121f0e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T03:43:47Z |
| publishDate | 2017-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d924c6bb61704dc5b43c462b69121f0e2022-12-22T02:03:30ZengMDPI AGMolecules1420-30492017-05-0122582410.3390/molecules22050824molecules22050824Effects of Growth Media on the Diversity of Culturable Fungi from LichensLucia Muggia0Theodora Kopun1Martin Grube2Department of Life Sciences, University of Trieste, via Giorgieri 10, 34127 Trieste, ItalyInstitute of Plant Science, Karl-Franzens University of Graz, Holteigasse 6, 8010 Graz, AustriaInstitute of Plant Science, Karl-Franzens University of Graz, Holteigasse 6, 8010 Graz, AustriaMicroscopic and molecular studies suggest that lichen symbioses contain a plethora of associated fungi. These are potential producers of novel bioactive compounds, but strains isolated on standard media usually represent only a minor subset of these fungi. By using various in vitro growth conditions we are able to modulate and extend the fraction of culturable lichen-associated fungi. We observed that the presence of iron, glucose, magnesium and potassium in growth media is essential for the successful isolation of members from different taxonomic groups. According to sequence data, most isolates besides the lichen mycobionts belong to the classes Dothideomycetes and Eurotiomycetes. With our approach we can further explore the hidden fungal diversity in lichens to assist in the search of novel compounds.http://www.mdpi.com/1420-3049/22/5/824DothideomycetesEurotiomycetesLeotiomycetesnuclear ribosomal subunits DNAnutrientsSordariomycetes |
| spellingShingle | Lucia Muggia Theodora Kopun Martin Grube Effects of Growth Media on the Diversity of Culturable Fungi from Lichens Molecules Dothideomycetes Eurotiomycetes Leotiomycetes nuclear ribosomal subunits DNA nutrients Sordariomycetes |
| title | Effects of Growth Media on the Diversity of Culturable Fungi from Lichens |
| title_full | Effects of Growth Media on the Diversity of Culturable Fungi from Lichens |
| title_fullStr | Effects of Growth Media on the Diversity of Culturable Fungi from Lichens |
| title_full_unstemmed | Effects of Growth Media on the Diversity of Culturable Fungi from Lichens |
| title_short | Effects of Growth Media on the Diversity of Culturable Fungi from Lichens |
| title_sort | effects of growth media on the diversity of culturable fungi from lichens |
| topic | Dothideomycetes Eurotiomycetes Leotiomycetes nuclear ribosomal subunits DNA nutrients Sordariomycetes |
| url | http://www.mdpi.com/1420-3049/22/5/824 |
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