A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal Therapy
Radiotherapy (RT) is one of the main clinical therapeutic strategies against cancer. Currently, multiple radiosensitizers aimed at enhancing X-ray absorption in cancer tissues have been developed, while limitations still exist for their further applications, such as poor cellular uptake, hypoxia-ind...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/1/423 |
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| author | Ruixue Ran Sinan Guo Xiaoyu Jiang Zhanyin Qian Zhaoyang Guo Yinsong Wang Mingxin Cao Xiaoying Yang |
| author_facet | Ruixue Ran Sinan Guo Xiaoyu Jiang Zhanyin Qian Zhaoyang Guo Yinsong Wang Mingxin Cao Xiaoying Yang |
| author_sort | Ruixue Ran |
| collection | DOAJ |
| description | Radiotherapy (RT) is one of the main clinical therapeutic strategies against cancer. Currently, multiple radiosensitizers aimed at enhancing X-ray absorption in cancer tissues have been developed, while limitations still exist for their further applications, such as poor cellular uptake, hypoxia-induced radioresistance, and unavoidable damage to adjacent normal body tissues. In order to address these problems, a cell-penetrating TAT peptide (YGRKKRRQRRRC)-modified nanohybrid was constructed by doping high-Z element Au in hollow semiconductor Cu<sub>2−x</sub>Se nanoparticles for combined RT and photothermal therapy (PTT) against breast cancer. The obtained Cu<sub>2−x</sub>Se nanoparticles possessed excellent radiosensitizing properties based on their particular band structures, and high photothermal conversion efficiency beneficial for tumor ablation and promoting RT efficacy. Further doping high-Z element Au deposited more high-energy radiation for better radiosensitizing performance. Conjugation of TAT peptides outside the constructed Cu<sub>2−x</sub>Se/Au nanoparticles facilitated their cellular uptake, thus reducing overdosage-induced side effects. This prepared multifunctional nanohybrid showed powerful suppression effects towards breast cancer, both in vitro and in vivo via integrating enhanced cell penetration and uptake, and combined RT/PTT strategies. |
| first_indexed | 2024-03-09T09:44:08Z |
| format | Article |
| id | doaj.art-d926b2a5dc874dc7bcf5d325e5f4e454 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T09:44:08Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d926b2a5dc874dc7bcf5d325e5f4e4542023-12-02T00:43:10ZengMDPI AGMolecules1420-30492023-01-0128142310.3390/molecules28010423A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal TherapyRuixue Ran0Sinan Guo1Xiaoyu Jiang2Zhanyin Qian3Zhaoyang Guo4Yinsong Wang5Mingxin Cao6Xiaoying Yang7Tianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaTianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaTianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaTianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaTianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaTianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaSchool and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, ChinaTianjin Key Laboratory of Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, ChinaRadiotherapy (RT) is one of the main clinical therapeutic strategies against cancer. Currently, multiple radiosensitizers aimed at enhancing X-ray absorption in cancer tissues have been developed, while limitations still exist for their further applications, such as poor cellular uptake, hypoxia-induced radioresistance, and unavoidable damage to adjacent normal body tissues. In order to address these problems, a cell-penetrating TAT peptide (YGRKKRRQRRRC)-modified nanohybrid was constructed by doping high-Z element Au in hollow semiconductor Cu<sub>2−x</sub>Se nanoparticles for combined RT and photothermal therapy (PTT) against breast cancer. The obtained Cu<sub>2−x</sub>Se nanoparticles possessed excellent radiosensitizing properties based on their particular band structures, and high photothermal conversion efficiency beneficial for tumor ablation and promoting RT efficacy. Further doping high-Z element Au deposited more high-energy radiation for better radiosensitizing performance. Conjugation of TAT peptides outside the constructed Cu<sub>2−x</sub>Se/Au nanoparticles facilitated their cellular uptake, thus reducing overdosage-induced side effects. This prepared multifunctional nanohybrid showed powerful suppression effects towards breast cancer, both in vitro and in vivo via integrating enhanced cell penetration and uptake, and combined RT/PTT strategies.https://www.mdpi.com/1420-3049/28/1/423radiosensitizerTAT peptideradiotherapyphotothermal therapycombined therapy |
| spellingShingle | Ruixue Ran Sinan Guo Xiaoyu Jiang Zhanyin Qian Zhaoyang Guo Yinsong Wang Mingxin Cao Xiaoying Yang A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal Therapy Molecules radiosensitizer TAT peptide radiotherapy photothermal therapy combined therapy |
| title | A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal Therapy |
| title_full | A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal Therapy |
| title_fullStr | A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal Therapy |
| title_full_unstemmed | A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal Therapy |
| title_short | A Cell-Penetrating Peptide Modified Cu<sub>2−x</sub>Se/Au Nanohybrid with Enhanced Efficacy for Combined Radio-Photothermal Therapy |
| title_sort | cell penetrating peptide modified cu sub 2 x sub se au nanohybrid with enhanced efficacy for combined radio photothermal therapy |
| topic | radiosensitizer TAT peptide radiotherapy photothermal therapy combined therapy |
| url | https://www.mdpi.com/1420-3049/28/1/423 |
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