In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeon
Gas production from several metabolic pathways is a necessary process that accompanies the growth and central metabolism of some microorganisms. However, accurate and rapid nondestructive detection of gas production is still challenging. To this end, gas chromatography (GC) is primarily used, which...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128064/full |
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| author | Ziyu Yin Ziyu Yin Ziyu Yin Rikuan Zheng Rikuan Zheng Lianfu Li Lianfu Li Shichuan Xi Shichuan Xi Zhendong Luan Zhendong Luan Zhendong Luan Chaomin Sun Chaomin Sun Chaomin Sun Xin Zhang Xin Zhang Xin Zhang |
| author_facet | Ziyu Yin Ziyu Yin Ziyu Yin Rikuan Zheng Rikuan Zheng Lianfu Li Lianfu Li Shichuan Xi Shichuan Xi Zhendong Luan Zhendong Luan Zhendong Luan Chaomin Sun Chaomin Sun Chaomin Sun Xin Zhang Xin Zhang Xin Zhang |
| author_sort | Ziyu Yin |
| collection | DOAJ |
| description | Gas production from several metabolic pathways is a necessary process that accompanies the growth and central metabolism of some microorganisms. However, accurate and rapid nondestructive detection of gas production is still challenging. To this end, gas chromatography (GC) is primarily used, which requires sampling and sample preparation. Furthermore, GC is expensive and difficult to operate. Several researchers working on microbial gases are looking forward to a new method to accurately capture the gas trends within a closed system in real-time. In this study, we developed a precise quantitative analysis for headspace gas in Hungate tubes using Raman spectroscopy. This method requires only a controlled focus on the gas portion inside Hungate tubes, enabling nondestructive, real-time, continuous monitoring without the need for sampling. The peak area ratio was selected to establish a calibration curve with nine different CH4–N2 gaseous mixtures and a linear relationship was observed between the peak area ratio of methane to nitrogen and their molar ratios (A(CH4)/A(N2) = 6.0739 × n(CH4)/n(N2)). The results of in situ quantitative analysis using Raman spectroscopy showed good agreement with those of GC in the continuous monitoring of culture experiments of a deep-sea cold seep methanogenic archaeon. This method significantly improves the detection efficiency and shows great potential for in situ quantitative gas detection in microbiology. It can be a powerful complementary tool to GC. |
| first_indexed | 2024-04-09T19:17:18Z |
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| id | doaj.art-d9273d6416f54c4285e7820b1a9ea8c0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T19:17:18Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d9273d6416f54c4285e7820b1a9ea8c02023-04-06T04:46:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11280641128064In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeonZiyu Yin0Ziyu Yin1Ziyu Yin2Rikuan Zheng3Rikuan Zheng4Lianfu Li5Lianfu Li6Shichuan Xi7Shichuan Xi8Zhendong Luan9Zhendong Luan10Zhendong Luan11Chaomin Sun12Chaomin Sun13Chaomin Sun14Xin Zhang15Xin Zhang16Xin Zhang17CAS Key Laboratory of Marine Geology and Environment and CAS Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Geology and Laboratory for Marine Biology and Biotechnology, Pilot Laboratory for Marine Science and Technology, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Marine Geology and Environment and CAS Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Geology and Laboratory for Marine Biology and Biotechnology, Pilot Laboratory for Marine Science and Technology, Qingdao, ChinaCAS Key Laboratory of Marine Geology and Environment and CAS Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Geology and Laboratory for Marine Biology and Biotechnology, Pilot Laboratory for Marine Science and Technology, Qingdao, ChinaCAS Key Laboratory of Marine Geology and Environment and CAS Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Geology and Laboratory for Marine Biology and Biotechnology, Pilot Laboratory for Marine Science and Technology, Qingdao, ChinaCAS Key Laboratory of Marine Geology and Environment and CAS Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Geology and Laboratory for Marine Biology and Biotechnology, Pilot Laboratory for Marine Science and Technology, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Marine Geology and Environment and CAS Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Geology and Laboratory for Marine Biology and Biotechnology, Pilot Laboratory for Marine Science and Technology, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Marine Geology and Environment and CAS Key Laboratory of Experimental Marine Biology and Center of Deep Sea Research, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Geology and Laboratory for Marine Biology and Biotechnology, Pilot Laboratory for Marine Science and Technology, Qingdao, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaGas production from several metabolic pathways is a necessary process that accompanies the growth and central metabolism of some microorganisms. However, accurate and rapid nondestructive detection of gas production is still challenging. To this end, gas chromatography (GC) is primarily used, which requires sampling and sample preparation. Furthermore, GC is expensive and difficult to operate. Several researchers working on microbial gases are looking forward to a new method to accurately capture the gas trends within a closed system in real-time. In this study, we developed a precise quantitative analysis for headspace gas in Hungate tubes using Raman spectroscopy. This method requires only a controlled focus on the gas portion inside Hungate tubes, enabling nondestructive, real-time, continuous monitoring without the need for sampling. The peak area ratio was selected to establish a calibration curve with nine different CH4–N2 gaseous mixtures and a linear relationship was observed between the peak area ratio of methane to nitrogen and their molar ratios (A(CH4)/A(N2) = 6.0739 × n(CH4)/n(N2)). The results of in situ quantitative analysis using Raman spectroscopy showed good agreement with those of GC in the continuous monitoring of culture experiments of a deep-sea cold seep methanogenic archaeon. This method significantly improves the detection efficiency and shows great potential for in situ quantitative gas detection in microbiology. It can be a powerful complementary tool to GC.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128064/fullRaman spectroscopyquantitative analysisin situCH4–N2 gas systemdeep-sea methanogenic archaeaculture experiment |
| spellingShingle | Ziyu Yin Ziyu Yin Ziyu Yin Rikuan Zheng Rikuan Zheng Lianfu Li Lianfu Li Shichuan Xi Shichuan Xi Zhendong Luan Zhendong Luan Zhendong Luan Chaomin Sun Chaomin Sun Chaomin Sun Xin Zhang Xin Zhang Xin Zhang In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeon Frontiers in Microbiology Raman spectroscopy quantitative analysis in situ CH4–N2 gas system deep-sea methanogenic archaea culture experiment |
| title | In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeon |
| title_full | In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeon |
| title_fullStr | In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeon |
| title_full_unstemmed | In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeon |
| title_short | In situ Raman quantitative monitoring of methanogenesis: Culture experiments of a deep-sea cold seep methanogenic archaeon |
| title_sort | in situ raman quantitative monitoring of methanogenesis culture experiments of a deep sea cold seep methanogenic archaeon |
| topic | Raman spectroscopy quantitative analysis in situ CH4–N2 gas system deep-sea methanogenic archaea culture experiment |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1128064/full |
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