Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR
Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymerase chain reaction (PCR) tests, the primer generated from the internal transcribed spacer region one...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2006-06-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200602-0002_rapid-detection-of-ganoderma-disease-of-coconut-and-assessment-of-inhibition-effect-of-various-control-measures.php |
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| author | Muthusamy Karthikeyan Krishnan Radhika Ramanujam Bhaskaran Subramanian Mathiyazhagan Ramasamy Samiyappan Rethinasamy Velazhahan |
| author_facet | Muthusamy Karthikeyan Krishnan Radhika Ramanujam Bhaskaran Subramanian Mathiyazhagan Ramasamy Samiyappan Rethinasamy Velazhahan |
| author_sort | Muthusamy Karthikeyan |
| collection | DOAJ |
| description | Molecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymerase chain reaction (PCR) tests, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 167 bp in size, was used. Apparently healthy palms in two coconut gardens were tested for Ganoderma disease by ELISA test using basidiocarp protein antiserum. Field trials were laid out in these early-diagnosed palms for the management of the disease. Based on the ELISA results, Pseudomonas fluorescens + Trichoderma viride with chitin amended treatments arrested the multiplication of the pathogen and within 6 months showed an optical density (OD) below the level of infected plants. Integrated Disease Management (IDM) and fungicide tridemorph treated palms showed OD values below infection level within 7 months, and T. harzianum and P. fluorescens + T. viride treated palms showed OD values below infection level in 8 months. |
| first_indexed | 2024-04-10T07:56:52Z |
| format | Article |
| id | doaj.art-d928831baf674837832a8cafe4c16a2e |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T07:56:52Z |
| publishDate | 2006-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-d928831baf674837832a8cafe4c16a2e2023-02-23T04:04:36ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412006-06-01422495710.17221/2771-PPSpps-200602-0002Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCRMuthusamy Karthikeyan0Krishnan Radhika1Ramanujam Bhaskaran2Subramanian Mathiyazhagan3Ramasamy Samiyappan4Rethinasamy Velazhahan5Department of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Tamil Nadu, IndiaDepartment of Soil Science and Agricultural Chemistry, Annamalai University, Tamil Nadu, IndiaCoconut Research Station, Tamil Nadu, IndiaDepartment of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Tamil Nadu, IndiaDepartment of Soil Science and Agricultural Chemistry, Annamalai University, Tamil Nadu, IndiaDepartment of Plant Pathology, Center for Plant Protection Studies, Tamil Nadu Agricultural University, Tamil Nadu, IndiaMolecular and immunological methods were applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used. For the polymerase chain reaction (PCR) tests, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 167 bp in size, was used. Apparently healthy palms in two coconut gardens were tested for Ganoderma disease by ELISA test using basidiocarp protein antiserum. Field trials were laid out in these early-diagnosed palms for the management of the disease. Based on the ELISA results, Pseudomonas fluorescens + Trichoderma viride with chitin amended treatments arrested the multiplication of the pathogen and within 6 months showed an optical density (OD) below the level of infected plants. Integrated Disease Management (IDM) and fungicide tridemorph treated palms showed OD values below infection level within 7 months, and T. harzianum and P. fluorescens + T. viride treated palms showed OD values below infection level in 8 months.https://pps.agriculturejournals.cz/artkey/pps-200602-0002_rapid-detection-of-ganoderma-disease-of-coconut-and-assessment-of-inhibition-effect-of-various-control-measures.phpganodermaearly diagnosispcrelisaintegrated disease management |
| spellingShingle | Muthusamy Karthikeyan Krishnan Radhika Ramanujam Bhaskaran Subramanian Mathiyazhagan Ramasamy Samiyappan Rethinasamy Velazhahan Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR Plant Protection Science ganoderma early diagnosis pcr elisa integrated disease management |
| title | Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR |
| title_full | Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR |
| title_fullStr | Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR |
| title_full_unstemmed | Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR |
| title_short | Rapid detection of Ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and PCR |
| title_sort | rapid detection of ganoderma disease of coconut and assessment of inhibition effect of various control measures by immunoassay and pcr |
| topic | ganoderma early diagnosis pcr elisa integrated disease management |
| url | https://pps.agriculturejournals.cz/artkey/pps-200602-0002_rapid-detection-of-ganoderma-disease-of-coconut-and-assessment-of-inhibition-effect-of-various-control-measures.php |
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