Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of Hens
The effects of <i>Hermetia illucens</i> larvae meal (HILM) as a feed supplement on production performance and cecal microflora were studied in 900 Hy-line Brown laying hens. Laying hens (60 weeks old) were randomly divided into four groups. Each group had five replicates, and each replic...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-7381/10/5/364 |
| _version_ | 1797598120137195520 |
|---|---|
| author | Yan Yan Jinjin Zhang Xiaochen Chen Zhanbin Wang |
| author_facet | Yan Yan Jinjin Zhang Xiaochen Chen Zhanbin Wang |
| author_sort | Yan Yan |
| collection | DOAJ |
| description | The effects of <i>Hermetia illucens</i> larvae meal (HILM) as a feed supplement on production performance and cecal microflora were studied in 900 Hy-line Brown laying hens. Laying hens (60 weeks old) were randomly divided into four groups. Each group had five replicates, and each replicate had 45 hens. The control group was fed with a corn–soybean-based diet, and the experimental groups were fed with 1% HILM, 2% HILM, or 3% HILM. Results were as follows: (1) With the increase in HILM level, the laying rate increased linearly (<i>p</i> ≤ 0.05), and the feed/egg and cracked-egg rate decreased linearly (<i>p</i> ≤ 0.05). (2) Community composition analysis showed that the dominant bacteria in each group were <i>Bacteroidetes</i> and <i>Firmicutes</i>, followed by <i>Actinobacteria</i> and <i>Proteobacteria</i>, which accounted for more than 97% of 16S rRNA gene sequence of the total cecal bacteria. (3) Alpha diversity analysis at the operational taxonomic unit classification level showed that the HILM-addition groups had higher community richness and community diversity than the control group. (4) Principal co-ordinates analysis showed that the cecum samples in each group were significantly separated (<i>p</i> ≤ 0.05). At the phylum level, the relative abundance of <i>Bacteroidetes</i> in the HILM addition groups was significantly lower than that in the control group (<i>p</i> < 0.001), and the relative abundance of <i>Firmicutes</i> in the HILM addition groups was significantly higher than that in the control group (<i>p</i> < 0.001). In conclusion, dietary HILM supplementation had a significant effect on the production performance and cecal microflora of laying hens at the late laying period under the conditions of this experiment but had no adverse effect on the intestinal dominant flora. |
| first_indexed | 2024-03-11T03:14:50Z |
| format | Article |
| id | doaj.art-d929a57793934c4ea5b5d13efa5736e3 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T03:14:50Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-d929a57793934c4ea5b5d13efa5736e32023-11-18T03:37:52ZengMDPI AGVeterinary Sciences2306-73812023-05-0110536410.3390/vetsci10050364Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of HensYan Yan0Jinjin Zhang1Xiaochen Chen2Zhanbin Wang3Henan Provincial Academician Workstation of Feed Resource Development and Healthy Livestock, Department of Animal Science and Technology, Henan University of Science and Technology, Luoyang 271023, ChinaHenan Provincial Academician Workstation of Feed Resource Development and Healthy Livestock, Department of Animal Science and Technology, Henan University of Science and Technology, Luoyang 271023, ChinaHenan Provincial Academician Workstation of Feed Resource Development and Healthy Livestock, Department of Animal Science and Technology, Henan University of Science and Technology, Luoyang 271023, ChinaHenan Provincial Academician Workstation of Feed Resource Development and Healthy Livestock, Department of Animal Science and Technology, Henan University of Science and Technology, Luoyang 271023, ChinaThe effects of <i>Hermetia illucens</i> larvae meal (HILM) as a feed supplement on production performance and cecal microflora were studied in 900 Hy-line Brown laying hens. Laying hens (60 weeks old) were randomly divided into four groups. Each group had five replicates, and each replicate had 45 hens. The control group was fed with a corn–soybean-based diet, and the experimental groups were fed with 1% HILM, 2% HILM, or 3% HILM. Results were as follows: (1) With the increase in HILM level, the laying rate increased linearly (<i>p</i> ≤ 0.05), and the feed/egg and cracked-egg rate decreased linearly (<i>p</i> ≤ 0.05). (2) Community composition analysis showed that the dominant bacteria in each group were <i>Bacteroidetes</i> and <i>Firmicutes</i>, followed by <i>Actinobacteria</i> and <i>Proteobacteria</i>, which accounted for more than 97% of 16S rRNA gene sequence of the total cecal bacteria. (3) Alpha diversity analysis at the operational taxonomic unit classification level showed that the HILM-addition groups had higher community richness and community diversity than the control group. (4) Principal co-ordinates analysis showed that the cecum samples in each group were significantly separated (<i>p</i> ≤ 0.05). At the phylum level, the relative abundance of <i>Bacteroidetes</i> in the HILM addition groups was significantly lower than that in the control group (<i>p</i> < 0.001), and the relative abundance of <i>Firmicutes</i> in the HILM addition groups was significantly higher than that in the control group (<i>p</i> < 0.001). In conclusion, dietary HILM supplementation had a significant effect on the production performance and cecal microflora of laying hens at the late laying period under the conditions of this experiment but had no adverse effect on the intestinal dominant flora.https://www.mdpi.com/2306-7381/10/5/364<i>Hermetia illucens</i>larvae mealproduction performancelaying hencecum microflo |
| spellingShingle | Yan Yan Jinjin Zhang Xiaochen Chen Zhanbin Wang Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of Hens Veterinary Sciences <i>Hermetia illucens</i> larvae meal production performance laying hen cecum microflo |
| title | Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of Hens |
| title_full | Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of Hens |
| title_fullStr | Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of Hens |
| title_full_unstemmed | Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of Hens |
| title_short | Effects of Black Soldier Fly Larvae (<i>Hermetia illucens</i> Larvae) Meal on the Production Performance and Cecal Microbiota of Hens |
| title_sort | effects of black soldier fly larvae i hermetia illucens i larvae meal on the production performance and cecal microbiota of hens |
| topic | <i>Hermetia illucens</i> larvae meal production performance laying hen cecum microflo |
| url | https://www.mdpi.com/2306-7381/10/5/364 |
| work_keys_str_mv | AT yanyan effectsofblacksoldierflylarvaeihermetiaillucensilarvaemealontheproductionperformanceandcecalmicrobiotaofhens AT jinjinzhang effectsofblacksoldierflylarvaeihermetiaillucensilarvaemealontheproductionperformanceandcecalmicrobiotaofhens AT xiaochenchen effectsofblacksoldierflylarvaeihermetiaillucensilarvaemealontheproductionperformanceandcecalmicrobiotaofhens AT zhanbinwang effectsofblacksoldierflylarvaeihermetiaillucensilarvaemealontheproductionperformanceandcecalmicrobiotaofhens |