The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo Approaches
<i>Tribulus terrestris</i> L. (Tt) has been recently gaining attention for its pharmacological value, including its neuroprotective activities. In this study, we explore the neuroprotective effects of a <i>Tribulus terrestris</i> extract in a zebrafish (<i>Danio rerio&l...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/17/2/200 |
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| author | Salwa Bouabdallah Ion Brinza Razvan Stefan Boiangiu Mona H. Ibrahim Iasmina Honceriu Amna Al-Maktoum Oana Cioanca Monica Hancianu Amr Amin Mossadok Ben-Attia Lucian Hritcu |
| author_facet | Salwa Bouabdallah Ion Brinza Razvan Stefan Boiangiu Mona H. Ibrahim Iasmina Honceriu Amna Al-Maktoum Oana Cioanca Monica Hancianu Amr Amin Mossadok Ben-Attia Lucian Hritcu |
| author_sort | Salwa Bouabdallah |
| collection | DOAJ |
| description | <i>Tribulus terrestris</i> L. (Tt) has been recently gaining attention for its pharmacological value, including its neuroprotective activities. In this study, we explore the neuroprotective effects of a <i>Tribulus terrestris</i> extract in a zebrafish (<i>Danio rerio</i>) model of scopolamine (SCOP)-induced memory impairment and brain oxidative stress. SCOP, an anticholinergic drug, was employed to replicate fundamental aspects of Alzheimer’s disease (AD) in animal models. The fish were treated with ethanolic leaf extract (ELE) from Tt (1, 3, and 6 mg/L) for 15 days. SCOP (100 µM) was administered 30 min before behavioral tests were conducted. Molecular interactions of the major compounds identified via UPLC-PDA/MS in Tt fractions with the active site of acetylcholinesterase (AChE) were explored via molecular docking analyses. Terrestrosin C, protodioscin, rutin, and saponin C exhibited the most stable binding. The spatial memory performance was assessed using the Y-maze test, and memory recognition was examined using a novel object recognition (NOR) test. Tt extract treatment reversed the altered locomotion patterns that were caused by SCOP administration. Biochemical analyses also verified Tt’s role in inhibiting AChE, improving antioxidant enzyme activities, and reducing oxidative stress markers. The present findings pave the way for future application of Tt as a natural alternative to treat cognitive disorders. |
| first_indexed | 2024-03-07T22:18:20Z |
| format | Article |
| id | doaj.art-d92ceb69d99e429ab230d660b6149ee0 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-07T22:18:20Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-d92ceb69d99e429ab230d660b6149ee02024-02-23T15:30:39ZengMDPI AGPharmaceuticals1424-82472024-02-0117220010.3390/ph17020200The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo ApproachesSalwa Bouabdallah0Ion Brinza1Razvan Stefan Boiangiu2Mona H. Ibrahim3Iasmina Honceriu4Amna Al-Maktoum5Oana Cioanca6Monica Hancianu7Amr Amin8Mossadok Ben-Attia9Lucian Hritcu10Laboratoire de Biosurveillance de l’Environnement (LR01/ES14), Faculté des Sciences de Bizerte, Université de Carthage, Zarzouna 7021, TunisiaDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaDepartment of Pharmaceutical Medicinal Chemistry and Drug Design, Faculty of Pharmacy (Girls), Al-Azha University, Cairo 11884, EgyptDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, RomaniaBiology Department, College of Science, United Arab Emirates University, Al Ain 15551, United Arab EmiratesFaculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaFaculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, RomaniaCollege of Medicine, University of Sharjah, Sharjah P.O. Box 27272, United Arab EmiratesLaboratoire de Biosurveillance de l’Environnement (LR01/ES14), Faculté des Sciences de Bizerte, Université de Carthage, Zarzouna 7021, TunisiaDepartment of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi, 700506 Iasi, Romania<i>Tribulus terrestris</i> L. (Tt) has been recently gaining attention for its pharmacological value, including its neuroprotective activities. In this study, we explore the neuroprotective effects of a <i>Tribulus terrestris</i> extract in a zebrafish (<i>Danio rerio</i>) model of scopolamine (SCOP)-induced memory impairment and brain oxidative stress. SCOP, an anticholinergic drug, was employed to replicate fundamental aspects of Alzheimer’s disease (AD) in animal models. The fish were treated with ethanolic leaf extract (ELE) from Tt (1, 3, and 6 mg/L) for 15 days. SCOP (100 µM) was administered 30 min before behavioral tests were conducted. Molecular interactions of the major compounds identified via UPLC-PDA/MS in Tt fractions with the active site of acetylcholinesterase (AChE) were explored via molecular docking analyses. Terrestrosin C, protodioscin, rutin, and saponin C exhibited the most stable binding. The spatial memory performance was assessed using the Y-maze test, and memory recognition was examined using a novel object recognition (NOR) test. Tt extract treatment reversed the altered locomotion patterns that were caused by SCOP administration. Biochemical analyses also verified Tt’s role in inhibiting AChE, improving antioxidant enzyme activities, and reducing oxidative stress markers. The present findings pave the way for future application of Tt as a natural alternative to treat cognitive disorders.https://www.mdpi.com/1424-8247/17/2/200Alzheimer’s diseaseAyurvedaneuroprotectionneurobiologyNORnutraceuticals |
| spellingShingle | Salwa Bouabdallah Ion Brinza Razvan Stefan Boiangiu Mona H. Ibrahim Iasmina Honceriu Amna Al-Maktoum Oana Cioanca Monica Hancianu Amr Amin Mossadok Ben-Attia Lucian Hritcu The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo Approaches Pharmaceuticals Alzheimer’s disease Ayurveda neuroprotection neurobiology NOR nutraceuticals |
| title | The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo Approaches |
| title_full | The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo Approaches |
| title_fullStr | The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo Approaches |
| title_full_unstemmed | The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo Approaches |
| title_short | The Effect of a <i>Tribulus</i>-Based Formulation in Alleviating Cholinergic System Impairment and Scopolamine-Induced Memory Loss in Zebrafish (<i>Danio rerio</i>): Insights from Molecular Docking and In Vitro/In Vivo Approaches |
| title_sort | effect of a i tribulus i based formulation in alleviating cholinergic system impairment and scopolamine induced memory loss in zebrafish i danio rerio i insights from molecular docking and in vitro in vivo approaches |
| topic | Alzheimer’s disease Ayurveda neuroprotection neurobiology NOR nutraceuticals |
| url | https://www.mdpi.com/1424-8247/17/2/200 |
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