AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClelland
The ability of eel (Anguilla bicolor McClelland) to digest feed protein is highly dependent on the alkaline proteases in the intestine which are trypsin-like and chymotrypsin-like. Information on the trypsin-like and chymotrypsin-like activities is needed to understand the digestive capacity of eels...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Indonesian |
| Published: |
Jenderal Soedirman University
2018-03-01
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| Series: | Scripta Biologica |
| Subjects: | |
| Online Access: | https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/660 |
| _version_ | 1798045945820086272 |
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| author | Annisa Fitri Larassagita Hana Hana Untung Susilo |
| author_facet | Annisa Fitri Larassagita Hana Hana Untung Susilo |
| author_sort | Annisa Fitri Larassagita |
| collection | DOAJ |
| description | The ability of eel (Anguilla bicolor McClelland) to digest feed protein is highly dependent on the alkaline proteases in the intestine which are trypsin-like and chymotrypsin-like. Information on the trypsin-like and chymotrypsin-like activities is needed to understand the digestive capacity of eels to ingest feed proteins. However, the information on this subject is insufficient. This research aimed to determine the activity of trypsin-like and chymotrypsin-like of eel on the different body weight and incubation temperature. Three groups of eel stadia were selected to represent different body weight, i.e., elver, yellow eel, and pre-silver stadia, and three different enzyme incubation temperatures of 30, 40, and 50°C was applied. The trypsin-like and chymotrypsin-like activity were measured using a spectrophotometry method. The results showed the activities of trypsin-like and chymotrypsin-like were significantly different between the body size and the intestine segment (P < 0.05). The highest trypsin-like and chymotrypsin-like activities were found in the elver stadia approximately 41.25 ± 0.898 g, in the anterior part of the intestine (P < 0.05). The incubation temperatures were not differed significantly (P > 0.05). This study showed the eel had a higher digestive capacity of protein at elver stadium, mainly occur in the anterior part of the intestine, whereas, the temperature ranges from 30 to 50°C had no significant effect on the enzyme activities. |
| first_indexed | 2024-04-11T23:30:33Z |
| format | Article |
| id | doaj.art-d92f1bf735994a87ac5bc4e53e26eb3b |
| institution | Directory Open Access Journal |
| issn | 2355-3138 |
| language | Indonesian |
| last_indexed | 2024-04-11T23:30:33Z |
| publishDate | 2018-03-01 |
| publisher | Jenderal Soedirman University |
| record_format | Article |
| series | Scripta Biologica |
| spelling | doaj.art-d92f1bf735994a87ac5bc4e53e26eb3b2022-12-22T03:57:11ZindJenderal Soedirman UniversityScripta Biologica2355-31382018-03-015110.20884/1.sb.2018.5.1.660404AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClellandAnnisa Fitri Larassagita0Hana HanaUntung SusiloFakultas Biologi Universitas Jenderal SoedirmanThe ability of eel (Anguilla bicolor McClelland) to digest feed protein is highly dependent on the alkaline proteases in the intestine which are trypsin-like and chymotrypsin-like. Information on the trypsin-like and chymotrypsin-like activities is needed to understand the digestive capacity of eels to ingest feed proteins. However, the information on this subject is insufficient. This research aimed to determine the activity of trypsin-like and chymotrypsin-like of eel on the different body weight and incubation temperature. Three groups of eel stadia were selected to represent different body weight, i.e., elver, yellow eel, and pre-silver stadia, and three different enzyme incubation temperatures of 30, 40, and 50°C was applied. The trypsin-like and chymotrypsin-like activity were measured using a spectrophotometry method. The results showed the activities of trypsin-like and chymotrypsin-like were significantly different between the body size and the intestine segment (P < 0.05). The highest trypsin-like and chymotrypsin-like activities were found in the elver stadia approximately 41.25 ± 0.898 g, in the anterior part of the intestine (P < 0.05). The incubation temperatures were not differed significantly (P > 0.05). This study showed the eel had a higher digestive capacity of protein at elver stadium, mainly occur in the anterior part of the intestine, whereas, the temperature ranges from 30 to 50°C had no significant effect on the enzyme activities.https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/660alkalineproteaseeelintestinetemperature |
| spellingShingle | Annisa Fitri Larassagita Hana Hana Untung Susilo AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClelland Scripta Biologica alkaline protease eel intestine temperature |
| title | AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClelland |
| title_full | AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClelland |
| title_fullStr | AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClelland |
| title_full_unstemmed | AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClelland |
| title_short | AKTIVITAS TRIPSIN-<em>LIKE</em> DAN KIMOTRIPSIN-<em>LIKE</em> PADA IKAN SIDAT TROPIK <em>Anguilla bicolor</em> McClelland |
| title_sort | aktivitas tripsin em like em dan kimotripsin em like em pada ikan sidat tropik em anguilla bicolor em mcclelland |
| topic | alkaline protease eel intestine temperature |
| url | https://journal.bio.unsoed.ac.id/index.php/scribio/article/view/660 |
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