Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital
CONTEXT: ICU shows increasing incidence of infection associated with the use of invasive procedures for the diagnostic purpose as well as the indiscriminate use of antibiotics. Pseudomonas aeruginosa and Acinetobacter species are "very successful" pathogen and the emergence of the Metallo-...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2018-04-01
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| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_108_17 |
| _version_ | 1819047898074054656 |
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| author | Jayanthi Siva Subramaniyan Jeya Meenakshi Sundaram |
| author_facet | Jayanthi Siva Subramaniyan Jeya Meenakshi Sundaram |
| author_sort | Jayanthi Siva Subramaniyan |
| collection | DOAJ |
| description | CONTEXT: ICU shows increasing incidence of infection associated with the use of invasive procedures for the diagnostic purpose as well as the indiscriminate use of antibiotics. Pseudomonas aeruginosa and Acinetobacter species are "very successful" pathogen and the emergence of the Metallo-β-Lactamases (MBL) is becoming a therapeutic challenge.
AIMS: To isolate the Nonfermenting Gram negative bacilli from the ICU samples. To identify the metallo betalactamase producers and to detect the bla gene presence among the Pseudomonas aeruginosa and Acinetobacter baumannii.
SETTINGS AND DESIGN: The Nonfermenting Gram negative bacilli isolates from the ICU samples were taken over for 5 years (2009-2014) in a tertiary care hospital.
METHODS AND MATERIALS: The isolates of Pseudomonas species and Acinetobacter species were confirmed by API analyser and processed according to standard procedures. Detection of the MBL producers were done by E strip method and subjected for bla gene detection by PCR method.
RESULTS: In our study a total of 195 isolates of NFGNB were obtained from various ICU. Of these MBL producers, 26 % were Pseudomonas aeruginosa and 25 % were Acinetobacter baumannii. The subtypes of bla
VIMMBL producing P.aeruginosa were 26%.The predominant gene coding for MBL activity in A.baumannii were found to be bla
OXA gene 11.9%. The gene accession numbers were KF975367, KF975372.
CONCLUSIONS: We have to control the development and dissemination of these superbugs among the ICU's. |
| first_indexed | 2024-12-21T11:07:40Z |
| format | Article |
| id | doaj.art-d930c37044de4fcab22e3fa3946b19c7 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-21T11:07:40Z |
| publishDate | 2018-04-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-d930c37044de4fcab22e3fa3946b19c72022-12-21T19:06:11ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262018-04-01100220821310.4103/JLP.JLP_108_17Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospitalJayanthi Siva Subramaniyan0Jeya Meenakshi Sundaram1Department of Microbiology, Chettinad Hospital and Research Institute, Kanchipuram, Tamil Nadu, IndiaDepartment of Microbiology, Chettinad Hospital and Research Institute, Kanchipuram, Tamil Nadu, IndiaCONTEXT: ICU shows increasing incidence of infection associated with the use of invasive procedures for the diagnostic purpose as well as the indiscriminate use of antibiotics. Pseudomonas aeruginosa and Acinetobacter species are "very successful" pathogen and the emergence of the Metallo-β-Lactamases (MBL) is becoming a therapeutic challenge. AIMS: To isolate the Nonfermenting Gram negative bacilli from the ICU samples. To identify the metallo betalactamase producers and to detect the bla gene presence among the Pseudomonas aeruginosa and Acinetobacter baumannii. SETTINGS AND DESIGN: The Nonfermenting Gram negative bacilli isolates from the ICU samples were taken over for 5 years (2009-2014) in a tertiary care hospital. METHODS AND MATERIALS: The isolates of Pseudomonas species and Acinetobacter species were confirmed by API analyser and processed according to standard procedures. Detection of the MBL producers were done by E strip method and subjected for bla gene detection by PCR method. RESULTS: In our study a total of 195 isolates of NFGNB were obtained from various ICU. Of these MBL producers, 26 % were Pseudomonas aeruginosa and 25 % were Acinetobacter baumannii. The subtypes of bla VIMMBL producing P.aeruginosa were 26%.The predominant gene coding for MBL activity in A.baumannii were found to be bla OXA gene 11.9%. The gene accession numbers were KF975367, KF975372. CONCLUSIONS: We have to control the development and dissemination of these superbugs among the ICU's.http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_108_17 acinetobacter baumanni bla genesicumetallo-β-lactamases pseudomonas aeruginosa |
| spellingShingle | Jayanthi Siva Subramaniyan Jeya Meenakshi Sundaram Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital Journal of Laboratory Physicians acinetobacter baumanni bla genes icu metallo-β-lactamases pseudomonas aeruginosa |
| title | Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital |
| title_full | Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital |
| title_fullStr | Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital |
| title_full_unstemmed | Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital |
| title_short | Occurrence of bla genes encoding carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter baumannii from Intensive Care Unit in a tertiary care hospital |
| title_sort | occurrence of bla genes encoding carbapenem resistant pseudomonas aeruginosa and acinetobacter baumannii from intensive care unit in a tertiary care hospital |
| topic | acinetobacter baumanni bla genes icu metallo-β-lactamases pseudomonas aeruginosa |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_108_17 |
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