The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica
Abstract Background Entamoeba histolytica (E. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality. Phagocytic cells and cytokines appear to be important in amoebiasis, but very little is known about the influence of these cells and cytokines in protozoan infec...
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| Format: | Article |
| Language: | English |
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BMC
2015-08-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-1028-6 |
| _version_ | 1797811851955798016 |
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| author | Lucélia Campelo Albuquerque Moraes Eduardo Luzía França Rafael Souza Pessoa Danny Laura Gomes Fagundes Mara Gil Hernandes Victor Pena Ribeiro Maria Aparecida Gomes Adenilda Cristina Honorio-França |
| author_facet | Lucélia Campelo Albuquerque Moraes Eduardo Luzía França Rafael Souza Pessoa Danny Laura Gomes Fagundes Mara Gil Hernandes Victor Pena Ribeiro Maria Aparecida Gomes Adenilda Cristina Honorio-França |
| author_sort | Lucélia Campelo Albuquerque Moraes |
| collection | DOAJ |
| description | Abstract Background Entamoeba histolytica (E. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality. Phagocytic cells and cytokines appear to be important in amoebiasis, but very little is known about the influence of these cells and cytokines in protozoan infections. The aim of this study was to analyse the supernatant of cultures of mononuclear (MN) cells with E. histolytica to determine: 1) the levels of the cytokines IFN-γ and TGF-β, and 2) the amoebicidal activity of MN cells after incubation with cytokines. Methods Blood samples were collected from 30 volunteer donors. The cytokine concentrations in MN cells culture supernatants, superoxide release, leukophagocytosis, amoebicide activity, intracellular calcium release and apoptosis were analysed. Results The IFN-γ concentrations were 6.22 ± 0.36 and TGF-β concentrations were 17.01 ± 2.21 in cells–trophozoite culture supernatants. MN cells, independently of cytokines, in the presence of amoeba increase the superoxide release. In the absence of cytokines, the ingestion of MN cells by amoebae was higher. In the presence of IFN- γ or TGF- β, a lower ingestion of MN cells was observed by amoebae. MN cells treated with cytokines exhibited higher amoebicide and apoptosis indexes. The incubation of cytokines increased the intracellular calcium release by MN cells. Conclusions These results suggest that cytokines play a beneficial role for the host by activating MN cells against E. histolytica. The increased death of amoebae during the leukophagocytosis suggests that both cytokines (IFN-γ and TGF-β) can modulate the functional activity of MN cells and that these cytokines probably are important in the control of amoebic infections. |
| first_indexed | 2024-03-13T07:29:54Z |
| format | Article |
| id | doaj.art-d9364e22caac4526ae69299fc8b87845 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:54Z |
| publishDate | 2015-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-d9364e22caac4526ae69299fc8b878452023-06-04T11:12:38ZengBMCParasites & Vectors1756-33052015-08-01811810.1186/s13071-015-1028-6The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolyticaLucélia Campelo Albuquerque Moraes0Eduardo Luzía França1Rafael Souza Pessoa2Danny Laura Gomes Fagundes3Mara Gil Hernandes4Victor Pena Ribeiro5Maria Aparecida Gomes6Adenilda Cristina Honorio-França7Department of Parasitology, Institute of Biological Sciences, Federal University of Minas GeraisInstitute of Biological and Health Science, Federal University of Mato GrossoInstitute of Biological and Health Science, Federal University of Mato GrossoInstitute of Biological and Health Science, Federal University of Mato GrossoDepartment of Parasitology, Institute of Biological Sciences, Federal University of Minas GeraisInstitute of Biological and Health Science, Federal University of Mato GrossoDepartment of Parasitology, Institute of Biological Sciences, Federal University of Minas GeraisInstitute of Biological and Health Science, Federal University of Mato GrossoAbstract Background Entamoeba histolytica (E. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality. Phagocytic cells and cytokines appear to be important in amoebiasis, but very little is known about the influence of these cells and cytokines in protozoan infections. The aim of this study was to analyse the supernatant of cultures of mononuclear (MN) cells with E. histolytica to determine: 1) the levels of the cytokines IFN-γ and TGF-β, and 2) the amoebicidal activity of MN cells after incubation with cytokines. Methods Blood samples were collected from 30 volunteer donors. The cytokine concentrations in MN cells culture supernatants, superoxide release, leukophagocytosis, amoebicide activity, intracellular calcium release and apoptosis were analysed. Results The IFN-γ concentrations were 6.22 ± 0.36 and TGF-β concentrations were 17.01 ± 2.21 in cells–trophozoite culture supernatants. MN cells, independently of cytokines, in the presence of amoeba increase the superoxide release. In the absence of cytokines, the ingestion of MN cells by amoebae was higher. In the presence of IFN- γ or TGF- β, a lower ingestion of MN cells was observed by amoebae. MN cells treated with cytokines exhibited higher amoebicide and apoptosis indexes. The incubation of cytokines increased the intracellular calcium release by MN cells. Conclusions These results suggest that cytokines play a beneficial role for the host by activating MN cells against E. histolytica. The increased death of amoebae during the leukophagocytosis suggests that both cytokines (IFN-γ and TGF-β) can modulate the functional activity of MN cells and that these cytokines probably are important in the control of amoebic infections.https://doi.org/10.1186/s13071-015-1028-6LeukophagocytosisAmoebicide activityCytokinesMN cellsEntamoeba histolytica |
| spellingShingle | Lucélia Campelo Albuquerque Moraes Eduardo Luzía França Rafael Souza Pessoa Danny Laura Gomes Fagundes Mara Gil Hernandes Victor Pena Ribeiro Maria Aparecida Gomes Adenilda Cristina Honorio-França The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica Parasites & Vectors Leukophagocytosis Amoebicide activity Cytokines MN cells Entamoeba histolytica |
| title | The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica |
| title_full | The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica |
| title_fullStr | The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica |
| title_full_unstemmed | The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica |
| title_short | The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica |
| title_sort | effect of ifn γ and tgf β in the functional activity of mononuclear cells in the presence of entamoeba histolytica |
| topic | Leukophagocytosis Amoebicide activity Cytokines MN cells Entamoeba histolytica |
| url | https://doi.org/10.1186/s13071-015-1028-6 |
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