Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics
Duck hepatitis A virus genotype 3 (DHAV-3) is the major cause of viral hepatitis in ducks in Asia. Previous studies have shown that ducklings younger than 21 days are more susceptible to DHAV-3. To elucidate the mechanism by which age affects the differential susceptibility of Pekin ducks to DHAV-3,...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037024000060 |
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| author | Suyun Liang Meixi Lu Daxin Yu Guangnan Xing Zhanqing Ji Zhanbao Guo Qi Zhang Wei Huang Ming Xie Shuisheng Hou |
| author_facet | Suyun Liang Meixi Lu Daxin Yu Guangnan Xing Zhanqing Ji Zhanbao Guo Qi Zhang Wei Huang Ming Xie Shuisheng Hou |
| author_sort | Suyun Liang |
| collection | DOAJ |
| description | Duck hepatitis A virus genotype 3 (DHAV-3) is the major cause of viral hepatitis in ducks in Asia. Previous studies have shown that ducklings younger than 21 days are more susceptible to DHAV-3. To elucidate the mechanism by which age affects the differential susceptibility of Pekin ducks to DHAV-3, intestinal (n = 520), liver (n = 40) and blood (n = 260) samples were collected from control and DHAV-3-infected ducks at 7, 10, 14, and 21 days of age. Comparisons of plasma markers, mortality rates, and intestinal histopathological data showed that the resistance of Pekin ducks to DHAV-3 varied with age. 16 S sequencing revealed that the ileal microbial composition was influenced by age, and this correlation was greater than that recorded for caecal microbes. Candidatus Arthromitus, Bacteroides, Corynebacterium, Enterococcus, Romboutsia, and Streptococcus were the differntially abundant microbes in the ileum at the genus level after DHAV-3 infection and were significantly correlated with 7 differentially expressed genes (DEGs) in 7- and 21-day-old ducklings. 3 immunity-related pathways were significantly different between 7- and 21-day-old ducklings, especially for IFIH1-mediated induction of the interferon-alpha/beta pathway, which induces differential production of CD8(+) T cells and was influenced by a combination of differentially abundant microbiota and DEGs. We found that microbes in the ileum changed regularly with age. The intestinal microbiota was associated with the expression of genes in the liver through IFIH1-mediated induction of the interferon-alpha/beta pathway, which may partially explain why younger ducklings were more susceptible to DHAV-3 infection. |
| first_indexed | 2024-03-08T11:53:43Z |
| format | Article |
| id | doaj.art-d936c32de75d4f04998bf8bff8b8ae2a |
| institution | Directory Open Access Journal |
| issn | 2001-0370 |
| language | English |
| last_indexed | 2024-03-08T11:53:43Z |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj.art-d936c32de75d4f04998bf8bff8b8ae2a2024-01-24T05:19:41ZengElsevierComputational and Structural Biotechnology Journal2001-03702024-12-0123771782Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomicsSuyun Liang0Meixi Lu1Daxin Yu2Guangnan Xing3Zhanqing Ji4Zhanbao Guo5Qi Zhang6Wei Huang7Ming Xie8Shuisheng Hou9Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Correspondence to: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian, Beijing 100193, China.Duck hepatitis A virus genotype 3 (DHAV-3) is the major cause of viral hepatitis in ducks in Asia. Previous studies have shown that ducklings younger than 21 days are more susceptible to DHAV-3. To elucidate the mechanism by which age affects the differential susceptibility of Pekin ducks to DHAV-3, intestinal (n = 520), liver (n = 40) and blood (n = 260) samples were collected from control and DHAV-3-infected ducks at 7, 10, 14, and 21 days of age. Comparisons of plasma markers, mortality rates, and intestinal histopathological data showed that the resistance of Pekin ducks to DHAV-3 varied with age. 16 S sequencing revealed that the ileal microbial composition was influenced by age, and this correlation was greater than that recorded for caecal microbes. Candidatus Arthromitus, Bacteroides, Corynebacterium, Enterococcus, Romboutsia, and Streptococcus were the differntially abundant microbes in the ileum at the genus level after DHAV-3 infection and were significantly correlated with 7 differentially expressed genes (DEGs) in 7- and 21-day-old ducklings. 3 immunity-related pathways were significantly different between 7- and 21-day-old ducklings, especially for IFIH1-mediated induction of the interferon-alpha/beta pathway, which induces differential production of CD8(+) T cells and was influenced by a combination of differentially abundant microbiota and DEGs. We found that microbes in the ileum changed regularly with age. The intestinal microbiota was associated with the expression of genes in the liver through IFIH1-mediated induction of the interferon-alpha/beta pathway, which may partially explain why younger ducklings were more susceptible to DHAV-3 infection.http://www.sciencedirect.com/science/article/pii/S2001037024000060Pekin duckIntestinal microbiotaAgeDHAV-3Resistance differences |
| spellingShingle | Suyun Liang Meixi Lu Daxin Yu Guangnan Xing Zhanqing Ji Zhanbao Guo Qi Zhang Wei Huang Ming Xie Shuisheng Hou Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics Computational and Structural Biotechnology Journal Pekin duck Intestinal microbiota Age DHAV-3 Resistance differences |
| title | Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics |
| title_full | Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics |
| title_fullStr | Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics |
| title_full_unstemmed | Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics |
| title_short | Effects of age on differential resistance to duck hepatitis A virus genotype 3 in Pekin ducks by 16 S and transcriptomics |
| title_sort | effects of age on differential resistance to duck hepatitis a virus genotype 3 in pekin ducks by 16 s and transcriptomics |
| topic | Pekin duck Intestinal microbiota Age DHAV-3 Resistance differences |
| url | http://www.sciencedirect.com/science/article/pii/S2001037024000060 |
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