Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa.
"Far-West" Africa is known to be a secondary contact zone between the two major malaria vectors Anopheles coluzzii and A. gambiae. We investigated gene-flow and potentially adaptive introgression between these species along a west-to-east transect in Guinea Bissau, the putative core of thi...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4457524?pdf=render |
| _version_ | 1828483060314669056 |
|---|---|
| author | Emiliano Mancini Maria Ida Spinaci Vasco Gordicho Beniamino Caputo Marco Pombi José Luis Vicente João Dinis Amabélia Rodrigues Vincenzo Petrarca David Weetman João Pinto Alessandra Della Torre |
| author_facet | Emiliano Mancini Maria Ida Spinaci Vasco Gordicho Beniamino Caputo Marco Pombi José Luis Vicente João Dinis Amabélia Rodrigues Vincenzo Petrarca David Weetman João Pinto Alessandra Della Torre |
| author_sort | Emiliano Mancini |
| collection | DOAJ |
| description | "Far-West" Africa is known to be a secondary contact zone between the two major malaria vectors Anopheles coluzzii and A. gambiae. We investigated gene-flow and potentially adaptive introgression between these species along a west-to-east transect in Guinea Bissau, the putative core of this hybrid zone. To evaluate the extent and direction of gene flow, we genotyped site 702 in Intron-1 of the para Voltage-Gated Sodium Channel gene, a species-diagnostic nucleotide position throughout most of A. coluzzii and A. gambiae sympatric range. We also analyzed polymorphism in the thioester-binding domain (TED) of the innate immunity-linked thioester-containing protein 1 (TEP1) to investigate whether elevated hybridization might facilitate the exchange of variants linked to adaptive immunity and Plasmodium refractoriness. Our results confirm asymmetric introgression of genetic material from A. coluzzii to A. gambiae and disruption of linkage between the centromeric "genomic islands" of inter-specific divergence. We report that A. gambiae from the Guinean hybrid zone possesses an introgressed TEP1 resistant allelic class, found exclusively in A. coluzzii elsewhere and apparently swept to fixation in West Africa (i.e. Mali and Burkina Faso). However, no detectable fixation of this allele was found in Guinea Bissau, which may suggest that ecological pressures driving segregation between the two species in larval habitats in this region may be different from those experienced in northern and more arid parts of the species' range. Finally, our results also suggest a genetic subdivision between coastal and inland A. gambiae Guinean populations and provide clues on the importance of ecological factors in intra-specific differentiation processes. |
| first_indexed | 2024-12-11T08:29:17Z |
| format | Article |
| id | doaj.art-d93e5c41274d4de19e8a021338a2ae42 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T08:29:17Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d93e5c41274d4de19e8a021338a2ae422022-12-22T01:14:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012780410.1371/journal.pone.0127804Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa.Emiliano ManciniMaria Ida SpinaciVasco GordichoBeniamino CaputoMarco PombiJosé Luis VicenteJoão DinisAmabélia RodriguesVincenzo PetrarcaDavid WeetmanJoão PintoAlessandra Della Torre"Far-West" Africa is known to be a secondary contact zone between the two major malaria vectors Anopheles coluzzii and A. gambiae. We investigated gene-flow and potentially adaptive introgression between these species along a west-to-east transect in Guinea Bissau, the putative core of this hybrid zone. To evaluate the extent and direction of gene flow, we genotyped site 702 in Intron-1 of the para Voltage-Gated Sodium Channel gene, a species-diagnostic nucleotide position throughout most of A. coluzzii and A. gambiae sympatric range. We also analyzed polymorphism in the thioester-binding domain (TED) of the innate immunity-linked thioester-containing protein 1 (TEP1) to investigate whether elevated hybridization might facilitate the exchange of variants linked to adaptive immunity and Plasmodium refractoriness. Our results confirm asymmetric introgression of genetic material from A. coluzzii to A. gambiae and disruption of linkage between the centromeric "genomic islands" of inter-specific divergence. We report that A. gambiae from the Guinean hybrid zone possesses an introgressed TEP1 resistant allelic class, found exclusively in A. coluzzii elsewhere and apparently swept to fixation in West Africa (i.e. Mali and Burkina Faso). However, no detectable fixation of this allele was found in Guinea Bissau, which may suggest that ecological pressures driving segregation between the two species in larval habitats in this region may be different from those experienced in northern and more arid parts of the species' range. Finally, our results also suggest a genetic subdivision between coastal and inland A. gambiae Guinean populations and provide clues on the importance of ecological factors in intra-specific differentiation processes.http://europepmc.org/articles/PMC4457524?pdf=render |
| spellingShingle | Emiliano Mancini Maria Ida Spinaci Vasco Gordicho Beniamino Caputo Marco Pombi José Luis Vicente João Dinis Amabélia Rodrigues Vincenzo Petrarca David Weetman João Pinto Alessandra Della Torre Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa. PLoS ONE |
| title | Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa. |
| title_full | Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa. |
| title_fullStr | Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa. |
| title_full_unstemmed | Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa. |
| title_short | Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa. |
| title_sort | adaptive potential of hybridization among malaria vectors introgression at the immune locus tep1 between anopheles coluzzii and a gambiae in far west africa |
| url | http://europepmc.org/articles/PMC4457524?pdf=render |
| work_keys_str_mv | AT emilianomancini adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT mariaidaspinaci adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT vascogordicho adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT beniaminocaputo adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT marcopombi adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT joseluisvicente adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT joaodinis adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT amabeliarodrigues adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT vincenzopetrarca adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT davidweetman adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT joaopinto adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica AT alessandradellatorre adaptivepotentialofhybridizationamongmalariavectorsintrogressionattheimmunelocustep1betweenanophelescoluzziiandagambiaeinfarwestafrica |