ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> Seedlings
Extracellular ATP (eATP) plays multiple roles in plant growth and development, and stress responses. It has been revealed that eATP suppresses growth and alters the growth orientation of the root and hypocotyl of <i>Arabidopsis thaliana</i> by affecting auxin transport and localization i...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/2/330 |
| _version_ | 1797437672651751424 |
|---|---|
| author | Jiawei Xu Lijuan Han Shuyan Xia Ruojia Zhu Erfang Kang Zhonglin Shang |
| author_facet | Jiawei Xu Lijuan Han Shuyan Xia Ruojia Zhu Erfang Kang Zhonglin Shang |
| author_sort | Jiawei Xu |
| collection | DOAJ |
| description | Extracellular ATP (eATP) plays multiple roles in plant growth and development, and stress responses. It has been revealed that eATP suppresses growth and alters the growth orientation of the root and hypocotyl of <i>Arabidopsis thaliana</i> by affecting auxin transport and localization in these organs. However, the mechanism of the eATP-stimulated auxin distribution remains elusive. Annexins are involved in multiple aspects of plant cellular metabolism, while their role in response to apoplastic signals remains unclear. Here, by using the loss-of-function mutations, we investigated the role of AtANN3 in the eATP-regulated root and hypocotyl growth. Firstly, the inhibitory effects of eATP on root and hypocotyl elongation were weakened or impaired in the <i>AtANN3</i> null mutants (<i>atann3–1</i> and <i>atann3–2</i>). Meanwhile, the distribution of DR5-GUS and DR5-GFP indicated that the eATP-induced asymmetric distribution of auxin in the root tips or hypocotyl cells occurred in wild-type control plants, while in <i>atann3–1</i> mutant seedlings, it was not observed. Further, the eATP-induced asymmetric distribution of PIN2-GFP in root-tip cells or that of PIN3-GFP in hypocotyl cells was reduced in <i>atann3–1</i> seedlings. Finally, the eATP-induced asymmetric distribution of cytoplasmic vesicles in root-tip cells was impaired in <i>atann3–1</i> seedlings. Based on these results, we suggest that AtANN3 may be involved in eATP-regulated seedling growth by regulating the distribution of auxin and auxin transporters in vegetative organs. |
| first_indexed | 2024-03-09T11:25:58Z |
| format | Article |
| id | doaj.art-d942619b8aa544e3a8d11b9c091cc0db |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T11:25:58Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d942619b8aa544e3a8d11b9c091cc0db2023-12-01T00:05:00ZengMDPI AGPlants2223-77472023-01-0112233010.3390/plants12020330ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> SeedlingsJiawei Xu0Lijuan Han1Shuyan Xia2Ruojia Zhu3Erfang Kang4Zhonglin Shang5Ministry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, ChinaMinistry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, ChinaMinistry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, ChinaMinistry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, ChinaMinistry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, ChinaMinistry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, ChinaExtracellular ATP (eATP) plays multiple roles in plant growth and development, and stress responses. It has been revealed that eATP suppresses growth and alters the growth orientation of the root and hypocotyl of <i>Arabidopsis thaliana</i> by affecting auxin transport and localization in these organs. However, the mechanism of the eATP-stimulated auxin distribution remains elusive. Annexins are involved in multiple aspects of plant cellular metabolism, while their role in response to apoplastic signals remains unclear. Here, by using the loss-of-function mutations, we investigated the role of AtANN3 in the eATP-regulated root and hypocotyl growth. Firstly, the inhibitory effects of eATP on root and hypocotyl elongation were weakened or impaired in the <i>AtANN3</i> null mutants (<i>atann3–1</i> and <i>atann3–2</i>). Meanwhile, the distribution of DR5-GUS and DR5-GFP indicated that the eATP-induced asymmetric distribution of auxin in the root tips or hypocotyl cells occurred in wild-type control plants, while in <i>atann3–1</i> mutant seedlings, it was not observed. Further, the eATP-induced asymmetric distribution of PIN2-GFP in root-tip cells or that of PIN3-GFP in hypocotyl cells was reduced in <i>atann3–1</i> seedlings. Finally, the eATP-induced asymmetric distribution of cytoplasmic vesicles in root-tip cells was impaired in <i>atann3–1</i> seedlings. Based on these results, we suggest that AtANN3 may be involved in eATP-regulated seedling growth by regulating the distribution of auxin and auxin transporters in vegetative organs.https://www.mdpi.com/2223-7747/12/2/330extracellular ATP (eATP)<i>Arabidopsis thaliana</i>AtANN3auxinseedling growth |
| spellingShingle | Jiawei Xu Lijuan Han Shuyan Xia Ruojia Zhu Erfang Kang Zhonglin Shang ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> Seedlings Plants extracellular ATP (eATP) <i>Arabidopsis thaliana</i> AtANN3 auxin seedling growth |
| title | ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> Seedlings |
| title_full | ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> Seedlings |
| title_fullStr | ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> Seedlings |
| title_full_unstemmed | ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> Seedlings |
| title_short | ATANN3 Is Involved in Extracellular ATP-Regulated Auxin Distribution in <i>Arabidopsis thaliana</i> Seedlings |
| title_sort | atann3 is involved in extracellular atp regulated auxin distribution in i arabidopsis thaliana i seedlings |
| topic | extracellular ATP (eATP) <i>Arabidopsis thaliana</i> AtANN3 auxin seedling growth |
| url | https://www.mdpi.com/2223-7747/12/2/330 |
| work_keys_str_mv | AT jiaweixu atann3isinvolvedinextracellularatpregulatedauxindistributioniniarabidopsisthalianaiseedlings AT lijuanhan atann3isinvolvedinextracellularatpregulatedauxindistributioniniarabidopsisthalianaiseedlings AT shuyanxia atann3isinvolvedinextracellularatpregulatedauxindistributioniniarabidopsisthalianaiseedlings AT ruojiazhu atann3isinvolvedinextracellularatpregulatedauxindistributioniniarabidopsisthalianaiseedlings AT erfangkang atann3isinvolvedinextracellularatpregulatedauxindistributioniniarabidopsisthalianaiseedlings AT zhonglinshang atann3isinvolvedinextracellularatpregulatedauxindistributioniniarabidopsisthalianaiseedlings |