| Summary: | Coronaviruses (CoVs) infect a wide range of hosts, including humans, domestic animals, and wildlife, typically causing mild-to-severe respiratory or enteric disease. The main objective of this study was to identify CoV genera and subgenera detected in Peruvian alpacas. Lung lavage specimens were collected from 32 animals aged 1 to 6 weeks. CoVs were identified by using RT-PCR to amplify a pan-CoV conserved region of the RNA-dependent RNA polymerase-encoding gene. A nested PCR was performed to identify <i>β</i>-CoVs. Then, <i>β</i>-CoV-positive samples were subjected to RT-PCR using specific primers to identify the <i>Embecovirus</i> subgenus. Out of 32 analyzed samples, 30 (93.8%) tested positive for at least one CoV genus. <i>β</i>-, <i>α</i>-, or unclassified CoVs were identified in 24 (80%), 1 (3.3%), and 1 (3.3%) of the positive samples, respectively. A CoV genus could not be identified in two (6.7%) samples. A mixture of different CoV genera was detected in two (6.7%) samples: one was co-infected with <i>β</i>- and <i>α</i>-CoVs, and the other contained a β- and an unclassified CoV. A sequence analysis of the amplicons generated by the PCR identified 17 <i>β</i>-CoV strains belonging to the subgenus <i>Embecovirus</i> and two <i>α</i>-CoV strains belonging to <i>Decacovirus</i>. A phylogenetic analysis of two strains revealed a relationship with an unclassified <i>Megaderma</i> BatCoV strain. A subgenus could not be identified in nine <i>β</i>-CoV samples. Our data show a high prevalence and a high genetic diversity of CoV genera and subgenera that infect alpacas, in which the <i>β</i>-CoV subgenus <i>Embecovirus</i> predominated. Our data also suggest a new role for bats in the dissemination and transmission of uncommon CoVs to alpacas raised in rural Peru.
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