<i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms
Dental caries, a preventable disease, is caused by highly-adherent, acid-producing biofilms composed of bacteria and yeasts. Current caries-preventive approaches are ineffective in controlling biofilm development. Recent studies demonstrate definite advantages in using natural compounds such as tran...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/7/1428 |
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| author | Afreen Jailani Shanthini Kalimuthu Vidhyashree Rajasekar Sumanta Ghosh Pierre-Yves Collart-Dutilleul Naveen Fatima Hyun Koo Adline Princy Solomon Frederic Cuisinier Prasanna Neelakantan |
| author_facet | Afreen Jailani Shanthini Kalimuthu Vidhyashree Rajasekar Sumanta Ghosh Pierre-Yves Collart-Dutilleul Naveen Fatima Hyun Koo Adline Princy Solomon Frederic Cuisinier Prasanna Neelakantan |
| author_sort | Afreen Jailani |
| collection | DOAJ |
| description | Dental caries, a preventable disease, is caused by highly-adherent, acid-producing biofilms composed of bacteria and yeasts. Current caries-preventive approaches are ineffective in controlling biofilm development. Recent studies demonstrate definite advantages in using natural compounds such as trans-cinnamaldehyde in thwarting biofilm assembly, and yet, the remarkable difficulty in delivering such hydrophobic bioactive molecules prevents further development. To address this critical challenge, we have developed an innovative platform composed of components with a proven track record of safety. We fabricated and thoroughly characterised porous silicon (pSi) microparticles to carry and deliver the natural phenyl propanoid trans-cinnamaldehyde (TC). We investigated its effects on preventing the development of cross-kingdom biofilms (<i>Streptococcus mutans</i> and <i>Candida albicans</i>), typical of dental caries found in children. The prepared pSi microparticles were roughly cubic in structure with 70–75% porosity, to which the TC (pSi-TC) was loaded with about 45% efficiency. The pSi-TC particles exhibited a controlled release of the cargo over a 14-day period. Notably, pSi-TC significantly inhibited biofilms, specifically downregulating the glucan synthesis pathways, leading to reduced adhesion to the substrate. Acid production, a vital virulent trait for caries development, was also hindered by pSi-TC. This pioneering study highlights the potential to develop the novel pSi-TC as a dental caries-preventive material. |
| first_indexed | 2024-03-09T13:11:01Z |
| format | Article |
| id | doaj.art-d943ce82a29e467baf88344eacaa54f0 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T13:11:01Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-d943ce82a29e467baf88344eacaa54f02023-11-30T21:41:57ZengMDPI AGPharmaceutics1999-49232022-07-01147142810.3390/pharmaceutics14071428<i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic BiofilmsAfreen Jailani0Shanthini Kalimuthu1Vidhyashree Rajasekar2Sumanta Ghosh3Pierre-Yves Collart-Dutilleul4Naveen Fatima5Hyun Koo6Adline Princy Solomon7Frederic Cuisinier8Prasanna Neelakantan9Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, ChinaDivision of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, ChinaDivision of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, ChinaDivision of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, ChinaLaboratory of Bioengineering and Nanosciences, University of Montpellier, CEDEX 5, 34193 Montpellier, FranceLaboratory of Bioengineering and Nanosciences, University of Montpellier, CEDEX 5, 34193 Montpellier, FranceBiofilm Research Labs, Department of Orthodontics, Divisions of Pediatric Dentistry and Community Oral Health, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USAQuorum Sensing Laboratory, Centre for Research in Infectious Diseases, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613401, IndiaLaboratory of Bioengineering and Nanosciences, University of Montpellier, CEDEX 5, 34193 Montpellier, FranceDivision of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, ChinaDental caries, a preventable disease, is caused by highly-adherent, acid-producing biofilms composed of bacteria and yeasts. Current caries-preventive approaches are ineffective in controlling biofilm development. Recent studies demonstrate definite advantages in using natural compounds such as trans-cinnamaldehyde in thwarting biofilm assembly, and yet, the remarkable difficulty in delivering such hydrophobic bioactive molecules prevents further development. To address this critical challenge, we have developed an innovative platform composed of components with a proven track record of safety. We fabricated and thoroughly characterised porous silicon (pSi) microparticles to carry and deliver the natural phenyl propanoid trans-cinnamaldehyde (TC). We investigated its effects on preventing the development of cross-kingdom biofilms (<i>Streptococcus mutans</i> and <i>Candida albicans</i>), typical of dental caries found in children. The prepared pSi microparticles were roughly cubic in structure with 70–75% porosity, to which the TC (pSi-TC) was loaded with about 45% efficiency. The pSi-TC particles exhibited a controlled release of the cargo over a 14-day period. Notably, pSi-TC significantly inhibited biofilms, specifically downregulating the glucan synthesis pathways, leading to reduced adhesion to the substrate. Acid production, a vital virulent trait for caries development, was also hindered by pSi-TC. This pioneering study highlights the potential to develop the novel pSi-TC as a dental caries-preventive material.https://www.mdpi.com/1999-4923/14/7/1428acidogenicitybiofilmcariestrans-cinnamaldehyde |
| spellingShingle | Afreen Jailani Shanthini Kalimuthu Vidhyashree Rajasekar Sumanta Ghosh Pierre-Yves Collart-Dutilleul Naveen Fatima Hyun Koo Adline Princy Solomon Frederic Cuisinier Prasanna Neelakantan <i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms Pharmaceutics acidogenicity biofilm caries trans-cinnamaldehyde |
| title | <i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms |
| title_full | <i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms |
| title_fullStr | <i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms |
| title_full_unstemmed | <i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms |
| title_short | <i>Trans</i>-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms |
| title_sort | i trans i cinnamaldehyde eluting porous silicon microparticles mitigate cariogenic biofilms |
| topic | acidogenicity biofilm caries trans-cinnamaldehyde |
| url | https://www.mdpi.com/1999-4923/14/7/1428 |
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