Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin Virome
Swab samples were collected from 34 pangolins in Guangxi Province, China. Metavirome sequencing and bioinformatics approaches were undertaken to determine the abundant viral sequences in the viromes. The results showed that the viral sequences belong to 24 virus taxonomic families. To verify the res...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.874003/full |
| _version_ | 1818531793579540480 |
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| author | Duo Zhang Min Zheng Ying Zhang Guanrong Feng Chengcheng Peng Chenghui Li Yiquan Li He Zhang Nan Li Pengpeng Xiao |
| author_facet | Duo Zhang Min Zheng Ying Zhang Guanrong Feng Chengcheng Peng Chenghui Li Yiquan Li He Zhang Nan Li Pengpeng Xiao |
| author_sort | Duo Zhang |
| collection | DOAJ |
| description | Swab samples were collected from 34 pangolins in Guangxi Province, China. Metavirome sequencing and bioinformatics approaches were undertaken to determine the abundant viral sequences in the viromes. The results showed that the viral sequences belong to 24 virus taxonomic families. To verify the results, PCR combined with phylogenetic analysis was conducted. Some viral sequences including Japanese encephalitis virus (JEV), Getah virus (GETV), and chikungunya virus (CHIKV) were detected. On the basis of the metavirome analysis, seven segments belonging to JEV were further identified through PCR amplification. Sequence comparison showed that, among seven sequences, JEV-China/P2020E-1 displayed the highest nucleotide (80.6%), with the JEV isolated in South Korea, 1988, and all of which belonging to genotype III. Seven CHIKV sequences were detected, with the highest homology (80.6%) to the Aedes africanus in Côte d’Ivoire, 1993. Moreover, passage from BHK-21 to Vero cells makes the newly isolated CHIKV-China/P2020-1 more contagious. In addition, the newly verified GETV sequences shared 86.4% identity with the 1955 GETV isolated from Malaysia. Some sudden and recurrent viruses have also been observed from the virome of pangolin in Guangxi Province, China; hence, dissemination tests will be implemented in the future. |
| first_indexed | 2024-12-11T17:37:05Z |
| format | Article |
| id | doaj.art-d944599f3eb44ab380cc5d7a0c0ba477 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-11T17:37:05Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-d944599f3eb44ab380cc5d7a0c0ba4772022-12-22T00:56:39ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.874003874003Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin ViromeDuo Zhang0Min Zheng1Ying Zhang2Guanrong Feng3Chengcheng Peng4Chenghui Li5Yiquan Li6He Zhang7Nan Li8Pengpeng Xiao9Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, ChinaGuangxi Centre for Animal Disease Control and Prevention, Nanning, ChinaCollege of Veterinary Medicine, College of Animal Science, Jilin University, Changchun, ChinaWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, ChinaWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, ChinaCollege of Agriculture, Yanbian University, Yanji, ChinaAcademician Workstation of Jilin Province, Changchun University of Chinese Medicine, Changchun, ChinaChangchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, ChinaWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, ChinaWenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, ChinaSwab samples were collected from 34 pangolins in Guangxi Province, China. Metavirome sequencing and bioinformatics approaches were undertaken to determine the abundant viral sequences in the viromes. The results showed that the viral sequences belong to 24 virus taxonomic families. To verify the results, PCR combined with phylogenetic analysis was conducted. Some viral sequences including Japanese encephalitis virus (JEV), Getah virus (GETV), and chikungunya virus (CHIKV) were detected. On the basis of the metavirome analysis, seven segments belonging to JEV were further identified through PCR amplification. Sequence comparison showed that, among seven sequences, JEV-China/P2020E-1 displayed the highest nucleotide (80.6%), with the JEV isolated in South Korea, 1988, and all of which belonging to genotype III. Seven CHIKV sequences were detected, with the highest homology (80.6%) to the Aedes africanus in Côte d’Ivoire, 1993. Moreover, passage from BHK-21 to Vero cells makes the newly isolated CHIKV-China/P2020-1 more contagious. In addition, the newly verified GETV sequences shared 86.4% identity with the 1955 GETV isolated from Malaysia. Some sudden and recurrent viruses have also been observed from the virome of pangolin in Guangxi Province, China; hence, dissemination tests will be implemented in the future.https://www.frontiersin.org/articles/10.3389/fcimb.2022.874003/fullmetagenomic analysispangolinmosquito-borne virusviral isolation and identificationphylogenetic analysis |
| spellingShingle | Duo Zhang Min Zheng Ying Zhang Guanrong Feng Chengcheng Peng Chenghui Li Yiquan Li He Zhang Nan Li Pengpeng Xiao Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin Virome Frontiers in Cellular and Infection Microbiology metagenomic analysis pangolin mosquito-borne virus viral isolation and identification phylogenetic analysis |
| title | Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin Virome |
| title_full | Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin Virome |
| title_fullStr | Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin Virome |
| title_full_unstemmed | Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin Virome |
| title_short | Multiple Novel Mosquito-Borne Zoonotic Viruses Revealed in Pangolin Virome |
| title_sort | multiple novel mosquito borne zoonotic viruses revealed in pangolin virome |
| topic | metagenomic analysis pangolin mosquito-borne virus viral isolation and identification phylogenetic analysis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.874003/full |
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