GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE
The present study deals with the application of RAPD markers to study ten varieties of date palm in order to determine the genetic relationship and genetic distance among them. also aimed to determine the DNA fingerprinting for some of them. DNA was isolated from fresh leaves of date palm trees usin...
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| Format: | Article |
| Language: | English |
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University of Anbar
2014-08-01
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| Series: | مجلة جامعة الانبار للعلوم الصرفة |
| Subjects: | |
| Online Access: | https://juaps.uoanbar.edu.iq/article_123975_a22d919e2f1d460493b99aa0a6fbc90e.pdf |
| _version_ | 1797373301802139648 |
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| author | KHALID JAMAL HASHIM ABDUL MAJEED A. HUMADI |
| author_facet | KHALID JAMAL HASHIM ABDUL MAJEED A. HUMADI |
| author_sort | KHALID JAMAL HASHIM |
| collection | DOAJ |
| description | The present study deals with the application of RAPD markers to study ten varieties of date palm in order to determine the genetic relationship and genetic distance among them. also aimed to determine the DNA fingerprinting for some of them. DNA was isolated from fresh leaves of date palm trees using CTAB method. The purity of DNA is ( 1.6-1.85 ) RAPD reactions had been conducted by using 12 primers. Three of them had not been detected, i.e. link sites which include OPN-16, OPZ-7 and OPF-10. Other nine primers show 617 bands 200 of them are main bands and 417 are a variety of Polymorphic bands. The primer OPW 13 has recorded the highest number of binding sites that makes 89 bands. The lower number of binding sites noticed 48 bands of the primer OPN 14. The ( Jouzi) cultivar has recorded the highest number of binding sites making 7 bands. The variety (Barban), however, is characterized with having the least number of binding sites by 40 bands.The results of genetic distance of the RAPD interactions had revealed that the minimum value of the genetic distance (0.0966) are between the two cultivar ( Ashrasee 8) and ( Dakwani 9), the highest value of genetic distance is (0.3214) between the two varieties ( Uwainat Ayyub 6) and ( Braban10). According to these values and regarding to genetic relationship , ten varieties had been classified into three main groups, the first group had included the variety( Ashrasi 8), ( Dakwani 9) and (Braban 10). The second group includes the two varieties ( Um Balaleez 2) and ( Asabia Alaroos 5). The third group includes two single varieties that include ( Assabia Alaroos 5) and ( Jouzi 4) and ( Maya 7). The second group includes the varieties ( Tibarzal, ( Khiarah 3) and( Uwaynat Ayyub 6) |
| first_indexed | 2024-03-08T18:48:27Z |
| format | Article |
| id | doaj.art-d9482bcd818d4c0aa7f03ad6d074e737 |
| institution | Directory Open Access Journal |
| issn | 1991-8941 2706-6703 |
| language | English |
| last_indexed | 2024-03-08T18:48:27Z |
| publishDate | 2014-08-01 |
| publisher | University of Anbar |
| record_format | Article |
| series | مجلة جامعة الانبار للعلوم الصرفة |
| spelling | doaj.art-d9482bcd818d4c0aa7f03ad6d074e7372023-12-28T21:57:27ZengUniversity of Anbarمجلة جامعة الانبار للعلوم الصرفة1991-89412706-67032014-08-0182313910.37652/juaps.2014.123975123975GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUEKHALID JAMAL HASHIM0ABDUL MAJEED A. HUMADI1College ducation university of Al-anbarCollege ducation university of Al-anbarThe present study deals with the application of RAPD markers to study ten varieties of date palm in order to determine the genetic relationship and genetic distance among them. also aimed to determine the DNA fingerprinting for some of them. DNA was isolated from fresh leaves of date palm trees using CTAB method. The purity of DNA is ( 1.6-1.85 ) RAPD reactions had been conducted by using 12 primers. Three of them had not been detected, i.e. link sites which include OPN-16, OPZ-7 and OPF-10. Other nine primers show 617 bands 200 of them are main bands and 417 are a variety of Polymorphic bands. The primer OPW 13 has recorded the highest number of binding sites that makes 89 bands. The lower number of binding sites noticed 48 bands of the primer OPN 14. The ( Jouzi) cultivar has recorded the highest number of binding sites making 7 bands. The variety (Barban), however, is characterized with having the least number of binding sites by 40 bands.The results of genetic distance of the RAPD interactions had revealed that the minimum value of the genetic distance (0.0966) are between the two cultivar ( Ashrasee 8) and ( Dakwani 9), the highest value of genetic distance is (0.3214) between the two varieties ( Uwainat Ayyub 6) and ( Braban10). According to these values and regarding to genetic relationship , ten varieties had been classified into three main groups, the first group had included the variety( Ashrasi 8), ( Dakwani 9) and (Braban 10). The second group includes the two varieties ( Um Balaleez 2) and ( Asabia Alaroos 5). The third group includes two single varieties that include ( Assabia Alaroos 5) and ( Jouzi 4) and ( Maya 7). The second group includes the varieties ( Tibarzal, ( Khiarah 3) and( Uwaynat Ayyub 6)https://juaps.uoanbar.edu.iq/article_123975_a22d919e2f1d460493b99aa0a6fbc90e.pdfrapdpcr |
| spellingShingle | KHALID JAMAL HASHIM ABDUL MAJEED A. HUMADI GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE مجلة جامعة الانبار للعلوم الصرفة rapd pcr |
| title | GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE |
| title_full | GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE |
| title_fullStr | GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE |
| title_full_unstemmed | GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE |
| title_short | GENETIC DIVERSITY ANALYSIS FOR NUMBER OF DATE PALM VARIETIES IN AL-ANBAR USING THE RAPD-PCR TECHNIQUE |
| title_sort | genetic diversity analysis for number of date palm varieties in al anbar using the rapd pcr technique |
| topic | rapd pcr |
| url | https://juaps.uoanbar.edu.iq/article_123975_a22d919e2f1d460493b99aa0a6fbc90e.pdf |
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