Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin B
During inflammation, proinflammatory cytokines are released by various immune cells including IL-6 and activin B. Production of proinflammatory cytokines can be suppressed by estradiol and an increase in estradiol can be found in pregnancy. The aim of this study was to determine the response of IL-6...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Amaltea Medical Publishing House
2023-12-01
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| Series: | Romanian Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | https://rjid.com.ro/articles/2023.4/RJID_2023_4_Art-04.pdf |
| _version_ | 1797253804638339072 |
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| author | Gilang Nugraha Aryati Widjiati Harianto Notopuro Win Darmanto Agus Sulistyono Hari Basuki Notobroto Purwo Sri Rejeki |
| author_facet | Gilang Nugraha Aryati Widjiati Harianto Notopuro Win Darmanto Agus Sulistyono Hari Basuki Notobroto Purwo Sri Rejeki |
| author_sort | Gilang Nugraha |
| collection | DOAJ |
| description | During inflammation, proinflammatory cytokines are released by various immune cells including IL-6 and activin B. Production of proinflammatory cytokines can be suppressed by estradiol and an increase in estradiol can be found in pregnancy. The aim of this study was to determine the response of IL-6 and activin b in pregnant mice through LPS induction. There were two groups of mice consisting of 8 mice which were not pregnant and 13 pregnant mice in the 2nd week. Inflammation was induced by intraperitoneal injection of LPS Escherichia coli serotype O111:B4. After 4 hours of treatment, the mice serum was collected from their intracardiac blood to measure estradiol, IL-6 and activin B using ELISA. Pregnancy significantly reduced IL-6 levels (P=0.015) but there was no significant difference in estradiol (P=0.169) and activin B (P=0.583) levels. We also found that there was no association of E2 with IL-6 (P=0.637) and activin B (P=0.306). Pregnancy influences the inflammatory response, especially IL-6. This condition can occur because during pregnancy physiological changes occur in the body which involve various complex mechanisms in regulating the immune system. |
| first_indexed | 2024-03-07T19:02:47Z |
| format | Article |
| id | doaj.art-d94a558873094028bf68206d7e159e25 |
| institution | Directory Open Access Journal |
| issn | 1454-3389 2069-6051 |
| language | English |
| last_indexed | 2024-04-24T21:39:53Z |
| publishDate | 2023-12-01 |
| publisher | Amaltea Medical Publishing House |
| record_format | Article |
| series | Romanian Journal of Infectious Diseases |
| spelling | doaj.art-d94a558873094028bf68206d7e159e252024-03-21T10:08:00ZengAmaltea Medical Publishing HouseRomanian Journal of Infectious Diseases1454-33892069-60512023-12-0126415015310.37897/RJID.2023.4.4Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin BGilang Nugraha0Aryati1Widjiati2Harianto Notopuro3Win Darmanto4Agus Sulistyono5Hari Basuki Notobroto6Purwo Sri Rejeki7Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, IndonesiaDepartment of Clinical Pathology, School of Medicine, Universitas Airlangga, Surabaya, Indonesia; Institute for Tropical Diseases, Universitas Airlangga, Surabaya, IndonesiaDepartment of Veterinary Anatomy, Faculty of Veterinary Medicine, Surabaya, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, IndonesiaMaternal-Fetal Medicine Division, Obstetrics & Gynecology Department, “Dr. Soetomo” Academic General Hospital, Universitas Airlangga, Surabaya, IndonesiaDepartment of Biostatics & Population, Faculty of Public Health, Universitas Airlangga, Surabaya, IndonesiaDepartment of Physiology, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaDuring inflammation, proinflammatory cytokines are released by various immune cells including IL-6 and activin B. Production of proinflammatory cytokines can be suppressed by estradiol and an increase in estradiol can be found in pregnancy. The aim of this study was to determine the response of IL-6 and activin b in pregnant mice through LPS induction. There were two groups of mice consisting of 8 mice which were not pregnant and 13 pregnant mice in the 2nd week. Inflammation was induced by intraperitoneal injection of LPS Escherichia coli serotype O111:B4. After 4 hours of treatment, the mice serum was collected from their intracardiac blood to measure estradiol, IL-6 and activin B using ELISA. Pregnancy significantly reduced IL-6 levels (P=0.015) but there was no significant difference in estradiol (P=0.169) and activin B (P=0.583) levels. We also found that there was no association of E2 with IL-6 (P=0.637) and activin B (P=0.306). Pregnancy influences the inflammatory response, especially IL-6. This condition can occur because during pregnancy physiological changes occur in the body which involve various complex mechanisms in regulating the immune system.https://rjid.com.ro/articles/2023.4/RJID_2023_4_Art-04.pdfactivin bestradiolinterleukin-6lipopolysaccharide |
| spellingShingle | Gilang Nugraha Aryati Widjiati Harianto Notopuro Win Darmanto Agus Sulistyono Hari Basuki Notobroto Purwo Sri Rejeki Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin B Romanian Journal of Infectious Diseases activin b estradiol interleukin-6 lipopolysaccharide |
| title | Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin B |
| title_full | Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin B |
| title_fullStr | Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin B |
| title_full_unstemmed | Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin B |
| title_short | Induction of lipopolysaccharide during pregnancy will suppress IL-6 production but not activin B |
| title_sort | induction of lipopolysaccharide during pregnancy will suppress il 6 production but not activin b |
| topic | activin b estradiol interleukin-6 lipopolysaccharide |
| url | https://rjid.com.ro/articles/2023.4/RJID_2023_4_Art-04.pdf |
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